Velvet bean golden mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1UUE2|A0A1B1UUE2_9GEMI Protein V2 OS=Velvet bean golden mosaic virus OX=1881630 GN=V2 PE=3 SV=1
MM1 pKa = 7.52PHH3 pKa = 6.75RR4 pKa = 11.84ASFRR8 pKa = 11.84LNAKK12 pKa = 9.99NYY14 pKa = 8.46FLTYY18 pKa = 9.35PKK20 pKa = 9.79CTLTKK25 pKa = 10.11EE26 pKa = 4.26CALEE30 pKa = 3.85QILAIEE36 pKa = 4.18TVVNKK41 pKa = 10.3KK42 pKa = 10.46FIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.49LHH51 pKa = 6.58EE52 pKa = 6.12DD53 pKa = 3.79GTPHH57 pKa = 7.12LHH59 pKa = 6.72VLLQFEE65 pKa = 5.61GKK67 pKa = 9.17FQCTNNRR74 pKa = 11.84LFDD77 pKa = 3.24IHH79 pKa = 8.36AEE81 pKa = 4.03TRR83 pKa = 11.84ATRR86 pKa = 11.84FHH88 pKa = 7.08PNIQGAKK95 pKa = 9.24SSSDD99 pKa = 2.97VKK101 pKa = 11.05QYY103 pKa = 9.16MEE105 pKa = 4.61KK106 pKa = 10.84DD107 pKa = 3.4GDD109 pKa = 4.08VLDD112 pKa = 4.86HH113 pKa = 6.64GTFQVDD119 pKa = 3.22GRR121 pKa = 11.84SGRR124 pKa = 11.84GGKK127 pKa = 9.51QSANDD132 pKa = 3.72AYY134 pKa = 10.89AEE136 pKa = 4.76AINAGDD142 pKa = 3.45TAQALNILKK151 pKa = 10.24EE152 pKa = 4.21KK153 pKa = 10.6APKK156 pKa = 10.3DD157 pKa = 3.57YY158 pKa = 11.28VLYY161 pKa = 10.01YY162 pKa = 10.9HH163 pKa = 6.8NLAHH167 pKa = 6.63NLDD170 pKa = 4.47RR171 pKa = 11.84IFGKK175 pKa = 9.57PAEE178 pKa = 4.51LYY180 pKa = 9.3KK181 pKa = 10.75SKK183 pKa = 11.22YY184 pKa = 9.63SLEE187 pKa = 4.19SFDD190 pKa = 5.6NVPCHH195 pKa = 6.07ISSWAEE201 pKa = 3.88KK202 pKa = 9.92NVKK205 pKa = 9.74DD206 pKa = 3.6AAARR210 pKa = 11.84PEE212 pKa = 4.02RR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.11SRR224 pKa = 11.84TGKK227 pKa = 6.05TTWARR232 pKa = 11.84ALGPHH237 pKa = 6.7NYY239 pKa = 10.19LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.63WQSNVKK286 pKa = 9.81YY287 pKa = 10.5GKK289 pKa = 7.69PTQIKK294 pKa = 10.11GGIPTIFLCNQGPKK308 pKa = 9.95SSYY311 pKa = 10.79KK312 pKa = 10.33EE313 pKa = 3.86FLEE316 pKa = 4.34EE317 pKa = 3.83EE318 pKa = 4.27QNASLKK324 pKa = 10.25VWALKK329 pKa = 10.26NAEE332 pKa = 4.44FYY334 pKa = 10.19TIKK337 pKa = 10.87APFFSSIDD345 pKa = 3.38QSATQVRR352 pKa = 11.84QEE354 pKa = 4.52EE355 pKa = 4.79SNSTQTNN362 pKa = 3.24

Molecular weight: 41.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1UUG4|A0A1B1UUG4_9GEMI Replication enhancer OS=Velvet bean golden mosaic virus OX=1881630 GN=C3 PE=3 SV=1
MM1 pKa = 8.06PKK3 pKa = 10.14RR4 pKa = 11.84NYY6 pKa = 8.53DD7 pKa = 3.68TAFSTPLSATRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.25TPMALPAPASNAQGTTRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WTYY47 pKa = 9.75RR48 pKa = 11.84PMWRR52 pKa = 11.84KK53 pKa = 8.46PRR55 pKa = 11.84FYY57 pKa = 10.74RR58 pKa = 11.84LYY60 pKa = 10.21RR61 pKa = 11.84SPDD64 pKa = 3.2VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 10.21VQSFEE79 pKa = 3.84QRR81 pKa = 11.84HH82 pKa = 6.27DD83 pKa = 3.18IAHH86 pKa = 6.21TGKK89 pKa = 9.87VLCISDD95 pKa = 3.56VTRR98 pKa = 11.84GNGITHH104 pKa = 6.78RR105 pKa = 11.84VGKK108 pKa = 9.62RR109 pKa = 11.84FCIKK113 pKa = 10.16SVYY116 pKa = 8.78ITGKK120 pKa = 9.34VWMDD124 pKa = 3.29EE125 pKa = 4.15NIKK128 pKa = 10.71SKK130 pKa = 10.61NHH132 pKa = 5.84TNTVMFKK139 pKa = 10.36LVRR142 pKa = 11.84DD143 pKa = 3.79RR144 pKa = 11.84RR145 pKa = 11.84PFGTPMDD152 pKa = 4.58FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.81NDD172 pKa = 2.84LRR174 pKa = 11.84DD175 pKa = 3.41RR176 pKa = 11.84YY177 pKa = 9.96QVLRR181 pKa = 11.84RR182 pKa = 11.84FNSTVTGGQYY192 pKa = 10.62ACKK195 pKa = 8.94EE196 pKa = 3.74QAMVNRR202 pKa = 11.84FFRR205 pKa = 11.84VNNHH209 pKa = 4.37VVYY212 pKa = 10.63NHH214 pKa = 5.84QEE216 pKa = 3.45AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.63FYY252 pKa = 11.33DD253 pKa = 3.78SIGNN257 pKa = 3.69

Molecular weight: 30.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1103

97

362

183.8

21.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 1.167

2.448 ± 0.393

4.896 ± 0.675

4.986 ± 0.681

4.533 ± 0.294

4.805 ± 0.456

3.989 ± 0.522

5.44 ± 0.568

6.618 ± 0.709

7.978 ± 1.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 1.025

6.256 ± 0.368

4.805 ± 0.439

4.17 ± 0.507

7.162 ± 1.18

7.162 ± 0.884

6.256 ± 0.612

4.533 ± 0.734

1.179 ± 0.107

4.352 ± 0.718
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski