Prevotella sp. CAG:279

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7HR13|R7HR13_9BACT Uncharacterized protein OS=Prevotella sp. CAG:279 OX=1262924 GN=BN585_00723 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.5KK3 pKa = 10.85YY4 pKa = 11.05ICTVCDD10 pKa = 3.11WVYY13 pKa = 11.18DD14 pKa = 4.0PEE16 pKa = 6.07IGDD19 pKa = 4.19PEE21 pKa = 4.96HH22 pKa = 7.12GIAPGTKK29 pKa = 9.81FEE31 pKa = 6.11DD32 pKa = 4.08IPDD35 pKa = 3.66DD36 pKa = 4.07WVCPLCGVGKK46 pKa = 10.53EE47 pKa = 4.11DD48 pKa = 5.13FEE50 pKa = 6.84KK51 pKa = 10.4IDD53 pKa = 3.3

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7HN07|R7HN07_9BACT C-terminal processing peptidase OS=Prevotella sp. CAG:279 OX=1262924 GN=BN585_01673 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.36KK6 pKa = 9.96RR7 pKa = 11.84KK8 pKa = 6.44GHH10 pKa = 6.2KK11 pKa = 9.14MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2076

0

2076

709329

29

2467

341.7

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.416 ± 0.063

1.323 ± 0.02

6.082 ± 0.042

6.014 ± 0.045

4.458 ± 0.036

7.041 ± 0.045

1.823 ± 0.028

6.436 ± 0.049

6.179 ± 0.052

8.559 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.028

4.835 ± 0.045

3.631 ± 0.026

3.034 ± 0.029

4.968 ± 0.048

6.3 ± 0.046

5.65 ± 0.044

7.311 ± 0.042

1.105 ± 0.019

4.027 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski