Flavobacterium sp. phage 1/32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C4H4|A0A088C4H4_9CAUD Uncharacterized protein OS=Flavobacterium sp. phage 1/32 OX=1458858 GN=F132_53 PE=4 SV=1
MM1 pKa = 7.84SYY3 pKa = 11.29DD4 pKa = 3.47DD5 pKa = 4.48WKK7 pKa = 11.12LATPEE12 pKa = 3.92EE13 pKa = 4.38EE14 pKa = 4.71GEE16 pKa = 4.29NEE18 pKa = 4.13CGYY21 pKa = 10.75CGEE24 pKa = 4.14PCEE27 pKa = 5.72KK28 pKa = 9.9EE29 pKa = 4.09FCSRR33 pKa = 11.84EE34 pKa = 4.01CKK36 pKa = 9.84KK37 pKa = 10.85AYY39 pKa = 10.27EE40 pKa = 4.13NDD42 pKa = 3.27

Molecular weight: 4.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C3C1|A0A088C3C1_9CAUD Major capsid protein OS=Flavobacterium sp. phage 1/32 OX=1458858 GN=F132_32 PE=4 SV=1
MM1 pKa = 7.65AGRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 7.73PTKK9 pKa = 9.94RR10 pKa = 11.84EE11 pKa = 3.76KK12 pKa = 11.1LKK14 pKa = 10.92GNSVGFTEE22 pKa = 4.09NRR24 pKa = 11.84EE25 pKa = 4.04NRR27 pKa = 11.84EE28 pKa = 3.77TAMLMLDD35 pKa = 4.66RR36 pKa = 11.84ITKK39 pKa = 8.96QRR41 pKa = 11.84EE42 pKa = 3.59KK43 pKa = 10.86EE44 pKa = 4.27GYY46 pKa = 7.49VWVMKK51 pKa = 9.47GTTKK55 pKa = 10.58KK56 pKa = 10.35QIHH59 pKa = 6.52PDD61 pKa = 3.05KK62 pKa = 11.36VNAHH66 pKa = 6.75LLDD69 pKa = 3.77GWKK72 pKa = 8.44KK73 pKa = 8.76TNNKK77 pKa = 8.8II78 pKa = 3.95

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13677

35

1284

217.1

24.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.528 ± 0.397

0.863 ± 0.143

5.147 ± 0.197

9.827 ± 0.576

4.979 ± 0.191

5.615 ± 0.273

1.155 ± 0.148

7.516 ± 0.24

10.704 ± 0.629

9.344 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.791 ± 0.148

6.449 ± 0.431

2.786 ± 0.2

3.539 ± 0.225

3.744 ± 0.181

4.54 ± 0.183

5.981 ± 0.259

5.813 ± 0.212

1.06 ± 0.134

3.619 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski