Hubei toti-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF57|A0A1L3KF57_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 12 OX=1923300 PE=4 SV=1
MM1 pKa = 7.51PADD4 pKa = 4.05LRR6 pKa = 11.84DD7 pKa = 3.78YY8 pKa = 10.06MCNCSSVAQTTGAHH22 pKa = 6.45ASLSLHH28 pKa = 6.28HH29 pKa = 6.57SVSRR33 pKa = 11.84LQVFAIDD40 pKa = 3.79VPGGEE45 pKa = 4.26VGIEE49 pKa = 3.76GGVNYY54 pKa = 10.6SIMRR58 pKa = 11.84RR59 pKa = 11.84IMPSTGLGCHH69 pKa = 5.81QGSPWVTSTFNEE81 pKa = 4.21VLQKK85 pKa = 10.62LVAPLEE91 pKa = 4.5STSGNYY97 pKa = 8.87GALVSNPAYY106 pKa = 10.85SDD108 pKa = 3.88FGSLAYY114 pKa = 9.41LTDD117 pKa = 3.64VLAKK121 pKa = 9.84STGSWLDD128 pKa = 3.04EE129 pKa = 4.35SPIVRR134 pKa = 11.84CLLLGDD140 pKa = 4.59LSVSHH145 pKa = 6.5RR146 pKa = 11.84TLQCDD151 pKa = 3.44PLIGQIVSDD160 pKa = 3.96GQIKK164 pKa = 9.93RR165 pKa = 11.84VEE167 pKa = 4.37RR168 pKa = 11.84YY169 pKa = 9.08KK170 pKa = 10.0PTYY173 pKa = 9.09PRR175 pKa = 11.84PDD177 pKa = 3.65DD178 pKa = 4.11ALNPAEE184 pKa = 4.19VQAMAVAMDD193 pKa = 3.74SWVMLRR199 pKa = 11.84TGQRR203 pKa = 11.84NVPDD207 pKa = 3.91WNVHH211 pKa = 4.78DD212 pKa = 4.12WSYY215 pKa = 11.86SVAIVPCEE223 pKa = 3.62TSMLEE228 pKa = 4.39SNSIWLYY235 pKa = 8.53MASFLTTEE243 pKa = 3.86IWNNRR248 pKa = 11.84YY249 pKa = 7.0VWKK252 pKa = 10.66VHH254 pKa = 4.48VQPRR258 pKa = 11.84SDD260 pKa = 3.26MSSEE264 pKa = 4.18EE265 pKa = 4.2PEE267 pKa = 4.04HH268 pKa = 7.34EE269 pKa = 4.26YY270 pKa = 10.77MLMVRR275 pKa = 11.84PRR277 pKa = 11.84AAQVEE282 pKa = 4.57VQGPGRR288 pKa = 11.84VMLVVTNITSRR299 pKa = 11.84SNLRR303 pKa = 11.84WVTIDD308 pKa = 3.67GFNIPVWNPASGIGPQSVDD327 pKa = 3.08IMPMLLQYY335 pKa = 11.27LDD337 pKa = 3.92LQGATNNISQNWLQAWKK354 pKa = 9.54WMCDD358 pKa = 2.99RR359 pKa = 11.84VSSGNALNHH368 pKa = 6.32ALFLAAEE375 pKa = 4.66TSRR378 pKa = 11.84VQHH381 pKa = 6.49IAPLAHH387 pKa = 6.52PAVEE391 pKa = 4.0NSMPFYY397 pKa = 10.71SINLRR402 pKa = 11.84KK403 pKa = 9.4EE404 pKa = 3.76LPRR407 pKa = 11.84ILKK410 pKa = 10.21SNWEE414 pKa = 3.86MRR416 pKa = 11.84NWADD420 pKa = 4.29VDD422 pKa = 3.79PDD424 pKa = 3.82PEE426 pKa = 4.27KK427 pKa = 10.79EE428 pKa = 3.8KK429 pKa = 11.19SRR431 pKa = 11.84IHH433 pKa = 6.93DD434 pKa = 4.11WIEE437 pKa = 3.39QSIAAWISPLCIHH450 pKa = 7.15SNLTCSFEE458 pKa = 4.43TAEE461 pKa = 4.23SPDD464 pKa = 3.27WSKK467 pKa = 11.49LDD469 pKa = 3.49WMRR472 pKa = 11.84HH473 pKa = 3.89KK474 pKa = 10.45HH475 pKa = 6.0GADD478 pKa = 3.1QTATCHH484 pKa = 5.93EE485 pKa = 4.47ATPDD489 pKa = 3.25LRR491 pKa = 11.84VARR494 pKa = 11.84FVGLIAVPTPIVYY507 pKa = 9.56IDD509 pKa = 3.58PVAVQEE515 pKa = 4.42SVSAIAGMQVAGCAWMQMVTGISWLRR541 pKa = 11.84WVLYY545 pKa = 8.93TAAQNDD551 pKa = 3.96ATRR554 pKa = 11.84MVIQDD559 pKa = 3.11RR560 pKa = 11.84MMNEE564 pKa = 3.56ATAHH568 pKa = 5.87HH569 pKa = 6.7VSSGRR574 pKa = 11.84PDD576 pKa = 3.32KK577 pKa = 10.95TWINLLDD584 pKa = 3.77KK585 pKa = 11.25VLGVQVKK592 pKa = 10.04FDD594 pKa = 4.07FPQLLKK600 pKa = 9.68NTPSVLPAWWQVKK613 pKa = 9.77FILQKK618 pKa = 10.17MNYY621 pKa = 9.04LMPGEE626 pKa = 4.15LEE628 pKa = 3.85RR629 pKa = 11.84RR630 pKa = 11.84QRR632 pKa = 11.84PVFKK636 pKa = 10.78LGVLEE641 pKa = 4.68SSMKK645 pKa = 10.39VGFYY649 pKa = 11.07ANDD652 pKa = 3.56SKK654 pKa = 11.01HH655 pKa = 4.33WQAVARR661 pKa = 11.84YY662 pKa = 7.13TSMLGVSEE670 pKa = 4.5TNDD673 pKa = 3.25VPLYY677 pKa = 10.23AANNEE682 pKa = 4.32MFSHH686 pKa = 6.7LTQSYY691 pKa = 9.58LQLNLTRR698 pKa = 11.84LFTMCAEE705 pKa = 4.34SVYY708 pKa = 10.42HH709 pKa = 7.28SNGTYY714 pKa = 10.09YY715 pKa = 11.09NPLTVVDD722 pKa = 4.39TACAITPVANSLHH735 pKa = 5.61TSIPAEE741 pKa = 3.8LLIIKK746 pKa = 7.91TKK748 pKa = 10.79AGAGTNVGDD757 pKa = 3.73QTVLGEE763 pKa = 4.68WPDD766 pKa = 3.86PWWDD770 pKa = 3.04WLLAGVRR777 pKa = 11.84AAIPFLGSSIPTAALVGAASGLHH800 pKa = 5.88SFLAEE805 pKa = 3.84KK806 pKa = 10.54LNPSRR811 pKa = 11.84AKK813 pKa = 9.81EE814 pKa = 3.85EE815 pKa = 3.86QMIARR820 pKa = 11.84AQATKK825 pKa = 10.56ALEE828 pKa = 4.16SSAEE832 pKa = 3.94AVHH835 pKa = 7.09DD836 pKa = 3.84VLSTGRR842 pKa = 11.84EE843 pKa = 3.72AVGRR847 pKa = 11.84ISKK850 pKa = 10.31AINDD854 pKa = 3.9FTSDD858 pKa = 3.54QLDD861 pKa = 3.68EE862 pKa = 4.26

Molecular weight: 95.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF57|A0A1L3KF57_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 12 OX=1923300 PE=4 SV=1
MM1 pKa = 7.22ARR3 pKa = 11.84SMVGLAVGWSACCNTLFGFKK23 pKa = 10.15HH24 pKa = 6.2PNGCTCRR31 pKa = 11.84CCEE34 pKa = 3.58WTALFFSGEE43 pKa = 4.12AKK45 pKa = 10.23PKK47 pKa = 8.02PCKK50 pKa = 10.02RR51 pKa = 11.84GANDD55 pKa = 3.33RR56 pKa = 11.84TGTSHH61 pKa = 6.9EE62 pKa = 4.43SAGVEE67 pKa = 4.12CGSCARR73 pKa = 11.84CVIDD77 pKa = 3.43RR78 pKa = 11.84TRR80 pKa = 11.84SSGTYY85 pKa = 8.91IKK87 pKa = 10.71SNQRR91 pKa = 11.84FYY93 pKa = 11.27EE94 pKa = 4.05RR95 pKa = 11.84STRR98 pKa = 11.84RR99 pKa = 11.84VTVSDD104 pKa = 3.79AQLPLYY110 pKa = 9.02WDD112 pKa = 3.47PGYY115 pKa = 9.91WVQAINLLQPASSSIEE131 pKa = 3.83FHH133 pKa = 7.77DD134 pKa = 4.78DD135 pKa = 3.23LSLHH139 pKa = 6.22RR140 pKa = 11.84SRR142 pKa = 11.84YY143 pKa = 8.93KK144 pKa = 9.57IHH146 pKa = 6.5SAPGVGVYY154 pKa = 9.83SRR156 pKa = 11.84PMSFYY161 pKa = 11.0LDD163 pKa = 3.2TLAQLEE169 pKa = 4.62VGCGIGSPYY178 pKa = 9.41WVLAVLLQRR187 pKa = 11.84ARR189 pKa = 11.84AAQDD193 pKa = 3.63PQLSCYY199 pKa = 8.94QWLKK203 pKa = 11.04GLTEE207 pKa = 5.11HH208 pKa = 7.11IEE210 pKa = 4.21TMDD213 pKa = 3.33GDD215 pKa = 4.06RR216 pKa = 11.84FVCSQILVGLEE227 pKa = 3.47RR228 pKa = 11.84VTPPKK233 pKa = 10.11QWSKK237 pKa = 11.91DD238 pKa = 3.62DD239 pKa = 5.07LIADD243 pKa = 4.39AKK245 pKa = 10.91NWVGPNKK252 pKa = 9.08TWASSRR258 pKa = 11.84GVRR261 pKa = 11.84EE262 pKa = 3.95WVEE265 pKa = 3.6GWVEE269 pKa = 3.94KK270 pKa = 10.81KK271 pKa = 9.29FAKK274 pKa = 8.62WQRR277 pKa = 11.84TMDD280 pKa = 3.59MIGRR284 pKa = 11.84EE285 pKa = 3.91NLSFEE290 pKa = 4.92EE291 pKa = 4.66FVQDD295 pKa = 3.38PTRR298 pKa = 11.84WATSGGAPRR307 pKa = 11.84VEE309 pKa = 4.21WEE311 pKa = 3.68GDD313 pKa = 3.19EE314 pKa = 5.08YY315 pKa = 10.76RR316 pKa = 11.84SKK318 pKa = 10.26WAWAMKK324 pKa = 10.07NISEE328 pKa = 4.25GKK330 pKa = 9.79YY331 pKa = 10.38LYY333 pKa = 10.66AQAKK337 pKa = 6.26TMPNIAHH344 pKa = 6.97AAIKK348 pKa = 9.96EE349 pKa = 4.32EE350 pKa = 4.1PSKK353 pKa = 10.12TRR355 pKa = 11.84LVITTPMSSYY365 pKa = 10.67LRR367 pKa = 11.84QSYY370 pKa = 10.05VMYY373 pKa = 10.77LFGIPEE379 pKa = 4.83LNSPIYY385 pKa = 10.62GNKK388 pKa = 9.15QLGEE392 pKa = 4.16LNNVKK397 pKa = 9.21WASYY401 pKa = 10.65CSIDD405 pKa = 4.58AKK407 pKa = 11.17AFDD410 pKa = 4.02HH411 pKa = 6.82QIPIWFIKK419 pKa = 10.53LVMEE423 pKa = 4.7HH424 pKa = 6.83LFKK427 pKa = 11.14SVGRR431 pKa = 11.84EE432 pKa = 3.81DD433 pKa = 2.9LWYY436 pKa = 10.37EE437 pKa = 3.75EE438 pKa = 4.12RR439 pKa = 11.84AHH441 pKa = 6.71INRR444 pKa = 11.84LKK446 pKa = 10.52IEE448 pKa = 4.19VFGEE452 pKa = 3.96IIPYY456 pKa = 9.82HH457 pKa = 6.22GGVLSGWRR465 pKa = 11.84LTSLLGTLASQALCEE480 pKa = 3.99WLILQGVNTNFVVQGDD496 pKa = 4.53DD497 pKa = 2.95ILLYY501 pKa = 10.19SAEE504 pKa = 4.85PIPPNVPDD512 pKa = 3.62MVQEE516 pKa = 4.61FGLKK520 pKa = 9.1IDD522 pKa = 4.16PVVSIPRR529 pKa = 11.84TCGYY533 pKa = 9.78FLRR536 pKa = 11.84RR537 pKa = 11.84AYY539 pKa = 10.25GGSFNLMSSGRR550 pKa = 11.84ALRR553 pKa = 11.84SLFYY557 pKa = 10.62ASPWVEE563 pKa = 3.21RR564 pKa = 11.84SQFQTPNSLARR575 pKa = 11.84SWLQFMSRR583 pKa = 11.84VPGKK587 pKa = 8.93SARR590 pKa = 11.84PWLLRR595 pKa = 11.84QAAADD600 pKa = 3.67MARR603 pKa = 11.84WARR606 pKa = 11.84WPGWTKK612 pKa = 10.65NRR614 pKa = 11.84WFEE617 pKa = 5.19LLTTDD622 pKa = 4.94AGLGGLGTVDD632 pKa = 2.68THH634 pKa = 7.76IYY636 pKa = 9.5RR637 pKa = 11.84QYY639 pKa = 11.02LPQIKK644 pKa = 10.0EE645 pKa = 4.07DD646 pKa = 3.44IDD648 pKa = 3.88RR649 pKa = 11.84SRR651 pKa = 11.84HH652 pKa = 5.87ARR654 pKa = 11.84TAWKK658 pKa = 9.96RR659 pKa = 11.84LYY661 pKa = 10.28EE662 pKa = 4.07YY663 pKa = 9.82FVPVSGLMAAKK674 pKa = 9.72DD675 pKa = 3.44QQVFYY680 pKa = 10.75RR681 pKa = 11.84MSYY684 pKa = 9.41RR685 pKa = 11.84PYY687 pKa = 10.57VRR689 pKa = 11.84PTPPTSAPKK698 pKa = 10.38LATDD702 pKa = 3.37WADD705 pKa = 3.42GVNKK709 pKa = 10.02TDD711 pKa = 2.9MCFNVMQGGFKK722 pKa = 10.72ALPEE726 pKa = 4.1DD727 pKa = 4.05VKK729 pKa = 11.02RR730 pKa = 11.84SAPHH734 pKa = 6.32WLRR737 pKa = 11.84TQPWFRR743 pKa = 11.84VLDD746 pKa = 3.45WLLRR750 pKa = 11.84PEE752 pKa = 4.72EE753 pKa = 4.1MTAPQHH759 pKa = 6.73LCIQPWVLNEE769 pKa = 4.04MLQSDD774 pKa = 4.0ISAADD779 pKa = 3.56RR780 pKa = 11.84VLHH783 pKa = 5.47NMKK786 pKa = 10.36KK787 pKa = 10.45GVISKK792 pKa = 9.96KK793 pKa = 9.33FSMYY797 pKa = 9.73QWILARR803 pKa = 11.84AGKK806 pKa = 10.54LMVALGSWW814 pKa = 3.25

Molecular weight: 93.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1676

814

862

838.0

94.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.413 ± 0.472

1.85 ± 0.254

4.714 ± 0.204

4.952 ± 0.059

2.924 ± 0.534

6.086 ± 0.557

2.387 ± 0.295

4.654 ± 0.162

4.057 ± 0.687

9.01 ± 0.115

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.341 ± 0.275

3.938 ± 0.608

5.668 ± 0.012

4.296 ± 0.003

5.907 ± 0.854

8.234 ± 0.433

5.131 ± 0.325

7.279 ± 0.797

3.938 ± 0.34

3.222 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski