Streptomyces sp. CB02056

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4WK48|A0A1Q4WK48_9ACTN Uncharacterized protein OS=Streptomyces sp. CB02056 OX=1703924 GN=AMK13_04920 PE=4 SV=1
MM1 pKa = 7.49PVVTDD6 pKa = 4.33PYY8 pKa = 11.32KK9 pKa = 10.72PGTPCWVDD17 pKa = 4.55LMASDD22 pKa = 3.85QQAALDD28 pKa = 4.19FYY30 pKa = 11.32RR31 pKa = 11.84DD32 pKa = 3.38LFGWQGEE39 pKa = 4.34VGPAEE44 pKa = 3.96FGGYY48 pKa = 8.19AVCMLNGRR56 pKa = 11.84PVAGIMAQAAPEE68 pKa = 4.27GQPTPPVAWTTYY80 pKa = 9.83LASDD84 pKa = 4.1DD85 pKa = 4.33ADD87 pKa = 3.43AARR90 pKa = 11.84ASITEE95 pKa = 3.82AGGTVLYY102 pKa = 10.96DD103 pKa = 3.52PMDD106 pKa = 3.65VGTIGRR112 pKa = 11.84MLVAADD118 pKa = 3.31PTGAVFGVWQAMDD131 pKa = 4.24FIGAGVVNEE140 pKa = 4.37PGALVWNEE148 pKa = 4.27LNTADD153 pKa = 3.62TGAAGRR159 pKa = 11.84FYY161 pKa = 11.0QPALGLRR168 pKa = 11.84PATIQGMDD176 pKa = 3.61GYY178 pKa = 11.1FSLNVADD185 pKa = 4.06RR186 pKa = 11.84TVGGMQAIPGYY197 pKa = 9.62LAAGTPSHH205 pKa = 5.93WMTYY209 pKa = 9.86FSVDD213 pKa = 3.45GADD216 pKa = 3.47STVDD220 pKa = 3.51ALVKK224 pKa = 10.78AGGSVIQPAFDD235 pKa = 3.62MQSGRR240 pKa = 11.84MAVVQDD246 pKa = 3.52PQGAVFAVIEE256 pKa = 4.28APEE259 pKa = 4.0AQMPDD264 pKa = 3.41SPP266 pKa = 4.78

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4W9W1|A0A1Q4W9W1_9ACTN Uncharacterized protein OS=Streptomyces sp. CB02056 OX=1703924 GN=AMK13_15825 PE=4 SV=1
MM1 pKa = 7.49VPGRR5 pKa = 11.84PAAGAVTPPPPLPAVPVRR23 pKa = 11.84PPGRR27 pKa = 11.84GTRR30 pKa = 11.84HH31 pKa = 5.53RR32 pKa = 11.84CGPTVGPPGAHH43 pKa = 6.84PSSARR48 pKa = 11.84RR49 pKa = 11.84PGPARR54 pKa = 11.84PHH56 pKa = 5.68PRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84PRR63 pKa = 11.84VVPRR67 pKa = 11.84RR68 pKa = 11.84LNPVRR73 pKa = 11.84PLPAGVGPPRR83 pKa = 11.84ARR85 pKa = 11.84LFSAFRR91 pKa = 11.84PRR93 pKa = 11.84RR94 pKa = 11.84LPVVPRR100 pKa = 11.84QPRR103 pKa = 11.84PVRR106 pKa = 11.84PPPAGARR113 pKa = 11.84PSRR116 pKa = 11.84ARR118 pKa = 11.84PSSVRR123 pKa = 11.84RR124 pKa = 11.84PRR126 pKa = 11.84PRR128 pKa = 11.84RR129 pKa = 11.84QHH131 pKa = 6.32RR132 pKa = 11.84FPVVPRR138 pKa = 11.84RR139 pKa = 11.84LSLTRR144 pKa = 11.84PPPVGAGPSRR154 pKa = 11.84APRR157 pKa = 11.84PRR159 pKa = 11.84PRR161 pKa = 11.84RR162 pKa = 11.84QRR164 pKa = 11.84RR165 pKa = 11.84PNSTRR170 pKa = 11.84PLPAGAGPPRR180 pKa = 11.84ARR182 pKa = 11.84PSSVRR187 pKa = 11.84RR188 pKa = 11.84PRR190 pKa = 11.84SRR192 pKa = 11.84RR193 pKa = 11.84QRR195 pKa = 11.84RR196 pKa = 11.84PSPTRR201 pKa = 11.84PLPGRR206 pKa = 11.84AGAWARR212 pKa = 11.84RR213 pKa = 11.84STRR216 pKa = 11.84CRR218 pKa = 11.84RR219 pKa = 11.84RR220 pKa = 11.84PPWRR224 pKa = 11.84GAPLPCSAGADD235 pKa = 3.3RR236 pKa = 11.84SSGSRR241 pKa = 11.84LRR243 pKa = 11.84RR244 pKa = 11.84QARR247 pKa = 11.84PSLPSPRR254 pKa = 11.84RR255 pKa = 3.4

Molecular weight: 28.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7263

3

7266

2401140

36

9192

330.5

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.379 ± 0.052

0.767 ± 0.008

5.834 ± 0.022

5.591 ± 0.029

2.659 ± 0.016

9.712 ± 0.028

2.269 ± 0.014

2.821 ± 0.022

1.798 ± 0.025

10.887 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.532 ± 0.013

1.698 ± 0.016

6.447 ± 0.032

2.745 ± 0.017

8.116 ± 0.032

4.713 ± 0.021

6.05 ± 0.025

8.455 ± 0.026

1.51 ± 0.012

2.017 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski