Brevilactibacter sinopodophylli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Brevilactibacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2777 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9KBS2|A0A4Q9KBS2_9ACTN Cobalt transport protein CbiN OS=Brevilactibacter sinopodophylli OX=1837344 GN=cbiN PE=3 SV=1
MM1 pKa = 7.25SADD4 pKa = 3.3AFEE7 pKa = 4.67EE8 pKa = 4.34LVNDD12 pKa = 4.64ALDD15 pKa = 3.86SVPDD19 pKa = 4.16EE20 pKa = 4.54IFALLDD26 pKa = 3.05NCIIVIEE33 pKa = 4.78DD34 pKa = 4.29DD35 pKa = 4.49APADD39 pKa = 4.02DD40 pKa = 4.49PQLLGLYY47 pKa = 9.67EE48 pKa = 5.09GIPLTEE54 pKa = 4.62RR55 pKa = 11.84DD56 pKa = 3.39SHH58 pKa = 7.47YY59 pKa = 11.35GGVLPDD65 pKa = 3.3QIRR68 pKa = 11.84IFRR71 pKa = 11.84NPTLAICDD79 pKa = 3.79TFEE82 pKa = 4.5DD83 pKa = 4.23VVDD86 pKa = 4.35EE87 pKa = 3.99VHH89 pKa = 5.79ITVVHH94 pKa = 6.69EE95 pKa = 4.08IAHH98 pKa = 6.16HH99 pKa = 6.46FGIDD103 pKa = 3.64DD104 pKa = 5.06DD105 pKa = 4.39RR106 pKa = 11.84LHH108 pKa = 7.14DD109 pKa = 4.72LGYY112 pKa = 11.24AA113 pKa = 3.45

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9KGN3|A0A4Q9KGN3_9ACTN Uncharacterized protein OS=Brevilactibacter sinopodophylli OX=1837344 GN=ET989_00145 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 8.44KK23 pKa = 9.1TRR25 pKa = 11.84IQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84AGKK33 pKa = 9.79

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2777

0

2777

911669

24

2060

328.3

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.332 ± 0.071

0.672 ± 0.014

6.194 ± 0.044

5.591 ± 0.04

2.965 ± 0.025

9.046 ± 0.042

2.213 ± 0.026

3.806 ± 0.038

2.097 ± 0.031

10.313 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.96 ± 0.02

2.005 ± 0.025

5.742 ± 0.039

2.827 ± 0.024

7.456 ± 0.052

5.062 ± 0.028

6.059 ± 0.035

9.109 ± 0.039

1.65 ± 0.019

1.901 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski