Pseudomonas phage uligo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Uliginvirus; Pseudomonas virus uligo

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4P7Q6|A0A2H4P7Q6_9CAUD Putative structural protein OS=Pseudomonas phage uligo OX=2048979 PE=4 SV=1
MM1 pKa = 7.57CNCCPSLLKK10 pKa = 10.14FVYY13 pKa = 10.33AFTDD17 pKa = 3.86RR18 pKa = 11.84YY19 pKa = 8.17TGARR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 7.28QQPADD30 pKa = 4.31AVLQDD35 pKa = 3.97AKK37 pKa = 10.91FSLSVYY43 pKa = 10.02GWATCSRR50 pKa = 11.84CGDD53 pKa = 3.71EE54 pKa = 5.78PEE56 pKa = 6.08DD57 pKa = 3.87DD58 pKa = 5.41LEE60 pKa = 4.19WTTYY64 pKa = 11.35DD65 pKa = 3.13EE66 pKa = 4.71FVSHH70 pKa = 7.18RR71 pKa = 11.84EE72 pKa = 3.98TVEE75 pKa = 4.29DD76 pKa = 4.07PLL78 pKa = 6.0

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4P7M2|A0A2H4P7M2_9CAUD DNA ligase OS=Pseudomonas phage uligo OX=2048979 PE=4 SV=1
MM1 pKa = 7.36SSHH4 pKa = 6.42RR5 pKa = 11.84QRR7 pKa = 11.84EE8 pKa = 3.83HH9 pKa = 7.04SYY11 pKa = 10.72YY12 pKa = 11.14ALGRR16 pKa = 11.84NDD18 pKa = 3.62RR19 pKa = 11.84RR20 pKa = 11.84TGKK23 pKa = 9.68PPRR26 pKa = 11.84PYY28 pKa = 10.01TLPKK32 pKa = 8.11THH34 pKa = 7.43RR35 pKa = 11.84ATYY38 pKa = 8.27MRR40 pKa = 11.84GYY42 pKa = 10.73NSFRR46 pKa = 11.84EE47 pKa = 4.35PEE49 pKa = 4.23PVSKK53 pKa = 10.45PGWGEE58 pKa = 4.35RR59 pKa = 11.84FAFWIARR66 pKa = 11.84KK67 pKa = 9.76LGLSFEE73 pKa = 4.54LRR75 pKa = 11.84GAAA78 pKa = 4.43

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

14841

41

1390

322.6

35.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.285 ± 0.424

0.889 ± 0.143

6.165 ± 0.204

6.314 ± 0.331

3.814 ± 0.267

7.702 ± 0.251

2.277 ± 0.208

4.804 ± 0.211

5.68 ± 0.297

8.045 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.22

4.413 ± 0.266

4.501 ± 0.226

4.541 ± 0.334

5.566 ± 0.238

5.397 ± 0.236

5.99 ± 0.234

6.597 ± 0.301

1.563 ± 0.171

3.254 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski