Halocynthiibacter arcticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Halocynthiibacter

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126V1L2|A0A126V1L2_9RHOB Putrescine-binding periplasmic protein OS=Halocynthiibacter arcticus OX=1579316 GN=RC74_13690 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.64LKK4 pKa = 8.13TTLVGALIASTCLAGMAAAQTDD26 pKa = 3.11ITMWYY31 pKa = 9.0HH32 pKa = 5.71GGGNEE37 pKa = 4.7VEE39 pKa = 4.42SALINQIVSDD49 pKa = 4.49FNAANADD56 pKa = 3.53YY57 pKa = 10.63DD58 pKa = 3.89VSLEE62 pKa = 4.02SFPQGSYY69 pKa = 10.57NDD71 pKa = 3.99SVVAAALAGNLPDD84 pKa = 5.57IIDD87 pKa = 3.24VDD89 pKa = 4.61GPVMPNWAWANYY101 pKa = 6.47MQPLSIDD108 pKa = 3.33EE109 pKa = 4.74SIIADD114 pKa = 4.32FLPGTIGRR122 pKa = 11.84WDD124 pKa = 3.58GEE126 pKa = 4.44LYY128 pKa = 10.89SVGLWDD134 pKa = 4.76AAVSLYY140 pKa = 10.84ARR142 pKa = 11.84QSTLDD147 pKa = 3.89EE148 pKa = 4.55LSLRR152 pKa = 11.84APTLASPWTKK162 pKa = 11.07DD163 pKa = 2.94EE164 pKa = 4.55FMGALEE170 pKa = 4.09AAKK173 pKa = 10.56ASGKK177 pKa = 10.1CEE179 pKa = 3.66YY180 pKa = 11.13AFDD183 pKa = 5.01VGMAWTGEE191 pKa = 3.91WYY193 pKa = 9.24PYY195 pKa = 10.83AFSPFLQSFGGDD207 pKa = 2.53IVDD210 pKa = 3.72RR211 pKa = 11.84STYY214 pKa = 8.3QTAEE218 pKa = 3.77GALNGDD224 pKa = 3.75AAIEE228 pKa = 4.07FGEE231 pKa = 4.16WWQSLFTEE239 pKa = 6.29GYY241 pKa = 8.61TPGTSQDD248 pKa = 3.37PADD251 pKa = 5.0RR252 pKa = 11.84DD253 pKa = 3.62TGFIDD258 pKa = 4.73GKK260 pKa = 11.16YY261 pKa = 9.1CFSWNGNWASVAAIDD276 pKa = 5.1GGVDD280 pKa = 4.21DD281 pKa = 5.8IVFLPAPDD289 pKa = 4.33FGNGSVIGAGSWQFGISATSEE310 pKa = 3.88NAEE313 pKa = 3.93GANAFIEE320 pKa = 4.65FALQDD325 pKa = 3.29KK326 pKa = 10.56YY327 pKa = 10.8MAAFSDD333 pKa = 4.99GIGLIPSTTSAADD346 pKa = 3.15MTEE349 pKa = 4.32NYY351 pKa = 10.32AQGGPLNIYY360 pKa = 9.44FALSAEE366 pKa = 4.25QALVRR371 pKa = 11.84PVSPGYY377 pKa = 10.29VVAAKK382 pKa = 10.53VFEE385 pKa = 4.31KK386 pKa = 10.98ALADD390 pKa = 3.53IANGADD396 pKa = 3.38VADD399 pKa = 4.21TLDD402 pKa = 3.73AAVDD406 pKa = 4.3EE407 pKa = 4.81IDD409 pKa = 3.54TDD411 pKa = 3.54IEE413 pKa = 4.33RR414 pKa = 11.84NSGYY418 pKa = 11.02GHH420 pKa = 7.45

Molecular weight: 44.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126UY85|A0A126UY85_9RHOB Uncharacterized protein OS=Halocynthiibacter arcticus OX=1579316 GN=RC74_06675 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.63HH14 pKa = 4.56RR15 pKa = 11.84HH16 pKa = 4.08GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.43AGRR28 pKa = 11.84NILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3882

0

3882

1158560

37

2470

298.4

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.083 ± 0.047

0.918 ± 0.013

5.746 ± 0.027

5.926 ± 0.037

4.048 ± 0.025

8.068 ± 0.035

2.003 ± 0.018

5.855 ± 0.025

3.988 ± 0.033

9.967 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.021

3.182 ± 0.019

4.688 ± 0.027

3.315 ± 0.024

5.839 ± 0.033

5.968 ± 0.03

5.746 ± 0.03

7.26 ± 0.035

1.306 ± 0.015

2.333 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski