Roseburia sp. CAG:50

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5U4L6|R5U4L6_9FIRM Sugar ABC transporter permease protein OS=Roseburia sp. CAG:50 OX=1262949 GN=BN683_01107 PE=3 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84AFILVILSLILGISGNRR19 pKa = 11.84QLANQFSMANMSQTLQVALAQGIMIEE45 pKa = 4.69NIDD48 pKa = 3.59THH50 pKa = 8.11GGFHH54 pKa = 7.69GDD56 pKa = 3.72GQSLTVWKK64 pKa = 10.39FDD66 pKa = 4.24DD67 pKa = 4.33NSILEE72 pKa = 4.42QILTDD77 pKa = 4.42PDD79 pKa = 3.54WKK81 pKa = 10.3EE82 pKa = 4.72LPMTDD87 pKa = 3.25NLEE90 pKa = 4.07ALLYY94 pKa = 10.56GVVYY98 pKa = 8.82DD99 pKa = 4.0TGLSITEE106 pKa = 4.41IGPCVDD112 pKa = 4.69FSEE115 pKa = 4.45EE116 pKa = 3.95QLPQIQNGYY125 pKa = 9.3YY126 pKa = 10.13YY127 pKa = 10.63FVDD130 pKa = 3.76RR131 pKa = 11.84QAEE134 pKa = 4.19SEE136 pKa = 4.24MQHH139 pKa = 7.21SDD141 pKa = 3.08AQLMEE146 pKa = 4.28RR147 pKa = 11.84ASLNFSIALYY157 pKa = 10.72DD158 pKa = 3.46VDD160 pKa = 5.09TDD162 pKa = 3.45TLYY165 pKa = 11.25YY166 pKa = 10.19IEE168 pKa = 5.02VDD170 pKa = 3.5TT171 pKa = 5.51

Molecular weight: 19.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5TXL7|R5TXL7_9FIRM L-fucose isomerase OS=Roseburia sp. CAG:50 OX=1262949 GN=BN683_00850 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.1KK9 pKa = 7.57RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.33VHH16 pKa = 5.63GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.6GRR39 pKa = 11.84KK40 pKa = 9.07KK41 pKa = 9.61LTAA44 pKa = 4.2

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2482

0

2482

807152

29

2028

325.2

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.816 ± 0.052

1.633 ± 0.021

5.714 ± 0.042

7.463 ± 0.056

3.953 ± 0.035

7.14 ± 0.045

1.946 ± 0.024

7.187 ± 0.051

6.407 ± 0.034

8.704 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.23 ± 0.027

4.292 ± 0.039

3.292 ± 0.029

3.704 ± 0.032

4.232 ± 0.039

5.522 ± 0.035

5.602 ± 0.044

7.047 ± 0.039

0.953 ± 0.022

4.157 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski