Streptomyces sp. PsTaAH-137

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327VTX3|A0A327VTX3_9ACTN NAD(P)-dependent dehydrogenase (Short-subunit alcohol dehydrogenase family) OS=Streptomyces sp. PsTaAH-137 OX=1305830 GN=K377_05529 PE=4 SV=1
MM1 pKa = 7.71AIARR5 pKa = 11.84FGATVLDD12 pKa = 4.53CPQPLALAEE21 pKa = 4.51FYY23 pKa = 11.07AAVLGGTPSVSPDD36 pKa = 2.9EE37 pKa = 4.72SDD39 pKa = 3.18WVDD42 pKa = 3.62LEE44 pKa = 4.49IPGGPALAFQEE55 pKa = 4.4APGYY59 pKa = 8.73VAPPWPSPNGAQQFHH74 pKa = 7.09LDD76 pKa = 3.6VRR78 pKa = 11.84VDD80 pKa = 3.98DD81 pKa = 5.06LDD83 pKa = 3.99AAEE86 pKa = 4.99KK87 pKa = 10.56EE88 pKa = 4.15ILALGATVLDD98 pKa = 4.41NEE100 pKa = 4.77DD101 pKa = 3.77KK102 pKa = 10.75DD103 pKa = 4.39RR104 pKa = 11.84SWRR107 pKa = 11.84VYY109 pKa = 10.64ADD111 pKa = 3.5PVGHH115 pKa = 6.76PFCLCACC122 pKa = 4.94

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327VFY2|A0A327VFY2_9ACTN 50S ribosomal protein L31 OS=Streptomyces sp. PsTaAH-137 OX=1305830 GN=rpmE PE=3 SV=1
MM1 pKa = 7.38SVRR4 pKa = 11.84SRR6 pKa = 11.84PAAVLVLLGALLFGSVPVTAAAHH29 pKa = 5.76TGSTTVSAAASATGHH44 pKa = 5.94TSSAAAPQKK53 pKa = 9.34VTVAKK58 pKa = 10.3SVTRR62 pKa = 11.84IGKK65 pKa = 8.34SKK67 pKa = 9.92RR68 pKa = 11.84RR69 pKa = 11.84HH70 pKa = 5.39KK71 pKa = 10.49SRR73 pKa = 11.84HH74 pKa = 5.07GGSFGKK80 pKa = 10.4VALILIILLVLVVLVVLGLVRR101 pKa = 11.84KK102 pKa = 10.22RR103 pKa = 11.84SRR105 pKa = 11.84SS106 pKa = 3.4

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8133

0

8133

2640280

29

4017

324.6

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.644 ± 0.041

0.781 ± 0.008

6.148 ± 0.022

5.456 ± 0.026

2.784 ± 0.016

9.445 ± 0.027

2.309 ± 0.013

3.165 ± 0.02

2.322 ± 0.026

10.349 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.742 ± 0.01

1.782 ± 0.015

5.954 ± 0.027

2.759 ± 0.017

7.81 ± 0.03

5.123 ± 0.022

6.275 ± 0.023

8.51 ± 0.027

1.529 ± 0.011

2.114 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski