Amino acid dipepetide frequency for Thermosphaera aggregans (strain DSM 11486 / M11TL)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.138AlaAla: 4.138 ± 0.126
0.604AlaCys: 0.604 ± 0.038
2.467AlaAsp: 2.467 ± 0.078
4.48AlaGlu: 4.48 ± 0.109
2.897AlaPhe: 2.897 ± 0.095
5.324AlaGly: 5.324 ± 0.136
1.082AlaHis: 1.082 ± 0.05
4.594AlaIle: 4.594 ± 0.109
3.771AlaLys: 3.771 ± 0.118
8.629AlaLeu: 8.629 ± 0.141
1.51AlaMet: 1.51 ± 0.064
1.639AlaAsn: 1.639 ± 0.059
2.278AlaPro: 2.278 ± 0.076
1.266AlaGln: 1.266 ± 0.062
4.483AlaArg: 4.483 ± 0.108
5.316AlaSer: 5.316 ± 0.141
3.31AlaThr: 3.31 ± 0.105
6.303AlaVal: 6.303 ± 0.141
0.906AlaTrp: 0.906 ± 0.052
2.978AlaTyr: 2.978 ± 0.095
0.0AlaXaa: 0.0 ± 0.0
Cys
0.337CysAla: 0.337 ± 0.033
0.07CysCys: 0.07 ± 0.013
0.305CysAsp: 0.305 ± 0.03
0.438CysGlu: 0.438 ± 0.033
0.35CysPhe: 0.35 ± 0.028
0.863CysGly: 0.863 ± 0.052
0.101CysHis: 0.101 ± 0.017
0.425CysIle: 0.425 ± 0.03
0.415CysLys: 0.415 ± 0.033
0.644CysLeu: 0.644 ± 0.042
0.166CysMet: 0.166 ± 0.021
0.279CysAsn: 0.279 ± 0.029
0.634CysPro: 0.634 ± 0.046
0.126CysGln: 0.126 ± 0.019
0.566CysArg: 0.566 ± 0.038
0.529CysSer: 0.529 ± 0.04
0.33CysThr: 0.33 ± 0.03
0.579CysVal: 0.579 ± 0.039
0.101CysTrp: 0.101 ± 0.016
0.249CysTyr: 0.249 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.0AspAla: 3.0 ± 0.077
0.277AspCys: 0.277 ± 0.028
1.624AspAsp: 1.624 ± 0.059
3.65AspGlu: 3.65 ± 0.112
1.946AspPhe: 1.946 ± 0.074
2.537AspGly: 2.537 ± 0.088
0.71AspHis: 0.71 ± 0.044
3.116AspIle: 3.116 ± 0.102
2.796AspLys: 2.796 ± 0.09
4.767AspLeu: 4.767 ± 0.117
0.901AspMet: 0.901 ± 0.049
1.266AspAsn: 1.266 ± 0.062
2.683AspPro: 2.683 ± 0.078
0.687AspGln: 0.687 ± 0.039
2.077AspArg: 2.077 ± 0.08
2.369AspSer: 2.369 ± 0.072
1.634AspThr: 1.634 ± 0.069
4.168AspVal: 4.168 ± 0.085
0.534AspTrp: 0.534 ± 0.042
2.311AspTyr: 2.311 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
6.142GluAla: 6.142 ± 0.146
0.554GluCys: 0.554 ± 0.032
3.018GluAsp: 3.018 ± 0.088
6.721GluGlu: 6.721 ± 0.162
2.447GluPhe: 2.447 ± 0.078
4.297GluGly: 4.297 ± 0.119
1.216GluHis: 1.216 ± 0.058
5.215GluIle: 5.215 ± 0.129
6.884GluLys: 6.884 ± 0.151
7.823GluLeu: 7.823 ± 0.151
1.503GluMet: 1.503 ± 0.059
2.819GluAsn: 2.819 ± 0.084
2.575GluPro: 2.575 ± 0.089
1.254GluGln: 1.254 ± 0.059
3.796GluArg: 3.796 ± 0.127
3.582GluSer: 3.582 ± 0.101
3.479GluThr: 3.479 ± 0.089
6.295GluVal: 6.295 ± 0.121
0.876GluTrp: 0.876 ± 0.048
3.202GluTyr: 3.202 ± 0.088
0.0GluXaa: 0.0 ± 0.0
Phe
2.346PheAla: 2.346 ± 0.084
0.3PheCys: 0.3 ± 0.024
1.666PheAsp: 1.666 ± 0.073
2.799PheGlu: 2.799 ± 0.087
1.634PhePhe: 1.634 ± 0.069
1.835PheGly: 1.835 ± 0.067
0.697PheHis: 0.697 ± 0.041
3.29PheIle: 3.29 ± 0.102
3.234PheLys: 3.234 ± 0.091
4.098PheLeu: 4.098 ± 0.116
0.949PheMet: 0.949 ± 0.05
1.994PheAsn: 1.994 ± 0.068
1.405PhePro: 1.405 ± 0.058
0.904PheGln: 0.904 ± 0.041
1.782PheArg: 1.782 ± 0.066
2.739PheSer: 2.739 ± 0.088
2.882PheThr: 2.882 ± 0.082
2.316PheVal: 2.316 ± 0.08
0.327PheTrp: 0.327 ± 0.029
1.782PheTyr: 1.782 ± 0.07
0.0PheXaa: 0.0 ± 0.0
Gly
4.037GlyAla: 4.037 ± 0.13
0.687GlyCys: 0.687 ± 0.043
3.396GlyAsp: 3.396 ± 0.095
5.517GlyGlu: 5.517 ± 0.135
4.546GlyPhe: 4.546 ± 0.124
5.309GlyGly: 5.309 ± 0.144
0.999GlyHis: 0.999 ± 0.05
4.858GlyIle: 4.858 ± 0.101
4.412GlyLys: 4.412 ± 0.101
8.888GlyLeu: 8.888 ± 0.16
1.533GlyMet: 1.533 ± 0.056
2.132GlyAsn: 2.132 ± 0.085
1.921GlyPro: 1.921 ± 0.082
1.022GlyGln: 1.022 ± 0.056
4.543GlyArg: 4.543 ± 0.114
4.284GlySer: 4.284 ± 0.108
2.52GlyThr: 2.52 ± 0.09
7.717GlyVal: 7.717 ± 0.155
1.035GlyTrp: 1.035 ± 0.05
3.104GlyTyr: 3.104 ± 0.085
0.0GlyXaa: 0.0 ± 0.0
His
1.198HisAla: 1.198 ± 0.051
0.154HisCys: 0.154 ± 0.019
0.667HisAsp: 0.667 ± 0.038
1.125HisGlu: 1.125 ± 0.054
0.531HisPhe: 0.531 ± 0.039
1.616HisGly: 1.616 ± 0.065
0.35HisHis: 0.35 ± 0.033
0.987HisIle: 0.987 ± 0.054
0.722HisLys: 0.722 ± 0.043
1.676HisLeu: 1.676 ± 0.066
0.385HisMet: 0.385 ± 0.033
0.41HisAsn: 0.41 ± 0.03
1.193HisPro: 1.193 ± 0.054
0.34HisGln: 0.34 ± 0.029
0.934HisArg: 0.934 ± 0.046
0.878HisSer: 0.878 ± 0.045
0.654HisThr: 0.654 ± 0.046
1.624HisVal: 1.624 ± 0.057
0.214HisTrp: 0.214 ± 0.022
0.715HisTyr: 0.715 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.184IleAla: 6.184 ± 0.134
0.415IleCys: 0.415 ± 0.032
3.783IleAsp: 3.783 ± 0.104
5.173IleGlu: 5.173 ± 0.137
2.109IlePhe: 2.109 ± 0.075
5.709IleGly: 5.709 ± 0.131
1.321IleHis: 1.321 ± 0.059
6.336IleIle: 6.336 ± 0.136
3.408IleLys: 3.408 ± 0.101
8.15IleLeu: 8.15 ± 0.138
1.553IleMet: 1.553 ± 0.054
2.235IleAsn: 2.235 ± 0.075
4.445IlePro: 4.445 ± 0.101
1.384IleGln: 1.384 ± 0.057
3.436IleArg: 3.436 ± 0.089
4.579IleSer: 4.579 ± 0.105
3.891IleThr: 3.891 ± 0.106
7.413IleVal: 7.413 ± 0.133
0.649IleTrp: 0.649 ± 0.044
2.887IleTyr: 2.887 ± 0.089
0.0IleXaa: 0.0 ± 0.0
Lys
4.677LysAla: 4.677 ± 0.118
0.68LysCys: 0.68 ± 0.048
2.442LysAsp: 2.442 ± 0.066
4.518LysGlu: 4.518 ± 0.106
1.306LysPhe: 1.306 ± 0.061
4.566LysGly: 4.566 ± 0.114
1.377LysHis: 1.377 ± 0.069
5.601LysIle: 5.601 ± 0.135
3.944LysLys: 3.944 ± 0.108
6.313LysLeu: 6.313 ± 0.133
1.405LysMet: 1.405 ± 0.062
2.225LysAsn: 2.225 ± 0.083
4.068LysPro: 4.068 ± 0.098
1.452LysGln: 1.452 ± 0.068
3.094LysArg: 3.094 ± 0.093
2.917LysSer: 2.917 ± 0.096
4.168LysThr: 4.168 ± 0.114
4.397LysVal: 4.397 ± 0.11
0.75LysTrp: 0.75 ± 0.047
3.222LysTyr: 3.222 ± 0.098
0.0LysXaa: 0.0 ± 0.0
Leu
8.598LeuAla: 8.598 ± 0.185
0.564LeuCys: 0.564 ± 0.036
4.682LeuAsp: 4.682 ± 0.107
8.412LeuGlu: 8.412 ± 0.195
3.836LeuPhe: 3.836 ± 0.11
7.649LeuGly: 7.649 ± 0.157
1.669LeuHis: 1.669 ± 0.065
8.11LeuIle: 8.11 ± 0.168
7.992LeuLys: 7.992 ± 0.134
11.453LeuLeu: 11.453 ± 0.219
2.421LeuMet: 2.421 ± 0.085
4.392LeuAsn: 4.392 ± 0.106
4.196LeuPro: 4.196 ± 0.107
2.016LeuGln: 2.016 ± 0.067
6.348LeuArg: 6.348 ± 0.133
7.919LeuSer: 7.919 ± 0.133
6.021LeuThr: 6.021 ± 0.146
8.913LeuVal: 8.913 ± 0.155
0.979LeuTrp: 0.979 ± 0.044
3.954LeuTyr: 3.954 ± 0.103
0.0LeuXaa: 0.0 ± 0.0
Met
1.377MetAla: 1.377 ± 0.061
0.103MetCys: 0.103 ± 0.017
1.082MetAsp: 1.082 ± 0.057
1.47MetGlu: 1.47 ± 0.065
0.836MetPhe: 0.836 ± 0.05
1.674MetGly: 1.674 ± 0.068
0.332MetHis: 0.332 ± 0.029
1.707MetIle: 1.707 ± 0.071
1.49MetLys: 1.49 ± 0.063
2.409MetLeu: 2.409 ± 0.083
0.544MetMet: 0.544 ± 0.041
0.732MetAsn: 0.732 ± 0.044
0.972MetPro: 0.972 ± 0.052
0.31MetGln: 0.31 ± 0.026
1.274MetArg: 1.274 ± 0.052
1.591MetSer: 1.591 ± 0.065
0.821MetThr: 0.821 ± 0.05
2.077MetVal: 2.077 ± 0.073
0.209MetTrp: 0.209 ± 0.023
0.745MetTyr: 0.745 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
2.306AsnAla: 2.306 ± 0.095
0.292AsnCys: 0.292 ± 0.029
1.186AsnAsp: 1.186 ± 0.052
1.855AsnGlu: 1.855 ± 0.076
0.866AsnPhe: 0.866 ± 0.045
2.459AsnGly: 2.459 ± 0.084
0.586AsnHis: 0.586 ± 0.036
3.285AsnIle: 3.285 ± 0.087
1.752AsnLys: 1.752 ± 0.066
3.365AsnLeu: 3.365 ± 0.099
0.717AsnMet: 0.717 ± 0.04
1.218AsnAsn: 1.218 ± 0.053
2.799AsnPro: 2.799 ± 0.089
0.687AsnGln: 0.687 ± 0.042
1.485AsnArg: 1.485 ± 0.063
1.724AsnSer: 1.724 ± 0.073
2.124AsnThr: 2.124 ± 0.084
2.887AsnVal: 2.887 ± 0.087
0.378AsnTrp: 0.378 ± 0.032
1.591AsnTyr: 1.591 ± 0.061
0.0AsnXaa: 0.0 ± 0.0
Pro
2.615ProAla: 2.615 ± 0.077
0.332ProCys: 0.332 ± 0.026
2.177ProAsp: 2.177 ± 0.079
3.67ProGlu: 3.67 ± 0.087
1.948ProPhe: 1.948 ± 0.071
4.322ProGly: 4.322 ± 0.111
0.823ProHis: 0.823 ± 0.046
2.537ProIle: 2.537 ± 0.085
1.676ProLys: 1.676 ± 0.075
5.044ProLeu: 5.044 ± 0.114
0.765ProMet: 0.765 ± 0.041
1.009ProAsn: 1.009 ± 0.046
2.605ProPro: 2.605 ± 0.088
0.914ProGln: 0.914 ± 0.047
2.676ProArg: 2.676 ± 0.08
3.388ProSer: 3.388 ± 0.082
1.926ProThr: 1.926 ± 0.064
4.757ProVal: 4.757 ± 0.1
0.665ProTrp: 0.665 ± 0.044
1.888ProTyr: 1.888 ± 0.065
0.0ProXaa: 0.0 ± 0.0
Gln
1.618GlnAla: 1.618 ± 0.063
0.141GlnCys: 0.141 ± 0.019
0.732GlnAsp: 0.732 ± 0.038
1.269GlnGlu: 1.269 ± 0.055
0.446GlnPhe: 0.446 ± 0.03
1.598GlnGly: 1.598 ± 0.062
0.34GlnHis: 0.34 ± 0.03
1.08GlnIle: 1.08 ± 0.056
0.936GlnLys: 0.936 ± 0.055
2.152GlnLeu: 2.152 ± 0.079
0.335GlnMet: 0.335 ± 0.027
0.539GlnAsn: 0.539 ± 0.038
1.052GlnPro: 1.052 ± 0.055
0.597GlnGln: 0.597 ± 0.039
1.06GlnArg: 1.06 ± 0.052
1.002GlnSer: 1.002 ± 0.051
0.826GlnThr: 0.826 ± 0.05
1.916GlnVal: 1.916 ± 0.07
0.257GlnTrp: 0.257 ± 0.024
0.853GlnTyr: 0.853 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
3.355ArgAla: 3.355 ± 0.081
0.415ArgCys: 0.415 ± 0.034
2.759ArgAsp: 2.759 ± 0.084
5.19ArgGlu: 5.19 ± 0.135
2.313ArgPhe: 2.313 ± 0.079
3.881ArgGly: 3.881 ± 0.112
0.936ArgHis: 0.936 ± 0.048
4.843ArgIle: 4.843 ± 0.111
3.992ArgLys: 3.992 ± 0.104
6.343ArgLeu: 6.343 ± 0.132
1.289ArgMet: 1.289 ± 0.055
2.014ArgAsn: 2.014 ± 0.065
1.837ArgPro: 1.837 ± 0.069
0.984ArgGln: 0.984 ± 0.053
4.027ArgArg: 4.027 ± 0.128
2.807ArgSer: 2.807 ± 0.087
2.051ArgThr: 2.051 ± 0.063
5.336ArgVal: 5.336 ± 0.133
0.461ArgTrp: 0.461 ± 0.037
1.883ArgTyr: 1.883 ± 0.07
0.0ArgXaa: 0.0 ± 0.0
Ser
3.31SerAla: 3.31 ± 0.103
0.415SerCys: 0.415 ± 0.034
2.464SerAsp: 2.464 ± 0.081
3.944SerGlu: 3.944 ± 0.088
2.721SerPhe: 2.721 ± 0.094
4.365SerGly: 4.365 ± 0.106
1.007SerHis: 1.007 ± 0.056
5.767SerIle: 5.767 ± 0.126
4.095SerLys: 4.095 ± 0.112
7.491SerLeu: 7.491 ± 0.174
1.656SerMet: 1.656 ± 0.068
2.22SerAsn: 2.22 ± 0.071
2.854SerPro: 2.854 ± 0.083
1.445SerGln: 1.445 ± 0.06
3.995SerArg: 3.995 ± 0.101
4.777SerSer: 4.777 ± 0.132
3.481SerThr: 3.481 ± 0.098
4.712SerVal: 4.712 ± 0.123
0.715SerTrp: 0.715 ± 0.044
2.336SerTyr: 2.336 ± 0.084
0.0SerXaa: 0.0 ± 0.0
Thr
3.295ThrAla: 3.295 ± 0.097
0.415ThrCys: 0.415 ± 0.033
1.669ThrAsp: 1.669 ± 0.066
2.051ThrGlu: 2.051 ± 0.071
1.52ThrPhe: 1.52 ± 0.062
5.09ThrGly: 5.09 ± 0.136
0.826ThrHis: 0.826 ± 0.044
4.221ThrIle: 4.221 ± 0.12
1.631ThrLys: 1.631 ± 0.063
5.699ThrLeu: 5.699 ± 0.127
1.085ThrMet: 1.085 ± 0.051
1.123ThrAsn: 1.123 ± 0.048
3.287ThrPro: 3.287 ± 0.096
0.939ThrGln: 0.939 ± 0.05
2.927ThrArg: 2.927 ± 0.088
3.35ThrSer: 3.35 ± 0.102
2.837ThrThr: 2.837 ± 0.166
4.508ThrVal: 4.508 ± 0.131
0.634ThrTrp: 0.634 ± 0.044
2.009ThrTyr: 2.009 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
6.109ValAla: 6.109 ± 0.12
0.619ValCys: 0.619 ± 0.043
4.556ValAsp: 4.556 ± 0.105
8.11ValGlu: 8.11 ± 0.172
4.508ValPhe: 4.508 ± 0.117
5.067ValGly: 5.067 ± 0.122
1.196ValHis: 1.196 ± 0.053
5.608ValIle: 5.608 ± 0.12
7.315ValLys: 7.315 ± 0.158
9.248ValLeu: 9.248 ± 0.176
1.689ValMet: 1.689 ± 0.059
3.423ValAsn: 3.423 ± 0.098
2.698ValPro: 2.698 ± 0.086
1.402ValGln: 1.402 ± 0.064
4.81ValArg: 4.81 ± 0.114
6.24ValSer: 6.24 ± 0.112
3.688ValThr: 3.688 ± 0.106
8.103ValVal: 8.103 ± 0.177
0.712ValTrp: 0.712 ± 0.039
3.861ValTyr: 3.861 ± 0.108
0.0ValXaa: 0.0 ± 0.0
Trp
0.571TrpAla: 0.571 ± 0.039
0.081TrpCys: 0.081 ± 0.013
0.493TrpAsp: 0.493 ± 0.034
0.74TrpGlu: 0.74 ± 0.042
0.581TrpPhe: 0.581 ± 0.037
0.79TrpGly: 0.79 ± 0.045
0.169TrpHis: 0.169 ± 0.022
0.878TrpIle: 0.878 ± 0.049
0.629TrpLys: 0.629 ± 0.041
1.309TrpLeu: 1.309 ± 0.061
0.335TrpMet: 0.335 ± 0.029
0.383TrpAsn: 0.383 ± 0.034
0.352TrpPro: 0.352 ± 0.033
0.171TrpGln: 0.171 ± 0.02
0.856TrpArg: 0.856 ± 0.049
0.778TrpSer: 0.778 ± 0.048
0.41TrpThr: 0.41 ± 0.036
1.012TrpVal: 1.012 ± 0.051
0.229TrpTrp: 0.229 ± 0.024
0.43TrpTyr: 0.43 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.759TyrAla: 2.759 ± 0.07
0.39TyrCys: 0.39 ± 0.03
1.885TyrAsp: 1.885 ± 0.069
2.562TyrGlu: 2.562 ± 0.072
1.636TyrPhe: 1.636 ± 0.07
3.509TyrGly: 3.509 ± 0.098
0.637TyrHis: 0.637 ± 0.042
2.605TyrIle: 2.605 ± 0.076
2.024TyrLys: 2.024 ± 0.068
4.624TyrLeu: 4.624 ± 0.105
1.009TyrMet: 1.009 ± 0.05
1.51TyrAsn: 1.51 ± 0.058
1.883TyrPro: 1.883 ± 0.071
0.848TyrGln: 0.848 ± 0.049
2.552TyrArg: 2.552 ± 0.08
3.003TyrSer: 3.003 ± 0.1
2.064TyrThr: 2.064 ± 0.075
3.929TyrVal: 3.929 ± 0.09
0.569TyrTrp: 0.569 ± 0.041
2.087TyrTyr: 2.087 ± 0.074
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1387 proteins (397285 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski