Streptomyces bingchenggensis (strain BCW-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces bingchenggensis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7C2P5|D7C2P5_STRBB Uncharacterized protein OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_02807 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84KK3 pKa = 9.62HH4 pKa = 6.55GIIAATSTLALALGGALFAAPAAQAATTVASVVHH38 pKa = 6.73EE39 pKa = 5.02DD40 pKa = 3.67SEE42 pKa = 4.43LWYY45 pKa = 10.65KK46 pKa = 10.36GAAGQTNNLKK56 pKa = 10.52VSVEE60 pKa = 3.67IEE62 pKa = 3.84QRR64 pKa = 11.84GEE66 pKa = 3.59WEE68 pKa = 4.06SYY70 pKa = 9.23YY71 pKa = 11.04VITFRR76 pKa = 11.84DD77 pKa = 3.49RR78 pKa = 11.84GDD80 pKa = 3.1ITIDD84 pKa = 3.27SSAAEE89 pKa = 4.29YY90 pKa = 10.69DD91 pKa = 3.54EE92 pKa = 4.36CTYY95 pKa = 11.02PSDD98 pKa = 3.85ADD100 pKa = 3.67HH101 pKa = 6.07TVVRR105 pKa = 11.84CATEE109 pKa = 4.11VPLGSDD115 pKa = 3.9DD116 pKa = 4.18SDD118 pKa = 3.97NYY120 pKa = 11.22DD121 pKa = 3.39VNLGDD126 pKa = 4.56GNDD129 pKa = 3.77TATVDD134 pKa = 3.87AGSDD138 pKa = 3.51AYY140 pKa = 10.2STIYY144 pKa = 10.22GGPGDD149 pKa = 5.06DD150 pKa = 3.68VLKK153 pKa = 11.07GSARR157 pKa = 11.84DD158 pKa = 3.59VLRR161 pKa = 11.84GDD163 pKa = 5.04DD164 pKa = 4.14GDD166 pKa = 4.45DD167 pKa = 3.54RR168 pKa = 11.84LDD170 pKa = 3.73GGGGVWGMGPYY181 pKa = 10.13GGPGDD186 pKa = 4.23DD187 pKa = 4.55TITNCSMDD195 pKa = 4.0CYY197 pKa = 10.57GGPGNDD203 pKa = 3.42SMSGGTNDD211 pKa = 4.55TEE213 pKa = 4.32NNMYY217 pKa = 10.85GEE219 pKa = 5.12DD220 pKa = 4.08GNDD223 pKa = 3.68VIHH226 pKa = 6.56GLSDD230 pKa = 3.49ADD232 pKa = 3.91HH233 pKa = 7.08IYY235 pKa = 10.81GGRR238 pKa = 11.84GNDD241 pKa = 3.26TLYY244 pKa = 11.3GEE246 pKa = 4.98EE247 pKa = 4.86GDD249 pKa = 3.73DD250 pKa = 3.69TIYY253 pKa = 11.3GNSGNDD259 pKa = 3.62TIHH262 pKa = 6.54GGKK265 pKa = 8.4GTDD268 pKa = 3.54TLSGGPGTNKK278 pKa = 9.57VYY280 pKa = 10.87QDD282 pKa = 3.16

Molecular weight: 29.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7C9A5|D7C9A5_STRBB Ricin B-type lectin domain-containing protein OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_07503 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.06GRR40 pKa = 11.84GRR42 pKa = 11.84LSAA45 pKa = 4.4

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10019

0

10019

3433066

24

9950

342.7

36.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.686 ± 0.039

0.795 ± 0.007

6.029 ± 0.02

5.638 ± 0.023

2.711 ± 0.012

9.476 ± 0.025

2.371 ± 0.012

3.221 ± 0.017

2.078 ± 0.025

10.34 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.73 ± 0.009

1.764 ± 0.015

6.162 ± 0.024

2.8 ± 0.015

8.16 ± 0.028

5.124 ± 0.018

6.106 ± 0.022

8.155 ± 0.024

1.558 ± 0.01

2.097 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski