Clostridium colicanis DSM 13634

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium colicanis

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A151ARK3|A0A151ARK3_9CLOT Lipopolysaccharide export system ATP-binding protein LptB OS=Clostridium colicanis DSM 13634 OX=1121305 GN=lptB PE=4 SV=1
MM1 pKa = 7.42QDD3 pKa = 2.97NFEE6 pKa = 4.48VNNNEE11 pKa = 3.81IQQVRR16 pKa = 11.84TDD18 pKa = 3.26EE19 pKa = 4.69CIIAGKK25 pKa = 10.67VYY27 pKa = 10.09GKK29 pKa = 10.18CRR31 pKa = 11.84QQDD34 pKa = 3.73CLRR37 pKa = 11.84PIDD40 pKa = 4.96ANTTPTPGMIQIDD53 pKa = 3.98SSRR56 pKa = 11.84LGEE59 pKa = 4.07TEE61 pKa = 4.37EE62 pKa = 4.25ISGGPLTGTITPNSIISFDD81 pKa = 3.66DD82 pKa = 3.45TVAGVQLVPNTFRR95 pKa = 11.84LSDD98 pKa = 3.4IRR100 pKa = 11.84LQSIQPSIFSQDD112 pKa = 2.36GFYY115 pKa = 11.11DD116 pKa = 3.24VTIRR120 pKa = 11.84YY121 pKa = 6.11TFSYY125 pKa = 10.19QINLLDD131 pKa = 5.26DD132 pKa = 4.34NGDD135 pKa = 4.8PIPVTQGTDD144 pKa = 3.33EE145 pKa = 4.13ITDD148 pKa = 3.26IAAYY152 pKa = 6.0TTYY155 pKa = 10.26TKK157 pKa = 10.13TVSLFGGEE165 pKa = 4.38ASTSTIATLNTLYY178 pKa = 11.02SDD180 pKa = 3.37TASINEE186 pKa = 3.81GNIPYY191 pKa = 10.31VSVQGIANPLSINIGTYY208 pKa = 9.21VVNGDD213 pKa = 3.8TEE215 pKa = 4.11YY216 pKa = 10.97HH217 pKa = 6.38ADD219 pKa = 3.35VTIGLFTIIKK229 pKa = 9.91LFRR232 pKa = 11.84LVNMLVASAGDD243 pKa = 3.75CDD245 pKa = 4.47IPVCEE250 pKa = 4.87PFQTSDD256 pKa = 2.99PCEE259 pKa = 4.17YY260 pKa = 10.4FSSLPFPFDD269 pKa = 4.06DD270 pKa = 5.34FNPPSNVVV278 pKa = 2.89

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151AK94|A0A151AK94_9CLOT UDP-galactopyranose mutase OS=Clostridium colicanis DSM 13634 OX=1121305 GN=glf PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.27KK9 pKa = 7.85RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84MKK23 pKa = 8.7TLSGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2406

0

2406

742156

29

1776

308.5

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.957 ± 0.05

1.13 ± 0.022

5.422 ± 0.044

7.63 ± 0.058

4.196 ± 0.037

6.563 ± 0.048

1.385 ± 0.018

10.187 ± 0.058

9.265 ± 0.056

8.916 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.025

5.953 ± 0.047

2.936 ± 0.026

2.263 ± 0.025

3.609 ± 0.031

6.079 ± 0.037

4.678 ± 0.033

6.572 ± 0.042

0.605 ± 0.015

4.0 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski