Bacillus virus AP50

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Betatectivirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6RT46|B6RT46_9VIRU DNA packaging ATPase OS=Bacillus virus AP50 OX=513550 PE=4 SV=1
MM1 pKa = 7.96LDD3 pKa = 3.45QIQIPNEE10 pKa = 3.98NKK12 pKa = 9.58EE13 pKa = 4.44SKK15 pKa = 9.58QEE17 pKa = 3.86RR18 pKa = 11.84LQLVVDD24 pKa = 3.79QVVDD28 pKa = 3.74SLPTTVRR35 pKa = 11.84LLAGSYY41 pKa = 10.45LNSFRR46 pKa = 11.84QVLEE50 pKa = 4.32SEE52 pKa = 4.17QHH54 pKa = 7.05DD55 pKa = 3.29IDD57 pKa = 4.84GNIDD61 pKa = 3.54LALSRR66 pKa = 11.84LRR68 pKa = 11.84EE69 pKa = 4.19YY70 pKa = 10.41IDD72 pKa = 3.81YY73 pKa = 10.24IQYY76 pKa = 10.89GHH78 pKa = 6.39DD79 pKa = 3.6QEE81 pKa = 5.88NEE83 pKa = 3.7

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6RT55|B6RT55_9VIRU Minor capsid protein OS=Bacillus virus AP50 OX=513550 PE=4 SV=1
MM1 pKa = 7.22SRR3 pKa = 11.84VLGIVSGIGMLIAVYY18 pKa = 10.52LFLNNAKK25 pKa = 8.46QTTQIIDD32 pKa = 3.81SIAGNGVKK40 pKa = 10.26GIKK43 pKa = 8.69TLQGRR48 pKa = 3.88

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

4590

29

731

148.1

16.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.013 ± 0.535

0.654 ± 0.173

5.948 ± 0.49

6.885 ± 0.829

4.532 ± 0.333

7.32 ± 0.647

1.656 ± 0.248

5.817 ± 0.358

8.606 ± 0.62

8.279 ± 0.511

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.29 ± 0.303

5.098 ± 0.374

3.682 ± 0.442

4.248 ± 0.415

4.052 ± 0.286

6.122 ± 0.551

6.144 ± 0.414

6.972 ± 0.443

0.98 ± 0.158

3.704 ± 0.541
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski