Microviridae Fen418_41

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UME7|A0A0G2UME7_9VIRU Uncharacterized protein OS=Microviridae Fen418_41 OX=1655653 PE=4 SV=1
MM1 pKa = 7.21WRR3 pKa = 11.84HH4 pKa = 5.51GVAIMIIVYY13 pKa = 10.09DD14 pKa = 3.76HH15 pKa = 7.31LIFLSKK21 pKa = 9.88RR22 pKa = 11.84TMVFLFIFLQTTAAGGGISVLGALAGAIPMVGGIISGLINNSNQDD67 pKa = 3.04QVNASNQAFQLAQQQQIEE85 pKa = 4.76AYY87 pKa = 8.45NTAQWNAQNAYY98 pKa = 9.79NSPEE102 pKa = 3.91EE103 pKa = 4.0VMQRR107 pKa = 11.84YY108 pKa = 8.82EE109 pKa = 4.66DD110 pKa = 4.1AGLNPNLIYY119 pKa = 10.76GSGSGTGNLAEE130 pKa = 4.63MPQPAPRR137 pKa = 11.84VEE139 pKa = 5.21FVAQAHH145 pKa = 6.51PNFDD149 pKa = 3.97LGTAAAQSVQAYY161 pKa = 9.02NNTVSTVNQGNLVKK175 pKa = 10.51QQSINVAQNTALQAAQEE192 pKa = 4.17ININADD198 pKa = 2.98TGLKK202 pKa = 10.38DD203 pKa = 3.62NQEE206 pKa = 4.03AAQLISNLYY215 pKa = 10.51SGDD218 pKa = 3.59SMKK221 pKa = 11.3ANIALMKK228 pKa = 10.56AQTQLVLTNQQTAQLMQQPNLVTALQNITNMQEE261 pKa = 3.94NNQLTQAQIANINANIAQTQIKK283 pKa = 8.59TKK285 pKa = 10.54LDD287 pKa = 3.41QLNLYY292 pKa = 8.48LQNHH296 pKa = 6.46GLNLDD301 pKa = 3.64SSLMSRR307 pKa = 11.84VGSIVLQNLSKK318 pKa = 10.24STVASKK324 pKa = 10.46MNSADD329 pKa = 3.7NSPDD333 pKa = 3.45MNDD336 pKa = 2.81FDD338 pKa = 5.08YY339 pKa = 11.54NYY341 pKa = 11.38

Molecular weight: 36.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK30|A0A0G2UK30_9VIRU DNA pilot protein VP2 OS=Microviridae Fen418_41 OX=1655653 PE=4 SV=1
MM1 pKa = 7.47IVFLGLISCGSISFLTLFLLITPVIVLTLLIRR33 pKa = 11.84SFLSLGVIMTVGLTSLFALSGRR55 pKa = 11.84KK56 pKa = 9.02KK57 pKa = 10.3KK58 pKa = 11.01YY59 pKa = 9.01EE60 pKa = 3.74LSFSIMAKK68 pKa = 8.39KK69 pKa = 8.7TLINMQQITNIYY81 pKa = 7.75MPCRR85 pKa = 11.84FPFQTKK91 pKa = 9.97DD92 pKa = 3.28GAIVPCGKK100 pKa = 9.97CDD102 pKa = 3.32SCLTRR107 pKa = 11.84MANGWAFRR115 pKa = 11.84VMQEE119 pKa = 3.89EE120 pKa = 4.46KK121 pKa = 10.38KK122 pKa = 10.35AKK124 pKa = 9.08CSMWITLTYY133 pKa = 9.85DD134 pKa = 3.25TDD136 pKa = 4.3HH137 pKa = 7.2VPLTNNGYY145 pKa = 7.1MTLNRR150 pKa = 11.84VHH152 pKa = 6.05TQLFFKK158 pKa = 10.67RR159 pKa = 11.84LRR161 pKa = 11.84RR162 pKa = 11.84FQNIHH167 pKa = 6.68NINQEE172 pKa = 4.2RR173 pKa = 11.84IYY175 pKa = 10.63DD176 pKa = 3.52TGDD179 pKa = 2.84RR180 pKa = 11.84FGRR183 pKa = 11.84LQYY186 pKa = 9.27PIKK189 pKa = 10.76YY190 pKa = 9.16FGCGEE195 pKa = 4.19YY196 pKa = 8.89GTKK199 pKa = 9.82YY200 pKa = 10.09RR201 pKa = 11.84RR202 pKa = 11.84PHH204 pKa = 4.56YY205 pKa = 9.79HH206 pKa = 6.56ICLLNCDD213 pKa = 5.03EE214 pKa = 4.22RR215 pKa = 11.84ALWYY219 pKa = 10.03SWQDD223 pKa = 3.22GEE225 pKa = 4.53TGLPFGDD232 pKa = 4.25VYY234 pKa = 11.04IDD236 pKa = 4.6DD237 pKa = 4.9RR238 pKa = 11.84PLSAAAIAYY247 pKa = 7.11TCFYY251 pKa = 9.01MNKK254 pKa = 8.23PKK256 pKa = 10.36RR257 pKa = 11.84VPEE260 pKa = 4.09HH261 pKa = 6.78KK262 pKa = 10.52NDD264 pKa = 3.23DD265 pKa = 3.68RR266 pKa = 11.84EE267 pKa = 4.21RR268 pKa = 11.84EE269 pKa = 3.87FRR271 pKa = 11.84MMSTKK276 pKa = 9.91MGLGYY281 pKa = 8.37LTPDD285 pKa = 2.91TLAYY289 pKa = 10.16HH290 pKa = 6.69KK291 pKa = 10.94ADD293 pKa = 3.61LSRR296 pKa = 11.84SYY298 pKa = 9.96FTTEE302 pKa = 3.8GGGKK306 pKa = 9.72AALPRR311 pKa = 11.84YY312 pKa = 9.12LADD315 pKa = 5.24RR316 pKa = 11.84IFTPEE321 pKa = 4.1EE322 pKa = 3.92KK323 pKa = 10.43ASQQPLINEE332 pKa = 4.14RR333 pKa = 11.84NSLQFNLEE341 pKa = 3.55FDD343 pKa = 3.35EE344 pKa = 4.64HH345 pKa = 6.19KK346 pKa = 10.69RR347 pKa = 11.84RR348 pKa = 11.84TGGNRR353 pKa = 11.84QTFQQEE359 pKa = 4.02TNDD362 pKa = 3.33RR363 pKa = 11.84RR364 pKa = 11.84RR365 pKa = 11.84EE366 pKa = 4.2SIRR369 pKa = 11.84SFQRR373 pKa = 11.84KK374 pKa = 8.43ARR376 pKa = 11.84PDD378 pKa = 3.21GMM380 pKa = 4.86

Molecular weight: 44.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1508

115

551

301.6

33.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.891 ± 1.228

1.26 ± 0.481

4.841 ± 0.958

4.31 ± 0.673

4.31 ± 0.698

6.83 ± 1.115

1.923 ± 0.238

6.366 ± 0.168

3.581 ± 0.908

8.554 ± 0.74

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.249 ± 0.334

6.83 ± 1.483

4.973 ± 0.954

6.3 ± 1.528

5.637 ± 1.496

6.101 ± 0.356

7.029 ± 0.812

5.106 ± 0.762

1.393 ± 0.451

3.515 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski