Dragonfly larvae associated circular virus-2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5U7Q2|W5U7Q2_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-2 OX=1454023 PE=4 SV=1
MM1 pKa = 7.71ARR3 pKa = 11.84RR4 pKa = 11.84QGIFWLLTIPRR15 pKa = 11.84HH16 pKa = 5.03EE17 pKa = 4.45FVPYY21 pKa = 10.54LPPGCVWIRR30 pKa = 11.84GQLEE34 pKa = 3.87EE35 pKa = 4.79GGEE38 pKa = 4.06DD39 pKa = 5.65GYY41 pKa = 10.82LHH43 pKa = 6.0WQVMVAFTKK52 pKa = 10.54KK53 pKa = 9.89IGLSGVKK60 pKa = 10.2VLFGPAAHH68 pKa = 7.05AEE70 pKa = 4.19LSRR73 pKa = 11.84SAAATQYY80 pKa = 9.33VWKK83 pKa = 10.38EE84 pKa = 3.9EE85 pKa = 3.94TRR87 pKa = 11.84VAGTQFNLGAKK98 pKa = 9.51PFEE101 pKa = 4.68RR102 pKa = 11.84NSKK105 pKa = 10.23RR106 pKa = 11.84DD107 pKa = 2.95WDD109 pKa = 4.66SIWLSAQEE117 pKa = 4.51GNLEE121 pKa = 4.87AIPADD126 pKa = 3.44VRR128 pKa = 11.84VVSYY132 pKa = 8.54RR133 pKa = 11.84TLRR136 pKa = 11.84AIGSDD141 pKa = 3.69YY142 pKa = 11.17AVPRR146 pKa = 11.84ALEE149 pKa = 4.0RR150 pKa = 11.84TCYY153 pKa = 10.23VFWGSTGTGKK163 pKa = 10.38SRR165 pKa = 11.84TAWEE169 pKa = 4.0QAGMDD174 pKa = 4.85AYY176 pKa = 11.26CKK178 pKa = 10.38DD179 pKa = 3.65PRR181 pKa = 11.84SKK183 pKa = 10.54FWDD186 pKa = 5.21GYY188 pKa = 9.86CSQEE192 pKa = 3.68NVVIDD197 pKa = 3.95EE198 pKa = 4.19FRR200 pKa = 11.84GGIDD204 pKa = 2.84ISHH207 pKa = 7.48ILRR210 pKa = 11.84WLDD213 pKa = 3.42RR214 pKa = 11.84YY215 pKa = 9.28PVRR218 pKa = 11.84VEE220 pKa = 3.72IKK222 pKa = 10.01GSSRR226 pKa = 11.84PLQATKK232 pKa = 10.67LWITSNLDD240 pKa = 3.26PNLWYY245 pKa = 9.87PDD247 pKa = 3.04IDD249 pKa = 4.69AEE251 pKa = 4.35TLLALRR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84LTITHH264 pKa = 6.96FAA266 pKa = 4.02

Molecular weight: 30.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5U7Q2|W5U7Q2_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-2 OX=1454023 PE=4 SV=1
MM1 pKa = 7.38TKK3 pKa = 10.47LHH5 pKa = 6.05VPYY8 pKa = 7.6MTPYY12 pKa = 10.88RR13 pKa = 11.84NGTSQSHH20 pKa = 5.7GKK22 pKa = 9.76RR23 pKa = 11.84FKK25 pKa = 11.33LEE27 pKa = 3.87VKK29 pKa = 10.45GEE31 pKa = 4.02YY32 pKa = 10.04KK33 pKa = 9.43RR34 pKa = 11.84THH36 pKa = 5.7SQTTTEE42 pKa = 3.98KK43 pKa = 10.97AEE45 pKa = 4.0EE46 pKa = 4.09RR47 pKa = 11.84MHH49 pKa = 7.55GDD51 pKa = 3.7DD52 pKa = 3.81VQSGITGTGMSFPGHH67 pKa = 5.92SRR69 pKa = 11.84HH70 pKa = 6.73KK71 pKa = 10.79DD72 pKa = 3.24VLRR75 pKa = 11.84YY76 pKa = 9.63AQVKK80 pKa = 9.74NNEE83 pKa = 4.25TYY85 pKa = 11.22GLIVDD90 pKa = 4.93CVAGSQATVTLATVGNTEE108 pKa = 3.79QCIGKK113 pKa = 9.43VNPPGPFTTSLPVWKK128 pKa = 9.82AQIDD132 pKa = 3.73AATVLNSPWTASDD145 pKa = 3.48GSNNAMQKK153 pKa = 10.28INIGFCHH160 pKa = 5.85HH161 pKa = 7.2TIDD164 pKa = 4.25FTNVTNAAAVVDD176 pKa = 4.54VYY178 pKa = 11.43VVEE181 pKa = 4.66CKK183 pKa = 10.03QDD185 pKa = 3.04IPAINAGRR193 pKa = 11.84HH194 pKa = 4.58GFLGIWAHH202 pKa = 6.86GEE204 pKa = 4.08GTEE207 pKa = 4.32SGGLASQTVTTTTATLGSSSFNVVGAKK234 pKa = 8.3PTDD237 pKa = 2.9ISLFKK242 pKa = 10.54KK243 pKa = 10.1YY244 pKa = 11.04YY245 pKa = 9.82KK246 pKa = 10.12VLKK249 pKa = 8.58AHH251 pKa = 6.52CMHH254 pKa = 7.42LGPGATEE261 pKa = 3.95VVNISSACNVDD272 pKa = 3.4VDD274 pKa = 4.03IEE276 pKa = 4.33KK277 pKa = 11.04YY278 pKa = 9.4LAKK281 pKa = 10.9NNVLVPDD288 pKa = 4.56LADD291 pKa = 2.97TAMKK295 pKa = 9.36IWAMKK300 pKa = 10.35GSFEE304 pKa = 4.38IYY306 pKa = 10.18CIARR310 pKa = 11.84GTAMLDD316 pKa = 3.54TNVLDD321 pKa = 4.8LANTTTAPVKK331 pKa = 10.33LAVVVQRR338 pKa = 11.84KK339 pKa = 7.42YY340 pKa = 10.55QFNPMKK346 pKa = 10.5YY347 pKa = 9.22KK348 pKa = 10.56SKK350 pKa = 10.58SFKK353 pKa = 10.93AVVGYY358 pKa = 10.21QKK360 pKa = 11.06LPFGVPAANLKK371 pKa = 9.24TEE373 pKa = 4.36GPTANISVAFTAAAA387 pKa = 3.71

Molecular weight: 41.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

653

266

387

326.5

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.495 ± 0.547

1.685 ± 0.117

4.594 ± 0.432

4.594 ± 0.917

3.522 ± 0.153

7.963 ± 0.044

2.603 ± 0.467

4.9 ± 0.476

6.126 ± 1.284

6.891 ± 1.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.557

4.288 ± 1.311

4.594 ± 0.189

3.369 ± 0.009

4.9 ± 2.417

5.972 ± 0.027

8.27 ± 1.697

8.27 ± 1.212

2.45 ± 1.33

3.522 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski