Shewanella sp. phage 1/41

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C522|A0A088C522_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/41 OX=1458861 GN=S141_56 PE=4 SV=1
MM1 pKa = 7.16QLVQISAVPSQQFSIPLGGNNYY23 pKa = 9.91DD24 pKa = 3.2IKK26 pKa = 10.96LYY28 pKa = 10.95SIDD31 pKa = 3.24GAMAYY36 pKa = 10.25DD37 pKa = 4.95LDD39 pKa = 4.44INSQRR44 pKa = 11.84LDD46 pKa = 3.6NNGVWLISGFKK57 pKa = 9.58MFNDD61 pKa = 3.44VPLLVYY67 pKa = 10.53RR68 pKa = 11.84SQEE71 pKa = 3.84VNGNLLLQISEE82 pKa = 4.91DD83 pKa = 4.07EE84 pKa = 4.24IPDD87 pKa = 3.4YY88 pKa = 11.35TRR90 pKa = 11.84FGLSQFLYY98 pKa = 10.71YY99 pKa = 9.84LTASEE104 pKa = 4.42AEE106 pKa = 4.35AYY108 pKa = 7.79RR109 pKa = 11.84TVTDD113 pKa = 3.98LL114 pKa = 4.49

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C483|A0A088C483_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/41 OX=1458861 GN=S141_5 PE=4 SV=1
MM1 pKa = 8.03PSTKK5 pKa = 10.2VKK7 pKa = 10.68SKK9 pKa = 9.22VAKK12 pKa = 10.65RR13 pKa = 11.84MDD15 pKa = 3.65RR16 pKa = 11.84PHH18 pKa = 7.04KK19 pKa = 9.6QEE21 pKa = 3.6QMQRR25 pKa = 11.84GHH27 pKa = 7.22DD28 pKa = 3.67KK29 pKa = 10.66LSKK32 pKa = 9.14FFGRR36 pKa = 11.84IGLVII41 pKa = 4.05

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13630

25

905

197.5

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.804 ± 0.451

1.137 ± 0.165

6.456 ± 0.233

6.354 ± 0.385

3.933 ± 0.215

7.124 ± 0.264

1.533 ± 0.153

6.831 ± 0.292

7.014 ± 0.428

7.285 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.192

5.231 ± 0.256

3.375 ± 0.172

3.441 ± 0.199

4.189 ± 0.204

7.219 ± 0.271

5.884 ± 0.318

6.779 ± 0.28

1.284 ± 0.111

3.324 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski