Sphingobacterium sp. IITKGP-BTPF85

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3C3V2|A0A0M3C3V2_9SPHI Uncharacterized protein OS=Sphingobacterium sp. IITKGP-BTPF85 OX=1338009 GN=L950_0226845 PE=4 SV=1
MM1 pKa = 7.54SLTEE5 pKa = 3.9YY6 pKa = 10.46LDD8 pKa = 4.06YY9 pKa = 11.29VILPILILSTVITFIRR25 pKa = 11.84LFKK28 pKa = 11.02GPDD31 pKa = 2.97VADD34 pKa = 3.63RR35 pKa = 11.84VIALDD40 pKa = 4.53LIITTGIGIITVYY53 pKa = 10.69SITTSQEE60 pKa = 3.1IFLDD64 pKa = 3.48VAMILALIAFLGTIAFAYY82 pKa = 10.24YY83 pKa = 10.45LEE85 pKa = 4.42KK86 pKa = 10.25QDD88 pKa = 4.79YY89 pKa = 10.61NDD91 pKa = 3.28

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3CEP9|A0A0M3CEP9_9SPHI Uncharacterized protein OS=Sphingobacterium sp. IITKGP-BTPF85 OX=1338009 GN=L950_0212965 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.59LNDD5 pKa = 3.48IRR7 pKa = 11.84FGALGGTICSIWASFSFGDD26 pKa = 3.93ILQTVCMAAVGTIVSYY42 pKa = 9.11ITSRR46 pKa = 11.84LVSRR50 pKa = 11.84IRR52 pKa = 11.84KK53 pKa = 8.76RR54 pKa = 3.21

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6019

0

6019

1462207

29

1743

242.9

27.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.698 ± 0.033

0.757 ± 0.01

5.416 ± 0.024

5.946 ± 0.031

4.991 ± 0.027

6.374 ± 0.032

1.926 ± 0.016

7.863 ± 0.04

7.348 ± 0.031

9.683 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.017

5.828 ± 0.035

3.474 ± 0.019

3.905 ± 0.021

3.802 ± 0.021

6.56 ± 0.026

5.482 ± 0.023

6.078 ± 0.029

1.195 ± 0.013

4.255 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski