Fructobacillus durionis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Fructobacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1GX56|A0A1I1GX56_9LACO PTS_EIIC domain-containing protein OS=Fructobacillus durionis OX=283737 GN=SAMN05660453_1140 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 4.86NQTMKK7 pKa = 10.69QLSKK11 pKa = 11.11RR12 pKa = 11.84LMVTAGASTLAATGAVLGATVVGNAQTASANDD44 pKa = 3.4VTEE47 pKa = 4.39PTSQTDD53 pKa = 4.0AQNQWKK59 pKa = 9.94ANSVDD64 pKa = 3.52HH65 pKa = 6.59VKK67 pKa = 10.36QAVKK71 pKa = 10.24KK72 pKa = 10.53QSAKK76 pKa = 10.42DD77 pKa = 3.51LKK79 pKa = 10.69DD80 pKa = 3.82YY81 pKa = 10.37QVQWGDD87 pKa = 3.52TLSAIAEE94 pKa = 4.35AFGTTADD101 pKa = 4.23AAGQQLGLSDD111 pKa = 4.5HH112 pKa = 6.6GLLVAGQTMGKK123 pKa = 8.51QEE125 pKa = 4.88EE126 pKa = 4.6IIQGLKK132 pKa = 7.65TAGYY136 pKa = 9.43LADD139 pKa = 4.18GQQTQQADD147 pKa = 3.79LGKK150 pKa = 10.54GDD152 pKa = 3.71QNLIMVQQGTPTLDD166 pKa = 3.45TNNGQQTTEE175 pKa = 3.7NTTVNNQTNSQSGSQAAYY193 pKa = 9.3EE194 pKa = 4.2QASQAAAEE202 pKa = 4.28SQAISQSNSMAAATAATNQYY222 pKa = 8.85SQSAASQANNDD233 pKa = 3.46ASQASLTADD242 pKa = 3.4SEE244 pKa = 4.96SQVTPAASDD253 pKa = 3.42ATSTSDD259 pKa = 4.21DD260 pKa = 3.67AAATTPASDD269 pKa = 3.55SSASNSDD276 pKa = 3.14SDD278 pKa = 4.32NTDD281 pKa = 3.79DD282 pKa = 6.02SNDD285 pKa = 3.79DD286 pKa = 3.47QPSARR291 pKa = 11.84MADD294 pKa = 4.07DD295 pKa = 5.11DD296 pKa = 5.87SSDD299 pKa = 3.72NNQGSVTPTAYY310 pKa = 9.49TPATNSDD317 pKa = 3.62GGSQVTPASTPAATNRR333 pKa = 11.84VNTEE337 pKa = 3.51ALINWFYY344 pKa = 11.81NHH346 pKa = 6.4MGKK349 pKa = 8.26LTYY352 pKa = 11.2DD353 pKa = 3.12MGGSRR358 pKa = 11.84NGSDD362 pKa = 3.01GTADD366 pKa = 3.58CSGSMTEE373 pKa = 4.5ALYY376 pKa = 10.53EE377 pKa = 4.27AGASKK382 pKa = 9.89PAYY385 pKa = 10.14LYY387 pKa = 9.76STEE390 pKa = 4.31NLHH393 pKa = 7.53SYY395 pKa = 7.97LTQNGYY401 pKa = 9.86HH402 pKa = 6.43LVAVDD407 pKa = 4.31QPWDD411 pKa = 3.52AQRR414 pKa = 11.84GDD416 pKa = 3.66VVIWGQKK423 pKa = 8.47GASLGSAGHH432 pKa = 5.83AQVMVDD438 pKa = 3.34QNNAISVNYY447 pKa = 10.25AHH449 pKa = 7.62GEE451 pKa = 4.0QQGAAVSVWNYY462 pKa = 10.38ANAYY466 pKa = 10.07AYY468 pKa = 10.18NQEE471 pKa = 4.23ANGGHH476 pKa = 5.83LTYY479 pKa = 10.62YY480 pKa = 10.52VYY482 pKa = 10.63RR483 pKa = 11.84QQ484 pKa = 3.09

Molecular weight: 50.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1HNL8|A0A1I1HNL8_9LACO Helix-turn-helix domain-containing protein OS=Fructobacillus durionis OX=283737 GN=SAMN05660453_0018 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.02KK9 pKa = 7.4RR10 pKa = 11.84HH11 pKa = 5.36RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.84GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MLTSNGRR28 pKa = 11.84KK29 pKa = 8.63VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1250

0

1250

394407

34

2816

315.5

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.274 ± 0.092

0.248 ± 0.013

6.155 ± 0.069

6.02 ± 0.08

4.264 ± 0.056

6.917 ± 0.063

1.825 ± 0.027

6.269 ± 0.066

6.726 ± 0.062

9.781 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.032

4.436 ± 0.06

3.507 ± 0.042

4.517 ± 0.061

3.856 ± 0.056

6.046 ± 0.076

5.756 ± 0.067

7.419 ± 0.056

1.048 ± 0.027

3.301 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski