Sphingomonas sp. Leaf339

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6KTG0|A0A0Q6KTG0_9SPHN LysR family transcriptional regulator OS=Sphingomonas sp. Leaf339 OX=1736343 GN=ASG67_16965 PE=3 SV=1
MM1 pKa = 7.52ALSAPATAATLTTAFDD17 pKa = 4.23ADD19 pKa = 3.73NGNDD23 pKa = 3.13GVMFDD28 pKa = 3.82VSVLTGGVSLQSIGINAEE46 pKa = 3.8AGTFDD51 pKa = 3.47YY52 pKa = 11.07AVYY55 pKa = 10.35YY56 pKa = 9.95RR57 pKa = 11.84AGGIGTAVNTPDD69 pKa = 3.32AWTLLGNFNALTTAGLGAVTLLDD92 pKa = 4.32IADD95 pKa = 4.89LDD97 pKa = 4.11LAADD101 pKa = 3.83SLYY104 pKa = 11.06GFYY107 pKa = 10.07VTDD110 pKa = 3.48TQGAFSIAYY119 pKa = 6.74TNSAGSVGDD128 pKa = 3.51VLAADD133 pKa = 3.75EE134 pKa = 4.42RR135 pKa = 11.84LAIGVGYY142 pKa = 10.28GVTYY146 pKa = 11.07AFGDD150 pKa = 3.61ASSPRR155 pKa = 11.84AFNGALNYY163 pKa = 9.62VAAVPEE169 pKa = 4.16PASWAMMIAGFALVGITMRR188 pKa = 11.84RR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84TRR193 pKa = 11.84VVFAA197 pKa = 4.92

Molecular weight: 20.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6KV83|A0A0Q6KV83_9SPHN Acyl-CoA dehydrogenase OS=Sphingomonas sp. Leaf339 OX=1736343 GN=ASG67_06080 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3477

0

3477

1136623

29

1640

326.9

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.736 ± 0.065

0.686 ± 0.011

6.189 ± 0.03

4.642 ± 0.035

3.474 ± 0.028

9.097 ± 0.038

1.91 ± 0.018

4.976 ± 0.025

2.562 ± 0.031

9.619 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.019

2.521 ± 0.037

5.404 ± 0.028

3.008 ± 0.026

7.559 ± 0.046

4.971 ± 0.032

5.944 ± 0.028

7.575 ± 0.035

1.479 ± 0.017

2.252 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski