Hubei tombus-like virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGK2|A0A1L3KGK2_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 20 OX=1923267 PE=4 SV=1
MM1 pKa = 7.47VKK3 pKa = 10.38DD4 pKa = 4.26CLPTTDD10 pKa = 4.66CGAHH14 pKa = 5.06YY15 pKa = 9.62VQEE18 pKa = 4.4VVAPSGATSCDD29 pKa = 3.47GPPDD33 pKa = 4.24GSDD36 pKa = 3.2QSSSPAKK43 pKa = 10.04TRR45 pKa = 11.84DD46 pKa = 3.18QVIIPRR52 pKa = 11.84PSWIDD57 pKa = 3.39GTWDD61 pKa = 3.2VVTFLTPYY69 pKa = 9.85YY70 pKa = 8.29VQQVVSIAFPSEE82 pKa = 3.4ISATTVRR89 pKa = 11.84PYY91 pKa = 11.18ARR93 pKa = 11.84WVINSINTQFWWDD106 pKa = 3.58TQLPQWINPAEE117 pKa = 3.91HH118 pKa = 6.8VIYY121 pKa = 10.42NDD123 pKa = 2.84QGEE126 pKa = 4.5IVLVPFPFAVGFHH139 pKa = 6.72CPRR142 pKa = 11.84LLRR145 pKa = 11.84PLFEE149 pKa = 4.77GLGPEE154 pKa = 4.06PRR156 pKa = 11.84ILTEE160 pKa = 3.76TRR162 pKa = 11.84EE163 pKa = 3.79MRR165 pKa = 11.84RR166 pKa = 11.84WGRR169 pKa = 11.84YY170 pKa = 5.53ITITPVANATEE181 pKa = 4.48FKK183 pKa = 10.88GRR185 pKa = 11.84IAAASLQFNSALNPVHH201 pKa = 7.17AMVEE205 pKa = 4.36GGPDD209 pKa = 3.73PGPGPVDD216 pKa = 3.41PTPPPTLEE224 pKa = 3.95WARR227 pKa = 11.84FWCDD231 pKa = 2.81NYY233 pKa = 11.4SGTNPYY239 pKa = 10.55LYY241 pKa = 10.05TRR243 pKa = 11.84AGLTRR248 pKa = 11.84TWWSSSWLFEE258 pKa = 5.02DD259 pKa = 4.56NLSFDD264 pKa = 4.57LFNLNGNIIIPGNTPFQTSIVVLTSGGAPPALSFHH299 pKa = 4.75VTFRR303 pKa = 11.84VGNNVVFQGDD313 pKa = 4.12FSPGVAIPLFKK324 pKa = 10.43GRR326 pKa = 11.84RR327 pKa = 11.84AIRR330 pKa = 11.84STPIDD335 pKa = 3.45TDD337 pKa = 3.15EE338 pKa = 4.25LARR341 pKa = 11.84YY342 pKa = 6.94TITPEE347 pKa = 3.92FSFEE351 pKa = 4.08ALLQADD357 pKa = 4.13EE358 pKa = 4.85KK359 pKa = 11.16SYY361 pKa = 10.7SNNWIEE367 pKa = 4.43GAHH370 pKa = 6.4SRR372 pKa = 11.84QAWGKK377 pKa = 9.82GYY379 pKa = 11.04LEE381 pKa = 4.03FTPTRR386 pKa = 11.84EE387 pKa = 3.61WRR389 pKa = 11.84PLIRR393 pKa = 11.84APAGGRR399 pKa = 11.84VVIDD403 pKa = 3.24QASIKK408 pKa = 10.14RR409 pKa = 11.84DD410 pKa = 3.34IVDD413 pKa = 5.24LSGKK417 pKa = 7.38WHH419 pKa = 5.34VTYY422 pKa = 10.86ASNIDD427 pKa = 3.58PKK429 pKa = 11.13ASYY432 pKa = 10.25TMMYY436 pKa = 7.56GTHH439 pKa = 5.76YY440 pKa = 10.17QLCNEE445 pKa = 4.3TDD447 pKa = 3.38ASFQLFKK454 pKa = 11.06KK455 pKa = 9.7PVPARR460 pKa = 11.84DD461 pKa = 3.54DD462 pKa = 3.9GAIEE466 pKa = 3.98MALTLQTALPHH477 pKa = 5.76TFPAKK482 pKa = 10.54FNDD485 pKa = 3.92GGILPMILSQVKK497 pKa = 9.26RR498 pKa = 11.84VGSAGLAGLAKK509 pKa = 10.42GIVGSLMGGLQTIGTADD526 pKa = 3.47RR527 pKa = 11.84VIGYY531 pKa = 8.8PSMSTTYY538 pKa = 10.93GNNPVSLYY546 pKa = 10.78GGNGKK551 pKa = 8.33TNGRR555 pKa = 11.84RR556 pKa = 11.84GRR558 pKa = 11.84KK559 pKa = 8.84KK560 pKa = 10.01RR561 pKa = 11.84ANGRR565 pKa = 11.84DD566 pKa = 3.55AIASLMSRR574 pKa = 11.84TSNII578 pKa = 3.07

Molecular weight: 63.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGK2|A0A1L3KGK2_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 20 OX=1923267 PE=4 SV=1
MM1 pKa = 7.71NDD3 pKa = 3.03RR4 pKa = 11.84GNRR7 pKa = 11.84VPLGGKK13 pKa = 8.69RR14 pKa = 11.84PKK16 pKa = 8.23MTLAQVVAKK25 pKa = 8.16FAPYY29 pKa = 10.51LKK31 pKa = 10.17PRR33 pKa = 11.84VDD35 pKa = 2.99ALQRR39 pKa = 11.84TEE41 pKa = 4.19RR42 pKa = 11.84LYY44 pKa = 11.23KK45 pKa = 10.34EE46 pKa = 5.24LMAKK50 pKa = 8.49TPPHH54 pKa = 5.93SAVKK58 pKa = 9.15KK59 pKa = 9.53QRR61 pKa = 11.84VATRR65 pKa = 11.84PNKK68 pKa = 10.37CYY70 pKa = 10.37EE71 pKa = 4.56CGSKK75 pKa = 7.33QHH77 pKa = 5.87RR78 pKa = 11.84TYY80 pKa = 10.54GTDD83 pKa = 3.1RR84 pKa = 11.84EE85 pKa = 4.43GWVACEE91 pKa = 4.38CFRR94 pKa = 11.84CGTEE98 pKa = 4.18YY99 pKa = 10.42FYY101 pKa = 11.19HH102 pKa = 7.15PNQGVQSSAGYY113 pKa = 7.41TAIGTKK119 pKa = 10.13PVVNNSASWAGVVKK133 pKa = 10.61SNKK136 pKa = 9.4RR137 pKa = 11.84PQTPSSDD144 pKa = 2.97WKK146 pKa = 10.95KK147 pKa = 10.87DD148 pKa = 3.2WNKK151 pKa = 10.05VKK153 pKa = 10.84DD154 pKa = 3.76KK155 pKa = 11.16VSNQTTTATPQAPQSPQPPKK175 pKa = 10.45LSRR178 pKa = 11.84AQRR181 pKa = 11.84AKK183 pKa = 10.17RR184 pKa = 11.84AKK186 pKa = 9.25EE187 pKa = 3.54LRR189 pKa = 11.84AEE191 pKa = 4.2KK192 pKa = 10.1LAKK195 pKa = 8.62QAKK198 pKa = 9.6PITQLKK204 pKa = 9.95SSSEE208 pKa = 4.32SPDD211 pKa = 3.04TALDD215 pKa = 3.44VTEE218 pKa = 4.46IKK220 pKa = 10.0EE221 pKa = 4.04DD222 pKa = 3.57KK223 pKa = 9.65PIKK226 pKa = 10.46VNGEE230 pKa = 3.97IITAEE235 pKa = 4.07ALTASINQVLKK246 pKa = 10.01TVDD249 pKa = 3.46VSDD252 pKa = 5.12DD253 pKa = 3.65LPTYY257 pKa = 8.91EE258 pKa = 5.37QYY260 pKa = 11.39LKK262 pKa = 10.9DD263 pKa = 4.21FRR265 pKa = 11.84TEE267 pKa = 3.67SHH269 pKa = 6.04KK270 pKa = 11.05QPGEE274 pKa = 3.7VGKK277 pKa = 9.34WIKK280 pKa = 10.45SAEE283 pKa = 4.29QILQSWPTRR292 pKa = 11.84LLVCTPTGVYY302 pKa = 9.15RR303 pKa = 11.84VKK305 pKa = 10.69RR306 pKa = 11.84DD307 pKa = 3.51LEE309 pKa = 4.25STKK312 pKa = 10.43PYY314 pKa = 10.39LLSYY318 pKa = 10.9LKK320 pKa = 10.55LLKK323 pKa = 10.63AADD326 pKa = 4.55LEE328 pKa = 5.1DD329 pKa = 5.62LDD331 pKa = 5.31LSNWDD336 pKa = 3.67QQISAAAMMVATKK349 pKa = 9.72PLRR352 pKa = 11.84ASWRR356 pKa = 11.84RR357 pKa = 11.84AKK359 pKa = 10.3GRR361 pKa = 11.84RR362 pKa = 11.84KK363 pKa = 8.56TGGTNPPTRR372 pKa = 11.84EE373 pKa = 4.04CKK375 pKa = 10.02EE376 pKa = 4.02SSKK379 pKa = 11.18EE380 pKa = 3.81MAGTT384 pKa = 3.52

Molecular weight: 43.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1768

336

578

442.0

49.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.749 ± 0.685

1.867 ± 0.378

4.864 ± 0.124

4.921 ± 0.566

3.45 ± 0.647

7.24 ± 0.683

1.867 ± 0.296

5.09 ± 0.634

6.335 ± 1.341

7.862 ± 0.567

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.376 ± 0.393

4.186 ± 0.643

6.731 ± 0.619

3.846 ± 0.417

6.505 ± 0.181

6.787 ± 0.235

6.505 ± 0.771

6.165 ± 0.176

2.036 ± 0.337

3.62 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski