Shewanella sp. phage 1/40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C3M7|A0A088C3M7_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/40 OX=1458860 GN=S140_148 PE=4 SV=1
MM1 pKa = 6.98EE2 pKa = 5.24TIDD5 pKa = 3.76YY6 pKa = 9.51FEE8 pKa = 5.6CRR10 pKa = 11.84DD11 pKa = 3.91CGDD14 pKa = 3.64YY15 pKa = 11.28APDD18 pKa = 4.13GGWCDD23 pKa = 3.25RR24 pKa = 11.84CLDD27 pKa = 3.89DD28 pKa = 5.99VDD30 pKa = 3.56IEE32 pKa = 4.37EE33 pKa = 4.28YY34 pKa = 10.5EE35 pKa = 3.97EE36 pKa = 5.13RR37 pKa = 11.84KK38 pKa = 9.99RR39 pKa = 11.84EE40 pKa = 4.0WLEE43 pKa = 3.8QQEE46 pKa = 4.47DD47 pKa = 3.57

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C4X3|A0A088C4X3_9CAUD Terminase large subunit OS=Shewanella sp. phage 1/40 OX=1458860 GN=S140_95 PE=4 SV=1
MM1 pKa = 7.12VRR3 pKa = 11.84SLNGKK8 pKa = 7.47QCWLAQITSVAAKK21 pKa = 10.47GFVYY25 pKa = 10.35WSRR28 pKa = 11.84LKK30 pKa = 10.78GGRR33 pKa = 11.84IQHH36 pKa = 5.98LTNNTSDD43 pKa = 3.35VSKK46 pKa = 11.05LKK48 pKa = 10.01TYY50 pKa = 11.12SKK52 pKa = 11.41

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

41397

21

855

175.4

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.505 ± 0.191

1.539 ± 0.086

6.517 ± 0.143

6.254 ± 0.19

4.034 ± 0.115

6.735 ± 0.165

1.964 ± 0.083

6.471 ± 0.131

7.136 ± 0.267

8.479 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.095

5.781 ± 0.119

3.08 ± 0.124

3.235 ± 0.095

3.853 ± 0.107

6.824 ± 0.148

6.786 ± 0.201

7.23 ± 0.124

1.435 ± 0.072

4.399 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski