Cyanoramphus nest associated circular X DNA virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7UX06|L7UX06_9VIRU Putative capsid protein OS=Cyanoramphus nest associated circular X DNA virus OX=1282443 PE=4 SV=1
MM1 pKa = 7.42NFQRR5 pKa = 11.84KK6 pKa = 7.6RR7 pKa = 11.84WCFTVNNYY15 pKa = 8.82TEE17 pKa = 5.61DD18 pKa = 3.54EE19 pKa = 4.51FNSIKK24 pKa = 10.64QNFCEE29 pKa = 4.14RR30 pKa = 11.84VAKK33 pKa = 9.38FAVMGRR39 pKa = 11.84EE40 pKa = 3.95IGSGGVPHH48 pKa = 6.74LQGFISLQNKK58 pKa = 8.17VRR60 pKa = 11.84LTGIKK65 pKa = 10.22AIVGQRR71 pKa = 11.84AHH73 pKa = 6.62VEE75 pKa = 3.97PAKK78 pKa = 10.45GTDD81 pKa = 3.37QKK83 pKa = 11.66NLEE86 pKa = 4.5YY87 pKa = 9.48CTKK90 pKa = 10.5SCGVDD95 pKa = 2.84GATIYY100 pKa = 10.07GVPAGGVTEE109 pKa = 4.57KK110 pKa = 11.23GGGTPLSRR118 pKa = 11.84RR119 pKa = 11.84ALEE122 pKa = 4.22CVRR125 pKa = 11.84KK126 pKa = 9.92RR127 pKa = 11.84SAGTSISEE135 pKa = 4.65LLDD138 pKa = 4.63DD139 pKa = 4.84DD140 pKa = 4.72TLAPAYY146 pKa = 9.14IVHH149 pKa = 6.28KK150 pKa = 10.49RR151 pKa = 11.84AIEE154 pKa = 3.99EE155 pKa = 4.31CASDD159 pKa = 3.45IAGAQQFQAEE169 pKa = 4.41RR170 pKa = 11.84ARR172 pKa = 11.84YY173 pKa = 7.45ATTRR177 pKa = 11.84WKK179 pKa = 10.24PFQFNILKK187 pKa = 9.78ICMQEE192 pKa = 3.51PHH194 pKa = 6.78PRR196 pKa = 11.84AVHH199 pKa = 5.65WFWDD203 pKa = 3.69AEE205 pKa = 4.35GNTGKK210 pKa = 8.27TFIAKK215 pKa = 10.05YY216 pKa = 10.3LVLLHH221 pKa = 6.8DD222 pKa = 4.37AVRR225 pKa = 11.84FEE227 pKa = 4.45NGKK230 pKa = 9.75SADD233 pKa = 2.76IKK235 pKa = 10.72YY236 pKa = 10.06AYY238 pKa = 9.1KK239 pKa = 10.15GQRR242 pKa = 11.84IVIFDD247 pKa = 3.87FSRR250 pKa = 11.84SQTDD254 pKa = 3.58HH255 pKa = 6.52INYY258 pKa = 9.98EE259 pKa = 3.95VMEE262 pKa = 4.34SVKK265 pKa = 10.85NGIVFSPKK273 pKa = 9.53YY274 pKa = 8.59EE275 pKa = 4.0SGMKK279 pKa = 9.92VFRR282 pKa = 11.84TPHH285 pKa = 6.14MICFANEE292 pKa = 3.92RR293 pKa = 11.84PDD295 pKa = 3.76TSNMSMDD302 pKa = 2.64RR303 pKa = 11.84WEE305 pKa = 3.73IHH307 pKa = 6.99EE308 pKa = 4.04ITTRR312 pKa = 11.84QARR315 pKa = 11.84TPTTLPDD322 pKa = 3.88RR323 pKa = 11.84DD324 pKa = 3.87SDD326 pKa = 3.79THH328 pKa = 6.7EE329 pKa = 4.01IAA331 pKa = 5.06

Molecular weight: 37.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7UX06|L7UX06_9VIRU Putative capsid protein OS=Cyanoramphus nest associated circular X DNA virus OX=1282443 PE=4 SV=1
MM1 pKa = 6.94GRR3 pKa = 11.84KK4 pKa = 8.64RR5 pKa = 11.84AAPWAGKK12 pKa = 10.26SYY14 pKa = 9.92GKK16 pKa = 9.72RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84VIRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84WPVPARR31 pKa = 11.84KK32 pKa = 9.3RR33 pKa = 11.84RR34 pKa = 11.84VPKK37 pKa = 9.83RR38 pKa = 11.84RR39 pKa = 11.84SRR41 pKa = 11.84RR42 pKa = 11.84TRR44 pKa = 11.84KK45 pKa = 5.5TTRR48 pKa = 11.84DD49 pKa = 3.02NARR52 pKa = 11.84VYY54 pKa = 10.57RR55 pKa = 11.84QGLFARR61 pKa = 11.84FIGPTYY67 pKa = 9.8TLSAAANEE75 pKa = 4.24DD76 pKa = 3.08AVYY79 pKa = 9.76TLNFNYY85 pKa = 8.0GTLGQNDD92 pKa = 3.93FAKK95 pKa = 10.61QMEE98 pKa = 5.3GYY100 pKa = 11.16ARR102 pKa = 11.84MFEE105 pKa = 4.24QMKK108 pKa = 10.25CYY110 pKa = 9.64KK111 pKa = 10.66AKK113 pKa = 9.35LTYY116 pKa = 8.9WLNDD120 pKa = 3.08NDD122 pKa = 4.0EE123 pKa = 4.19LAKK126 pKa = 10.72PEE128 pKa = 4.37TKK130 pKa = 8.29WTEE133 pKa = 3.9VVTSYY138 pKa = 11.43DD139 pKa = 3.19PRR141 pKa = 11.84NYY143 pKa = 9.68ARR145 pKa = 11.84NMTFQGALSRR155 pKa = 11.84GNSKK159 pKa = 10.35RR160 pKa = 11.84VILKK164 pKa = 9.72PGRR167 pKa = 11.84KK168 pKa = 7.15YY169 pKa = 9.32TATITPKK176 pKa = 10.02WGVNLAAADD185 pKa = 3.95VPAPSDD191 pKa = 3.44PSVVQVGGRR200 pKa = 11.84SGYY203 pKa = 10.51VNMSRR208 pKa = 11.84VALATDD214 pKa = 3.48ISINSLCMVFHH225 pKa = 6.63GMPAEE230 pKa = 4.11TDD232 pKa = 3.95FLSCYY237 pKa = 8.45NTFYY241 pKa = 11.14CHH243 pKa = 6.92FKK245 pKa = 11.01DD246 pKa = 3.28RR247 pKa = 11.84VQMNPYY253 pKa = 10.04KK254 pKa = 10.74LL255 pKa = 3.56

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

586

255

331

293.0

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.703 ± 0.41

2.048 ± 0.277

4.778 ± 0.268

4.949 ± 1.273

4.608 ± 0.396

6.826 ± 0.319

2.048 ± 0.73

4.778 ± 1.401

6.655 ± 0.233

4.778 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.901 ± 0.363

4.949 ± 0.539

4.608 ± 0.51

3.584 ± 0.485

9.215 ± 1.473

5.29 ± 0.111

6.997 ± 0.036

6.314 ± 0.204

1.706 ± 0.147

4.266 ± 0.934
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski