Pseudomonas phage Psp6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PI54|A0A2H4PI54_9CAUD Uncharacterized protein OS=Pseudomonas phage Psp6 OX=2053693 GN=Psp6_00053 PE=4 SV=1
MM1 pKa = 7.62SYY3 pKa = 8.82VTLAQAKK10 pKa = 8.74AQLLVIHH17 pKa = 7.08DD18 pKa = 4.87ADD20 pKa = 4.57DD21 pKa = 3.41EE22 pKa = 4.91HH23 pKa = 7.77IQLLIDD29 pKa = 3.74AAEE32 pKa = 4.56SYY34 pKa = 10.01CASYY38 pKa = 10.28MNRR41 pKa = 11.84PAITDD46 pKa = 3.5YY47 pKa = 11.1QEE49 pKa = 4.31CGWYY53 pKa = 9.97VGGRR57 pKa = 11.84RR58 pKa = 11.84PCPLEE63 pKa = 3.91VSSSEE68 pKa = 4.24TAQTVPKK75 pKa = 10.45AVVQAVLLVLTDD87 pKa = 4.0FYY89 pKa = 11.46EE90 pKa = 4.88RR91 pKa = 11.84RR92 pKa = 11.84TTDD95 pKa = 3.33PDD97 pKa = 3.54VGNTTATNLLDD108 pKa = 3.72QQRR111 pKa = 11.84LGMGII116 pKa = 3.57

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PHZ4|A0A2H4PHZ4_9CAUD Uncharacterized protein OS=Pseudomonas phage Psp6 OX=2053693 GN=Psp6_00026 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.96VMPLRR7 pKa = 11.84LSKK10 pKa = 10.4RR11 pKa = 11.84CVMRR15 pKa = 11.84TFKK18 pKa = 11.06PEE20 pKa = 3.66GATHH24 pKa = 5.57YY25 pKa = 10.61SKK27 pKa = 10.96RR28 pKa = 11.84DD29 pKa = 3.42GMRR32 pKa = 11.84GTLWYY37 pKa = 9.82KK38 pKa = 9.33RR39 pKa = 11.84CEE41 pKa = 4.14RR42 pKa = 11.84TGGWVVWWSAKK53 pKa = 7.13QQWGPSALDD62 pKa = 3.62DD63 pKa = 4.13ASAAVALRR71 pKa = 11.84AVVSDD76 pKa = 4.05TPAIQQ81 pKa = 3.23

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13054

38

910

210.5

23.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.12 ± 0.62

1.509 ± 0.179

5.822 ± 0.235

5.768 ± 0.358

3.363 ± 0.195

8.158 ± 0.315

2.053 ± 0.191

4.673 ± 0.196

5.071 ± 0.307

8.518 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.143

3.654 ± 0.224

5.439 ± 0.407

4.075 ± 0.322

5.707 ± 0.325

5.148 ± 0.225

5.776 ± 0.337

7.109 ± 0.318

2.191 ± 0.159

3.026 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski