Microcystis phage Me-ZS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F4X4|A0A3G8F4X4_9CAUD Uncharacterized protein OS=Microcystis phage Me-ZS1 OX=2483660 PE=4 SV=1
MM1 pKa = 7.94KK2 pKa = 10.24YY3 pKa = 9.75RR4 pKa = 11.84YY5 pKa = 9.61AVIDD9 pKa = 4.16LFDD12 pKa = 3.91GTVTGTDD19 pKa = 3.33DD20 pKa = 3.32EE21 pKa = 4.78AQVRR25 pKa = 11.84EE26 pKa = 4.11YY27 pKa = 11.07DD28 pKa = 3.39GSEE31 pKa = 3.79EE32 pKa = 4.08FFVIDD37 pKa = 4.2LQEE40 pKa = 4.39CKK42 pKa = 9.9WIVEE46 pKa = 4.1GVPRR50 pKa = 11.84DD51 pKa = 3.54IPPLFRR57 pKa = 11.84SDD59 pKa = 3.21SS60 pKa = 3.55

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F4R5|A0A3G8F4R5_9CAUD Uncharacterized protein OS=Microcystis phage Me-ZS1 OX=2483660 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84EE3 pKa = 3.59VTGFIRR9 pKa = 11.84ASEE12 pKa = 4.36VVEE15 pKa = 3.82NTAPVRR21 pKa = 11.84GSVSRR26 pKa = 11.84YY27 pKa = 7.44LLCYY31 pKa = 9.5FVQSGKK37 pKa = 9.54PALPMFWTHH46 pKa = 6.04KK47 pKa = 8.01EE48 pKa = 3.55CRR50 pKa = 11.84RR51 pKa = 11.84ALRR54 pKa = 11.84RR55 pKa = 11.84GKK57 pKa = 10.12VNKK60 pKa = 9.79EE61 pKa = 3.61DD62 pKa = 5.08LIPFAGLPSTGQTFMARR79 pKa = 11.84YY80 pKa = 8.83GWLLHH85 pKa = 4.92QTSGIAIGAALALLWTRR102 pKa = 11.84WW103 pKa = 3.24

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15157

38

1813

207.6

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.853 ± 0.722

1.135 ± 0.186

5.337 ± 0.193

6.611 ± 0.314

3.543 ± 0.155

7.805 ± 0.29

1.973 ± 0.256

4.209 ± 0.168

4.071 ± 0.359

8.821 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.106

3.497 ± 0.172

4.988 ± 0.487

3.939 ± 0.329

7.152 ± 0.251

5.793 ± 0.244

6.347 ± 0.267

7.396 ± 0.222

1.517 ± 0.167

2.857 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski