Pseudomonas phage AAT-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pamexvirus; unclassified Pamexvirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125SA91|A0A125SA91_9CAUD Putative DNA binding protein OS=Pseudomonas phage AAT-1 OX=1775248 GN=AAT1_02061 PE=4 SV=1
MM1 pKa = 7.1AQEE4 pKa = 4.31LLAMVAEE11 pKa = 4.8VAEE14 pKa = 4.26EE15 pKa = 3.82MDD17 pKa = 4.33GVEE20 pKa = 5.3LYY22 pKa = 10.04PNYY25 pKa = 10.08SGRR28 pKa = 11.84VMFGRR33 pKa = 11.84TCAGFTLDD41 pKa = 5.24RR42 pKa = 11.84DD43 pKa = 4.0VTPLAFFGQLLEE55 pKa = 4.71AANDD59 pKa = 3.22MDD61 pKa = 5.97AEE63 pKa = 4.21DD64 pKa = 4.51RR65 pKa = 11.84EE66 pKa = 4.96AILSVLPEE74 pKa = 4.04MMQDD78 pKa = 3.16ARR80 pKa = 11.84TDD82 pKa = 3.31AMGLGTILYY91 pKa = 8.74FPSFYY96 pKa = 11.2VPGFEE101 pKa = 4.72GG102 pKa = 3.52

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125SA88|A0A125SA88_9CAUD Uncharacterized protein OS=Pseudomonas phage AAT-1 OX=1775248 GN=AAT1_02058 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52AFLRR6 pKa = 11.84WVIVLLLTGGVRR18 pKa = 11.84SPLRR22 pKa = 11.84HH23 pKa = 5.42MEE25 pKa = 3.89EE26 pKa = 3.74GRR28 pKa = 11.84PYY30 pKa = 9.96YY31 pKa = 10.02VVRR34 pKa = 11.84HH35 pKa = 5.15GRR37 pKa = 11.84RR38 pKa = 11.84LSIEE42 pKa = 4.2RR43 pKa = 11.84VDD45 pKa = 3.79STLKK49 pKa = 10.6LPAVRR54 pKa = 11.84RR55 pKa = 11.84FKK57 pKa = 11.03RR58 pKa = 11.84GDD60 pKa = 2.99RR61 pKa = 11.84VAATRR66 pKa = 11.84SAGFHH71 pKa = 6.61PGAHH75 pKa = 4.92GVVVFQEE82 pKa = 4.22PNPVGKK88 pKa = 10.39VWVLRR93 pKa = 11.84DD94 pKa = 3.57GASSEE99 pKa = 4.18VFYY102 pKa = 11.07YY103 pKa = 10.04PHH105 pKa = 6.85EE106 pKa = 5.02LEE108 pKa = 5.83DD109 pKa = 3.7EE110 pKa = 4.3

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

17756

61

1202

233.6

25.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.224 ± 0.485

1.104 ± 0.139

6.325 ± 0.277

6.877 ± 0.341

3.205 ± 0.174

8.318 ± 0.261

1.926 ± 0.149

3.137 ± 0.187

4.68 ± 0.385

8.628 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.134

2.777 ± 0.166

5.148 ± 0.261

3.435 ± 0.203

7.147 ± 0.303

5.694 ± 0.241

5.57 ± 0.269

7.603 ± 0.251

1.926 ± 0.171

2.771 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski