Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B) (Eubacterium lentum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella; Eggerthella lenta

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8WIP9|C8WIP9_EGGLE Replicative DNA helicase OS=Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B) OX=479437 GN=Elen_0026 PE=3 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.87RR4 pKa = 11.84EE5 pKa = 4.49VEE7 pKa = 4.15SSDD10 pKa = 3.72VEE12 pKa = 4.29VPEE15 pKa = 4.48IPEE18 pKa = 3.82ILEE21 pKa = 4.07KK22 pKa = 11.1VLLFSLDD29 pKa = 3.36EE30 pKa = 4.68AKK32 pKa = 10.97EE33 pKa = 3.93KK34 pKa = 8.19MTQGSDD40 pKa = 3.53VVPFTALVVKK50 pKa = 10.43EE51 pKa = 3.81NLFIEE56 pKa = 4.18NHH58 pKa = 6.28PADD61 pKa = 3.74SAEE64 pKa = 3.95EE65 pKa = 4.21CFNLARR71 pKa = 11.84HH72 pKa = 5.04TVEE75 pKa = 3.97HH76 pKa = 6.43ARR78 pKa = 11.84GAAAYY83 pKa = 8.28ALCYY87 pKa = 10.17DD88 pKa = 4.68GYY90 pKa = 10.8IEE92 pKa = 5.65IDD94 pKa = 4.06DD95 pKa = 4.26GVKK98 pKa = 10.47DD99 pKa = 3.73ALIAEE104 pKa = 4.68GGVPGEE110 pKa = 4.12DD111 pKa = 2.04TGYY114 pKa = 10.84AVSYY118 pKa = 9.52LYY120 pKa = 11.14EE121 pKa = 4.14MDD123 pKa = 3.65EE124 pKa = 4.29EE125 pKa = 5.09GNVTFEE131 pKa = 4.38EE132 pKa = 4.78EE133 pKa = 3.51PAYY136 pKa = 10.89VGEE139 pKa = 4.86APNFMIALNDD149 pKa = 3.6ADD151 pKa = 4.71SYY153 pKa = 11.43SEE155 pKa = 3.86EE156 pKa = 5.63DD157 pKa = 3.06IDD159 pKa = 4.74EE160 pKa = 4.81KK161 pKa = 11.34YY162 pKa = 11.02LEE164 pKa = 4.41EE165 pKa = 4.87DD166 pKa = 3.41AVDD169 pKa = 4.22DD170 pKa = 4.62EE171 pKa = 4.71EE172 pKa = 4.46

Molecular weight: 19.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8WKC2|C8WKC2_EGGLE Transcriptional regulator AraC family OS=Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B) OX=479437 GN=Elen_2321 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.3AVALVASEE10 pKa = 4.38GSARR14 pKa = 11.84ASEE17 pKa = 4.11ARR19 pKa = 11.84RR20 pKa = 11.84ALRR23 pKa = 11.84LDD25 pKa = 3.39PRR27 pKa = 11.84TKK29 pKa = 11.02VLMLVCANVTLLCSGFDD46 pKa = 3.2AAGFVLKK53 pKa = 10.18FLVAGIVVALLIAAGRR69 pKa = 11.84RR70 pKa = 11.84AAGIGFAAVFAAAALLEE87 pKa = 4.03QMNEE91 pKa = 3.87RR92 pKa = 11.84GLLDD96 pKa = 3.85ALGSTSAAAVTLRR109 pKa = 11.84FLSALVLQLMPGTMFAYY126 pKa = 10.68YY127 pKa = 10.6LFATTKK133 pKa = 9.9VSEE136 pKa = 4.68FVAAMEE142 pKa = 4.51RR143 pKa = 11.84VRR145 pKa = 11.84LPQRR149 pKa = 11.84VIIPFAVVFRR159 pKa = 11.84FFPTVLEE166 pKa = 4.43EE167 pKa = 3.87YY168 pKa = 10.52RR169 pKa = 11.84SIRR172 pKa = 11.84DD173 pKa = 3.36AMRR176 pKa = 11.84LRR178 pKa = 11.84GVGWRR183 pKa = 11.84SGPVALVEE191 pKa = 4.07YY192 pKa = 10.45RR193 pKa = 11.84LVPLVAGMVKK203 pKa = 10.04IGDD206 pKa = 4.04EE207 pKa = 4.07LSAASVTRR215 pKa = 11.84GLGGEE220 pKa = 4.04AVRR223 pKa = 11.84TSRR226 pKa = 11.84CRR228 pKa = 11.84IGFAAADD235 pKa = 3.52AALATVFLACAAATAAKK252 pKa = 10.66GLMGWW257 pKa = 3.62

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

1043249

30

2099

341.6

37.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.314 ± 0.06

1.59 ± 0.02

6.194 ± 0.039

6.506 ± 0.046

3.925 ± 0.029

8.297 ± 0.042

1.709 ± 0.018

4.747 ± 0.033

3.668 ± 0.034

9.396 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.021

2.754 ± 0.027

4.369 ± 0.029

2.809 ± 0.024

6.047 ± 0.053

5.748 ± 0.037

5.08 ± 0.033

8.226 ± 0.042

1.127 ± 0.017

2.832 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski