Hyphantria cunea nuclear polyhedrosis virus (HcNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2NP17|Q2NP17_NPVHC ORF94 peptide OS=Hyphantria cunea nuclear polyhedrosis virus OX=28288 GN=HynVgp094 PE=4 SV=1
MM1 pKa = 7.33AHH3 pKa = 7.11ALSAADD9 pKa = 3.77VDD11 pKa = 6.17LIACVLNDD19 pKa = 3.33NLFLVGNSYY28 pKa = 10.76VMCNVFDD35 pKa = 3.85QEE37 pKa = 4.23AGQVEE42 pKa = 5.02SVCLGEE48 pKa = 4.86IGAAQAHH55 pKa = 5.5TTDD58 pKa = 3.11QDD60 pKa = 3.65AVLDD64 pKa = 4.16ASSTSDD70 pKa = 3.51VPSSTSSQRR79 pKa = 11.84VPP81 pKa = 3.26

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2NNU9|Q2NNU9_NPVHC ORF40 peptide OS=Hyphantria cunea nuclear polyhedrosis virus OX=28288 GN=HynVgp040 PE=4 SV=1
MM1 pKa = 7.54SSNAIFTFLTEE12 pKa = 4.19YY13 pKa = 10.42VASSSNSVLTEE24 pKa = 3.7ALTCARR30 pKa = 11.84SKK32 pKa = 11.09YY33 pKa = 9.71CAANALAFSALNGSYY48 pKa = 9.62TKK50 pKa = 10.78SLLPTNEE57 pKa = 3.7RR58 pKa = 11.84SRR60 pKa = 11.84LNSPSCKK67 pKa = 9.77KK68 pKa = 10.14RR69 pKa = 11.84GHH71 pKa = 5.01VDD73 pKa = 2.6ARR75 pKa = 11.84KK76 pKa = 9.66RR77 pKa = 11.84EE78 pKa = 3.89RR79 pKa = 11.84LAKK82 pKa = 9.11IVRR85 pKa = 11.84VFVMRR90 pKa = 4.64

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

148

0

148

39694

50

1230

268.2

30.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.394 ± 0.228

2.544 ± 0.149

5.865 ± 0.128

5.031 ± 0.144

4.789 ± 0.12

3.781 ± 0.165

2.464 ± 0.093

5.071 ± 0.118

5.842 ± 0.208

9.687 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.075

6.88 ± 0.193

4.21 ± 0.193

4.288 ± 0.199

5.696 ± 0.183

5.618 ± 0.155

5.938 ± 0.151

7.46 ± 0.16

0.864 ± 0.069

4.038 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski