Amino acid dipepetide frequency for Microbotryum intermedium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.296AlaAla: 10.296 ± 0.081
1.078AlaCys: 1.078 ± 0.022
4.125AlaAsp: 4.125 ± 0.039
4.788AlaGlu: 4.788 ± 0.044
3.251AlaPhe: 3.251 ± 0.029
5.697AlaGly: 5.697 ± 0.041
2.17AlaHis: 2.17 ± 0.02
4.012AlaIle: 4.012 ± 0.028
4.143AlaLys: 4.143 ± 0.041
8.74AlaLeu: 8.74 ± 0.06
1.963AlaMet: 1.963 ± 0.022
2.944AlaAsn: 2.944 ± 0.028
6.259AlaPro: 6.259 ± 0.05
3.676AlaGln: 3.676 ± 0.035
5.991AlaArg: 5.991 ± 0.041
10.098AlaSer: 10.098 ± 0.075
6.466AlaThr: 6.466 ± 0.039
5.218AlaVal: 5.218 ± 0.041
1.113AlaTrp: 1.113 ± 0.016
2.02AlaTyr: 2.02 ± 0.023
0.0AlaXaa: 0.0 ± 0.0
Cys
0.937CysAla: 0.937 ± 0.019
0.214CysCys: 0.214 ± 0.008
0.628CysAsp: 0.628 ± 0.02
0.55CysGlu: 0.55 ± 0.013
0.503CysPhe: 0.503 ± 0.011
0.781CysGly: 0.781 ± 0.02
0.297CysHis: 0.297 ± 0.018
0.527CysIle: 0.527 ± 0.012
0.526CysLys: 0.526 ± 0.012
1.191CysLeu: 1.191 ± 0.02
0.196CysMet: 0.196 ± 0.007
0.369CysAsn: 0.369 ± 0.015
0.721CysPro: 0.721 ± 0.016
0.425CysGln: 0.425 ± 0.013
0.705CysArg: 0.705 ± 0.013
1.022CysSer: 1.022 ± 0.02
0.744CysThr: 0.744 ± 0.019
0.79CysVal: 0.79 ± 0.019
0.171CysTrp: 0.171 ± 0.006
0.327CysTyr: 0.327 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
4.975AspAla: 4.975 ± 0.042
0.554AspCys: 0.554 ± 0.021
5.13AspAsp: 5.13 ± 0.072
4.868AspGlu: 4.868 ± 0.052
1.863AspPhe: 1.863 ± 0.024
3.893AspGly: 3.893 ± 0.035
1.415AspHis: 1.415 ± 0.02
2.029AspIle: 2.029 ± 0.023
2.16AspLys: 2.16 ± 0.027
5.207AspLeu: 5.207 ± 0.036
0.897AspMet: 0.897 ± 0.012
1.304AspAsn: 1.304 ± 0.018
3.501AspPro: 3.501 ± 0.029
2.007AspGln: 2.007 ± 0.023
3.292AspArg: 3.292 ± 0.032
4.072AspSer: 4.072 ± 0.039
2.46AspThr: 2.46 ± 0.023
3.469AspVal: 3.469 ± 0.028
0.804AspTrp: 0.804 ± 0.014
1.309AspTyr: 1.309 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
5.517GluAla: 5.517 ± 0.042
0.578GluCys: 0.578 ± 0.012
3.937GluAsp: 3.937 ± 0.044
5.262GluGlu: 5.262 ± 0.062
1.697GluPhe: 1.697 ± 0.02
4.074GluGly: 4.074 ± 0.034
1.367GluHis: 1.367 ± 0.018
2.404GluIle: 2.404 ± 0.027
2.819GluLys: 2.819 ± 0.034
5.307GluLeu: 5.307 ± 0.045
1.408GluMet: 1.408 ± 0.019
1.58GluAsn: 1.58 ± 0.023
2.688GluPro: 2.688 ± 0.029
2.486GluGln: 2.486 ± 0.028
4.288GluArg: 4.288 ± 0.04
3.964GluSer: 3.964 ± 0.032
2.996GluThr: 2.996 ± 0.03
3.57GluVal: 3.57 ± 0.032
0.882GluTrp: 0.882 ± 0.015
1.291GluTyr: 1.291 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.217PheAla: 3.217 ± 0.029
0.497PheCys: 0.497 ± 0.011
2.304PheAsp: 2.304 ± 0.023
2.154PheGlu: 2.154 ± 0.022
1.416PhePhe: 1.416 ± 0.018
2.703PheGly: 2.703 ± 0.034
0.856PheHis: 0.856 ± 0.013
1.516PheIle: 1.516 ± 0.019
1.438PheLys: 1.438 ± 0.021
3.12PheLeu: 3.12 ± 0.035
0.583PheMet: 0.583 ± 0.01
1.132PheAsn: 1.132 ± 0.018
1.848PhePro: 1.848 ± 0.023
1.142PheGln: 1.142 ± 0.016
1.752PheArg: 1.752 ± 0.02
2.89PheSer: 2.89 ± 0.026
1.958PheThr: 1.958 ± 0.025
2.567PheVal: 2.567 ± 0.026
0.51PheTrp: 0.51 ± 0.012
0.863PheTyr: 0.863 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
6.066GlyAla: 6.066 ± 0.045
0.811GlyCys: 0.811 ± 0.016
3.402GlyAsp: 3.402 ± 0.033
3.675GlyGlu: 3.675 ± 0.033
2.517GlyPhe: 2.517 ± 0.03
6.297GlyGly: 6.297 ± 0.062
1.712GlyHis: 1.712 ± 0.021
2.791GlyIle: 2.791 ± 0.031
3.416GlyLys: 3.416 ± 0.035
5.97GlyLeu: 5.97 ± 0.043
1.461GlyMet: 1.461 ± 0.021
1.996GlyAsn: 1.996 ± 0.024
3.487GlyPro: 3.487 ± 0.032
2.447GlyGln: 2.447 ± 0.027
4.275GlyArg: 4.275 ± 0.039
6.237GlySer: 6.237 ± 0.054
4.039GlyThr: 4.039 ± 0.037
4.296GlyVal: 4.296 ± 0.036
1.05GlyTrp: 1.05 ± 0.017
1.67GlyTyr: 1.67 ± 0.023
0.0GlyXaa: 0.0 ± 0.0
His
2.197HisAla: 2.197 ± 0.023
0.344HisCys: 0.344 ± 0.012
1.427HisAsp: 1.427 ± 0.026
1.258HisGlu: 1.258 ± 0.018
0.963HisPhe: 0.963 ± 0.016
1.609HisGly: 1.609 ± 0.021
1.136HisHis: 1.136 ± 0.022
0.947HisIle: 0.947 ± 0.015
0.862HisLys: 0.862 ± 0.015
2.693HisLeu: 2.693 ± 0.025
0.364HisMet: 0.364 ± 0.008
0.668HisAsn: 0.668 ± 0.013
1.96HisPro: 1.96 ± 0.023
1.072HisGln: 1.072 ± 0.016
1.742HisArg: 1.742 ± 0.021
2.204HisSer: 2.204 ± 0.028
1.244HisThr: 1.244 ± 0.016
1.564HisVal: 1.564 ± 0.017
0.303HisTrp: 0.303 ± 0.008
0.673HisTyr: 0.673 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.236IleAla: 4.236 ± 0.033
0.54IleCys: 0.54 ± 0.013
2.832IleAsp: 2.832 ± 0.024
2.83IleGlu: 2.83 ± 0.026
1.517IlePhe: 1.517 ± 0.02
2.807IleGly: 2.807 ± 0.029
0.993IleHis: 0.993 ± 0.013
1.907IleIle: 1.907 ± 0.021
2.028IleLys: 2.028 ± 0.024
3.742IleLeu: 3.742 ± 0.031
0.752IleMet: 0.752 ± 0.011
1.465IleAsn: 1.465 ± 0.019
2.721IlePro: 2.721 ± 0.023
1.484IleGln: 1.484 ± 0.02
2.545IleArg: 2.545 ± 0.028
3.308IleSer: 3.308 ± 0.026
2.339IleThr: 2.339 ± 0.025
3.092IleVal: 3.092 ± 0.028
0.599IleTrp: 0.599 ± 0.013
1.014IleTyr: 1.014 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
4.468LysAla: 4.468 ± 0.042
0.469LysCys: 0.469 ± 0.014
2.364LysAsp: 2.364 ± 0.028
2.991LysGlu: 2.991 ± 0.03
1.236LysPhe: 1.236 ± 0.018
3.17LysGly: 3.17 ± 0.032
1.014LysHis: 1.014 ± 0.015
2.002LysIle: 2.002 ± 0.025
3.083LysLys: 3.083 ± 0.047
3.89LysLeu: 3.89 ± 0.034
0.961LysMet: 0.961 ± 0.015
1.371LysAsn: 1.371 ± 0.017
2.352LysPro: 2.352 ± 0.026
1.588LysGln: 1.588 ± 0.021
3.447LysArg: 3.447 ± 0.037
3.315LysSer: 3.315 ± 0.03
2.697LysThr: 2.697 ± 0.024
2.95LysVal: 2.95 ± 0.033
0.587LysTrp: 0.587 ± 0.013
0.978LysTyr: 0.978 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
8.941LeuAla: 8.941 ± 0.049
1.135LeuCys: 1.135 ± 0.018
5.351LeuAsp: 5.351 ± 0.04
5.384LeuGlu: 5.384 ± 0.042
3.281LeuPhe: 3.281 ± 0.033
6.288LeuGly: 6.288 ± 0.04
2.377LeuHis: 2.377 ± 0.025
3.775LeuIle: 3.775 ± 0.035
3.953LeuLys: 3.953 ± 0.033
8.767LeuLeu: 8.767 ± 0.059
1.665LeuMet: 1.665 ± 0.022
2.952LeuAsn: 2.952 ± 0.024
5.98LeuPro: 5.98 ± 0.038
3.472LeuGln: 3.472 ± 0.031
6.082LeuArg: 6.082 ± 0.047
8.367LeuSer: 8.367 ± 0.054
5.177LeuThr: 5.177 ± 0.035
5.996LeuVal: 5.996 ± 0.046
1.072LeuTrp: 1.072 ± 0.017
2.087LeuTyr: 2.087 ± 0.022
0.0LeuXaa: 0.0 ± 0.0
Met
1.821MetAla: 1.821 ± 0.022
0.233MetCys: 0.233 ± 0.007
1.045MetAsp: 1.045 ± 0.016
0.965MetGlu: 0.965 ± 0.016
0.586MetPhe: 0.586 ± 0.012
1.342MetGly: 1.342 ± 0.021
0.444MetHis: 0.444 ± 0.011
0.845MetIle: 0.845 ± 0.013
0.78MetLys: 0.78 ± 0.015
1.737MetLeu: 1.737 ± 0.025
0.525MetMet: 0.525 ± 0.011
0.608MetAsn: 0.608 ± 0.011
1.11MetPro: 1.11 ± 0.017
0.747MetGln: 0.747 ± 0.014
1.286MetArg: 1.286 ± 0.017
1.863MetSer: 1.863 ± 0.023
1.399MetThr: 1.399 ± 0.015
1.179MetVal: 1.179 ± 0.018
0.225MetTrp: 0.225 ± 0.007
0.451MetTyr: 0.451 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
3.09AsnAla: 3.09 ± 0.031
0.355AsnCys: 0.355 ± 0.015
1.791AsnAsp: 1.791 ± 0.02
1.86AsnGlu: 1.86 ± 0.023
0.986AsnPhe: 0.986 ± 0.017
2.632AsnGly: 2.632 ± 0.033
0.759AsnHis: 0.759 ± 0.014
1.249AsnIle: 1.249 ± 0.018
1.418AsnLys: 1.418 ± 0.022
2.838AsnLeu: 2.838 ± 0.026
0.509AsnMet: 0.509 ± 0.011
1.168AsnAsn: 1.168 ± 0.023
2.12AsnPro: 2.12 ± 0.023
1.119AsnGln: 1.119 ± 0.019
1.848AsnArg: 1.848 ± 0.021
2.614AsnSer: 2.614 ± 0.035
1.676AsnThr: 1.676 ± 0.022
2.031AsnVal: 2.031 ± 0.023
0.418AsnTrp: 0.418 ± 0.009
0.755AsnTyr: 0.755 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
5.635ProAla: 5.635 ± 0.045
0.568ProCys: 0.568 ± 0.011
2.815ProAsp: 2.815 ± 0.026
3.019ProGlu: 3.019 ± 0.027
2.185ProPhe: 2.185 ± 0.024
3.508ProGly: 3.508 ± 0.035
1.562ProHis: 1.562 ± 0.021
2.786ProIle: 2.786 ± 0.028
2.606ProLys: 2.606 ± 0.035
5.638ProLeu: 5.638 ± 0.044
1.11ProMet: 1.11 ± 0.017
2.087ProAsn: 2.087 ± 0.025
6.21ProPro: 6.21 ± 0.067
2.271ProGln: 2.271 ± 0.029
3.859ProArg: 3.859 ± 0.036
8.026ProSer: 8.026 ± 0.067
5.192ProThr: 5.192 ± 0.04
3.559ProVal: 3.559 ± 0.031
0.605ProTrp: 0.605 ± 0.011
1.346ProTyr: 1.346 ± 0.018
0.0ProXaa: 0.0 ± 0.0
Gln
3.854GlnAla: 3.854 ± 0.037
0.415GlnCys: 0.415 ± 0.016
1.883GlnAsp: 1.883 ± 0.024
2.07GlnGlu: 2.07 ± 0.025
1.098GlnPhe: 1.098 ± 0.014
2.565GlnGly: 2.565 ± 0.026
1.24GlnHis: 1.24 ± 0.02
1.573GlnIle: 1.573 ± 0.019
1.403GlnLys: 1.403 ± 0.019
3.563GlnLeu: 3.563 ± 0.032
0.726GlnMet: 0.726 ± 0.015
1.066GlnAsn: 1.066 ± 0.017
2.243GlnPro: 2.243 ± 0.032
2.579GlnGln: 2.579 ± 0.078
2.705GlnArg: 2.705 ± 0.028
2.844GlnSer: 2.844 ± 0.03
2.043GlnThr: 2.043 ± 0.023
2.397GlnVal: 2.397 ± 0.022
0.477GlnTrp: 0.477 ± 0.011
0.826GlnTyr: 0.826 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
5.719ArgAla: 5.719 ± 0.049
0.729ArgCys: 0.729 ± 0.014
3.149ArgAsp: 3.149 ± 0.035
3.618ArgGlu: 3.618 ± 0.036
2.261ArgPhe: 2.261 ± 0.021
4.025ArgGly: 4.025 ± 0.041
1.697ArgHis: 1.697 ± 0.021
2.965ArgIle: 2.965 ± 0.03
3.279ArgLys: 3.279 ± 0.028
6.135ArgLeu: 6.135 ± 0.05
1.343ArgMet: 1.343 ± 0.019
1.926ArgAsn: 1.926 ± 0.023
4.007ArgPro: 4.007 ± 0.036
2.448ArgGln: 2.448 ± 0.023
5.683ArgArg: 5.683 ± 0.055
6.075ArgSer: 6.075 ± 0.051
3.753ArgThr: 3.753 ± 0.03
3.662ArgVal: 3.662 ± 0.027
0.969ArgTrp: 0.969 ± 0.016
1.487ArgTyr: 1.487 ± 0.017
0.0ArgXaa: 0.0 ± 0.0
Ser
8.467SerAla: 8.467 ± 0.057
0.954SerCys: 0.954 ± 0.021
4.45SerAsp: 4.45 ± 0.034
3.936SerGlu: 3.936 ± 0.036
3.259SerPhe: 3.259 ± 0.032
5.774SerGly: 5.774 ± 0.046
2.347SerHis: 2.347 ± 0.03
4.213SerIle: 4.213 ± 0.035
3.879SerLys: 3.879 ± 0.033
8.398SerLeu: 8.398 ± 0.054
1.703SerMet: 1.703 ± 0.021
3.317SerAsn: 3.317 ± 0.036
6.784SerPro: 6.784 ± 0.076
3.026SerGln: 3.026 ± 0.031
5.611SerArg: 5.611 ± 0.047
13.614SerSer: 13.614 ± 0.13
8.207SerThr: 8.207 ± 0.071
4.776SerVal: 4.776 ± 0.037
1.001SerTrp: 1.001 ± 0.017
1.886SerTyr: 1.886 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
5.625ThrAla: 5.625 ± 0.036
0.834ThrCys: 0.834 ± 0.023
2.592ThrAsp: 2.592 ± 0.028
2.726ThrGlu: 2.726 ± 0.025
2.242ThrPhe: 2.242 ± 0.025
3.867ThrGly: 3.867 ± 0.032
1.433ThrHis: 1.433 ± 0.016
2.958ThrIle: 2.958 ± 0.028
2.709ThrLys: 2.709 ± 0.028
5.753ThrLeu: 5.753 ± 0.037
1.158ThrMet: 1.158 ± 0.017
2.102ThrAsn: 2.102 ± 0.021
5.051ThrPro: 5.051 ± 0.043
1.971ThrGln: 1.971 ± 0.024
3.78ThrArg: 3.78 ± 0.034
7.552ThrSer: 7.552 ± 0.067
5.721ThrThr: 5.721 ± 0.058
3.407ThrVal: 3.407 ± 0.029
0.761ThrTrp: 0.761 ± 0.013
1.402ThrTyr: 1.402 ± 0.018
0.0ThrXaa: 0.0 ± 0.0
Val
5.855ValAla: 5.855 ± 0.044
0.789ValCys: 0.789 ± 0.019
3.742ValAsp: 3.742 ± 0.031
4.215ValGlu: 4.215 ± 0.038
2.214ValPhe: 2.214 ± 0.025
4.175ValGly: 4.175 ± 0.033
1.463ValHis: 1.463 ± 0.018
2.539ValIle: 2.539 ± 0.029
2.835ValLys: 2.835 ± 0.028
5.882ValLeu: 5.882 ± 0.044
1.229ValMet: 1.229 ± 0.016
1.899ValAsn: 1.899 ± 0.023
3.779ValPro: 3.779 ± 0.032
2.287ValGln: 2.287 ± 0.023
3.695ValArg: 3.695 ± 0.029
4.605ValSer: 4.605 ± 0.038
3.292ValThr: 3.292 ± 0.03
4.605ValVal: 4.605 ± 0.039
0.846ValTrp: 0.846 ± 0.017
1.417ValTyr: 1.417 ± 0.019
0.0ValXaa: 0.0 ± 0.0
Trp
1.101TrpAla: 1.101 ± 0.019
0.179TrpCys: 0.179 ± 0.007
0.753TrpAsp: 0.753 ± 0.016
0.71TrpGlu: 0.71 ± 0.014
0.43TrpPhe: 0.43 ± 0.012
0.791TrpGly: 0.791 ± 0.012
0.308TrpHis: 0.308 ± 0.009
0.7TrpIle: 0.7 ± 0.014
0.702TrpLys: 0.702 ± 0.014
1.128TrpLeu: 1.128 ± 0.017
0.27TrpMet: 0.27 ± 0.01
0.503TrpAsn: 0.503 ± 0.012
0.524TrpPro: 0.524 ± 0.012
0.484TrpGln: 0.484 ± 0.011
0.891TrpArg: 0.891 ± 0.014
1.14TrpSer: 1.14 ± 0.019
0.915TrpThr: 0.915 ± 0.017
0.833TrpVal: 0.833 ± 0.014
0.265TrpTrp: 0.265 ± 0.008
0.337TrpTyr: 0.337 ± 0.009
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.031TyrAla: 2.031 ± 0.025
0.349TyrCys: 0.349 ± 0.009
1.492TyrAsp: 1.492 ± 0.022
1.231TyrGlu: 1.231 ± 0.017
0.963TyrPhe: 0.963 ± 0.016
1.617TyrGly: 1.617 ± 0.025
0.642TyrHis: 0.642 ± 0.01
1.012TyrIle: 1.012 ± 0.016
0.891TyrLys: 0.891 ± 0.015
2.4TyrLeu: 2.4 ± 0.028
0.368TyrMet: 0.368 ± 0.01
0.779TyrAsn: 0.779 ± 0.015
1.244TyrPro: 1.244 ± 0.018
0.84TyrGln: 0.84 ± 0.013
1.457TyrArg: 1.457 ± 0.02
1.792TyrSer: 1.792 ± 0.021
1.328TyrThr: 1.328 ± 0.019
1.401TyrVal: 1.401 ± 0.019
0.304TyrTrp: 0.304 ± 0.008
0.678TyrTyr: 0.678 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8013 proteins (4386583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski