Leptospirillum ferrodiazotrophum

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Leptospirillum; Leptospirillum sp. Group III

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6HZ00|C6HZ00_9BACT Transposase IS605 OrfB family OS=Leptospirillum ferrodiazotrophum OX=412449 GN=UBAL3_94320042 PE=3 SV=1
MM1 pKa = 7.67ISLFSPFGRR10 pKa = 11.84RR11 pKa = 11.84LSFFSFLLPLVVLASLGGCKK31 pKa = 10.06GGLLAFPGSANEE43 pKa = 4.18SLSGPTLYY51 pKa = 10.65SVSGVAMDD59 pKa = 4.54GPISGGSVAVAQYY72 pKa = 10.84QSDD75 pKa = 3.96GSLVPLGTVTTGRR88 pKa = 11.84DD89 pKa = 3.09GSFQITSLFDD99 pKa = 3.78LSGGPVSFTLTGGTNIDD116 pKa = 3.5IASGATITTPTGVSLTALIPAADD139 pKa = 3.88LTHH142 pKa = 5.88TVVVLTPFSSLEE154 pKa = 3.75ATEE157 pKa = 4.39AQTLASQGSSIEE169 pKa = 4.03EE170 pKa = 4.01ALSKK174 pKa = 10.97ARR176 pKa = 11.84TDD178 pKa = 2.84WSGFLGFDD186 pKa = 3.71PAEE189 pKa = 4.08VPPANMAAGPASANASGIYY208 pKa = 10.3GLLLAGLSQFTNHH221 pKa = 6.88IGQTQNLAPGMANPLGLLPLLEE243 pKa = 4.21QDD245 pKa = 4.67LGDD248 pKa = 3.83GVFNGLAPGNPTPLTYY264 pKa = 10.45YY265 pKa = 10.77GYY267 pKa = 7.88TLSGEE272 pKa = 4.34TLRR275 pKa = 11.84QEE277 pKa = 4.25VTAEE281 pKa = 3.78ALVFLWSGQNQSGLTPQTTDD301 pKa = 3.63GILNGVAMNTGPLFPPSPSPSLPDD325 pKa = 3.93PGTPQVTISSPASATFVNKK344 pKa = 9.46TISVAASATDD354 pKa = 3.4SLGIATLVVTGSGIVLPGGEE374 pKa = 4.16LTGNPVLAEE383 pKa = 3.91IDD385 pKa = 3.85TTKK388 pKa = 10.82SPSGPASLSATATDD402 pKa = 4.02YY403 pKa = 11.68AGNTQTTTTLFTIDD417 pKa = 4.34NVPPTITNLSPANGGLYY434 pKa = 10.65SPGCTGNPTTVTVTGTLQDD453 pKa = 3.88NLSGPSGVQVQEE465 pKa = 4.2TAPSSTSISTTYY477 pKa = 10.97SPVNSTTGNFSFTFIAPANSCKK499 pKa = 8.73TANYY503 pKa = 10.1AFTINGFDD511 pKa = 3.15KK512 pKa = 10.51VTNEE516 pKa = 3.68TVLNYY521 pKa = 10.08TLGVTNN527 pKa = 4.8

Molecular weight: 53.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6HVW0|C6HVW0_9BACT Sigma-54 factor interaction domain-containing protein OS=Leptospirillum ferrodiazotrophum OX=412449 GN=UBAL3_80150010 PE=4 SV=1
MM1 pKa = 7.6SLTFNPSNLRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATTAGRR28 pKa = 11.84KK29 pKa = 7.14VLKK32 pKa = 10.11KK33 pKa = 9.9RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84YY40 pKa = 8.01KK41 pKa = 10.88LSVV44 pKa = 3.04

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2653

0

2653

828293

35

1992

312.2

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.412 ± 0.048

0.781 ± 0.016

4.899 ± 0.028

6.609 ± 0.051

4.132 ± 0.031

8.508 ± 0.059

2.194 ± 0.018

5.247 ± 0.04

4.075 ± 0.05

11.282 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.257 ± 0.02

2.54 ± 0.034

5.948 ± 0.042

2.661 ± 0.024

7.756 ± 0.054

7.231 ± 0.041

5.036 ± 0.044

6.921 ± 0.041

1.214 ± 0.019

2.297 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski