Enterobacteria phage I2-2 (Bacteriophage I2-2)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Lineavirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P15416|CAPSD_BPI22 Capsid protein G8P OS=Enterobacteria phage I2-2 OX=10869 GN=VIII PE=3 SV=1
MM1 pKa = 7.15KK2 pKa = 10.05RR3 pKa = 11.84KK4 pKa = 9.92IIAISLFLYY13 pKa = 10.26IPLSNADD20 pKa = 3.16NWEE23 pKa = 4.73SITKK27 pKa = 10.09SYY29 pKa = 9.43YY30 pKa = 9.99TGFAMSKK37 pKa = 7.91TVEE40 pKa = 4.45SKK42 pKa = 11.05DD43 pKa = 3.48QDD45 pKa = 3.29GKK47 pKa = 7.68TVRR50 pKa = 11.84KK51 pKa = 9.18EE52 pKa = 4.19VITQADD58 pKa = 4.19LTTACNDD65 pKa = 3.49AKK67 pKa = 11.11ASAQDD72 pKa = 3.35VFNQMKK78 pKa = 9.02LTFSGIWPDD87 pKa = 3.23SQFRR91 pKa = 11.84LVTGDD96 pKa = 3.27TCVYY100 pKa = 10.22NGSPSEE106 pKa = 4.15KK107 pKa = 9.25TEE109 pKa = 3.66SWSIRR114 pKa = 11.84AQVEE118 pKa = 3.58GDD120 pKa = 3.56MQRR123 pKa = 11.84SVPDD127 pKa = 3.88EE128 pKa = 4.42EE129 pKa = 5.3PSEE132 pKa = 4.06QTPEE136 pKa = 4.36EE137 pKa = 3.99ICEE140 pKa = 4.15AKK142 pKa = 10.56PPIDD146 pKa = 3.33GVFNNVSKK154 pKa = 11.05GDD156 pKa = 3.46EE157 pKa = 3.95GGFYY161 pKa = 10.31INYY164 pKa = 9.6NGCEE168 pKa = 3.98YY169 pKa = 10.28EE170 pKa = 4.12ATGVTVCQNDD180 pKa = 3.81GTVCASSAWKK190 pKa = 7.81PTGYY194 pKa = 10.2VPEE197 pKa = 4.89SGEE200 pKa = 4.31SSSSPVKK207 pKa = 11.0DD208 pKa = 3.16GDD210 pKa = 3.69TGGTGEE216 pKa = 4.86GGSDD220 pKa = 2.97TGGDD224 pKa = 3.31TGGGDD229 pKa = 3.38TGGGSTGGDD238 pKa = 3.11TGGSTGGGSTGGGSTGGSTGKK259 pKa = 10.4SLTKK263 pKa = 10.41EE264 pKa = 3.95DD265 pKa = 3.41VTAAIHH271 pKa = 6.46DD272 pKa = 4.4ASPSIGDD279 pKa = 3.67AVKK282 pKa = 10.86DD283 pKa = 4.01SLTEE287 pKa = 5.44DD288 pKa = 3.34NDD290 pKa = 3.85QNDD293 pKa = 3.92NQKK296 pKa = 10.8KK297 pKa = 10.33ADD299 pKa = 3.88EE300 pKa = 4.18QSAKK304 pKa = 10.72ASASVSDD311 pKa = 5.04AISDD315 pKa = 3.64GMRR318 pKa = 11.84GVGNFVDD325 pKa = 4.36DD326 pKa = 4.6LGGEE330 pKa = 4.19SSQYY334 pKa = 11.11GIGNSEE340 pKa = 4.04MDD342 pKa = 4.29LSVSLAKK349 pKa = 10.53GQLGIDD355 pKa = 3.96LEE357 pKa = 4.74GHH359 pKa = 5.62GSAWEE364 pKa = 4.1SFLNDD369 pKa = 3.12GALRR373 pKa = 11.84PSIPSGHH380 pKa = 6.55GCTDD384 pKa = 3.5FVMFQGSVYY393 pKa = 10.49QLDD396 pKa = 3.56IGCDD400 pKa = 3.11KK401 pKa = 11.29LGDD404 pKa = 3.79IKK406 pKa = 11.26SVLSWVMYY414 pKa = 10.54CLTFWYY420 pKa = 9.92VFQSATSLLRR430 pKa = 11.84KK431 pKa = 10.05GEE433 pKa = 3.98QQ434 pKa = 2.97

Molecular weight: 45.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15419-2|REP-2_BPI22 Isoform of P15419 Isoform G10P of Replication-associated protein G2P OS=Enterobacteria phage I2-2 OX=10869 GN=II PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84MTKK5 pKa = 9.62IYY7 pKa = 9.41FKK9 pKa = 11.03KK10 pKa = 9.88MNRR13 pKa = 11.84VWLMASFLALGAGVGFFYY31 pKa = 10.82KK32 pKa = 10.25SRR34 pKa = 11.84QINEE38 pKa = 4.1QLSNMPVASAQANTTKK54 pKa = 10.18TDD56 pKa = 3.22HH57 pKa = 6.95TIDD60 pKa = 3.62EE61 pKa = 4.7LPRR64 pKa = 11.84LSINSFAQMGYY75 pKa = 10.6DD76 pKa = 3.39VNVSFKK82 pKa = 10.36DD83 pKa = 3.53AKK85 pKa = 10.7GKK87 pKa = 10.14IYY89 pKa = 10.84YY90 pKa = 10.49SFDD93 pKa = 3.33LMKK96 pKa = 10.44SGYY99 pKa = 10.73ALDD102 pKa = 4.71IKK104 pKa = 10.56DD105 pKa = 3.62SCHH108 pKa = 4.29ITLRR112 pKa = 11.84KK113 pKa = 9.34RR114 pKa = 11.84NYY116 pKa = 8.15IQQVTCEE123 pKa = 4.06GG124 pKa = 3.45

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

2

10

2047

29

434

204.7

22.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.23 ± 0.752

1.172 ± 0.351

6.155 ± 0.623

3.664 ± 0.698

4.25 ± 0.594

8.647 ± 1.29

1.368 ± 0.329

6.253 ± 0.725

5.716 ± 0.686

7.816 ± 0.694

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.402

4.983 ± 0.6

3.273 ± 0.34

3.908 ± 0.366

4.152 ± 0.687

9.575 ± 0.888

6.351 ± 0.43

8.5 ± 1.053

1.319 ± 0.275

3.224 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski