Elioraea sp. Yellowstone

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Elioraeaceae; Elioraea; unclassified Elioraea

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A541C129|A0A541C129_9PROT Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B OS=Elioraea sp. Yellowstone OX=2592070 GN=gatB PE=3 SV=1
MM1 pKa = 6.9TALRR5 pKa = 11.84TMTATLALMGGLALAPAAEE24 pKa = 4.49AAPSYY29 pKa = 9.62GAPAGWTGIGTVGTGTPNGDD49 pKa = 3.24VGTIPAGHH57 pKa = 5.5TQYY60 pKa = 11.66LFVTTDD66 pKa = 2.83EE67 pKa = 4.68GADD70 pKa = 3.39GGGNLLSLGDD80 pKa = 3.9EE81 pKa = 4.81TNGSTLTSPLFAANAGDD98 pKa = 3.6ALEE101 pKa = 5.48FYY103 pKa = 10.67FNYY106 pKa = 8.45VTSDD110 pKa = 3.09GAGFADD116 pKa = 4.41YY117 pKa = 10.97AWARR121 pKa = 11.84LLDD124 pKa = 3.84ASGDD128 pKa = 3.77EE129 pKa = 4.31VALLFTARR137 pKa = 11.84TVPAPDD143 pKa = 4.08NIVPGTGMPSPDD155 pKa = 3.47ATLDD159 pKa = 3.68PATVPIVLGTGNDD172 pKa = 3.99GGPVWDD178 pKa = 4.64EE179 pKa = 4.4LGSDD183 pKa = 3.82SGSCFDD189 pKa = 5.48DD190 pKa = 4.34GCGLSGWVKK199 pKa = 10.05SSYY202 pKa = 10.43TIVTAGTYY210 pKa = 9.91RR211 pKa = 11.84LQFGVTNWGDD221 pKa = 3.13TAYY224 pKa = 10.86QSGLAIAGATIAGDD238 pKa = 4.42PIDD241 pKa = 4.6PQPVPEE247 pKa = 4.51PASLALFGLGLLGLGLAARR266 pKa = 11.84RR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84AA270 pKa = 2.98

Molecular weight: 27.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A541BV78|A0A541BV78_9PROT TPP_enzyme_M domain-containing protein (Fragment) OS=Elioraea sp. Yellowstone OX=2592070 GN=FK498_19060 PE=4 SV=1
PP1 pKa = 7.65RR2 pKa = 11.84PGAGAAQPGADD13 pKa = 3.62PQRR16 pKa = 11.84RR17 pKa = 11.84PRR19 pKa = 11.84GAAPAGLGHH28 pKa = 6.51RR29 pKa = 11.84RR30 pKa = 11.84ARR32 pKa = 11.84GAGLHH37 pKa = 6.08RR38 pKa = 11.84RR39 pKa = 11.84LSARR43 pKa = 11.84RR44 pKa = 11.84ARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84GLPDD52 pKa = 3.09PRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84PLRR59 pKa = 11.84PARR62 pKa = 11.84VHH64 pKa = 6.72AGGARR69 pKa = 11.84LRR71 pKa = 4.01

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3683

0

3683

1151309

24

1856

312.6

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.049 ± 0.081

0.834 ± 0.012

4.915 ± 0.031

5.539 ± 0.034

3.317 ± 0.023

9.102 ± 0.038

1.981 ± 0.019

4.399 ± 0.027

1.756 ± 0.028

10.851 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.021

1.778 ± 0.021

6.177 ± 0.035

2.399 ± 0.022

9.008 ± 0.037

3.745 ± 0.019

4.926 ± 0.026

7.821 ± 0.033

1.479 ± 0.016

1.723 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski