Dokdonia pacifica

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Dokdonia

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238VLM1|A0A238VLM1_9FLAO FixH protein OS=Dokdonia pacifica OX=1627892 GN=SAMN06265376_10118 PE=4 SV=1
MM1 pKa = 7.06KK2 pKa = 9.7TITYY6 pKa = 10.17RR7 pKa = 11.84RR8 pKa = 11.84ITILLRR14 pKa = 11.84LFTFFLLFPFTIHH27 pKa = 5.68ATIEE31 pKa = 4.02RR32 pKa = 11.84EE33 pKa = 3.89EE34 pKa = 4.15NNISFACTGTVIDD47 pKa = 4.11TFPYY51 pKa = 9.55TEE53 pKa = 4.26SFEE56 pKa = 4.3TGNFDD61 pKa = 3.77FTQNTDD67 pKa = 4.57DD68 pKa = 5.0DD69 pKa = 5.3GDD71 pKa = 3.63WTRR74 pKa = 11.84DD75 pKa = 3.64NNGTPSNNTGPDD87 pKa = 3.49DD88 pKa = 4.97ASDD91 pKa = 3.64GNFYY95 pKa = 11.08LFTEE99 pKa = 5.27ASTSGQGAMGFNASAILTSPCFDD122 pKa = 3.38FTGVGGATFNFDD134 pKa = 2.91YY135 pKa = 11.05HH136 pKa = 6.43MYY138 pKa = 10.71GNAIGQISVEE148 pKa = 4.1VSDD151 pKa = 5.41DD152 pKa = 4.14GGVNWNVLQVISGQQQTSGSAPWITSSINLNAYY185 pKa = 8.92VGNTINVRR193 pKa = 11.84IVGTTGSSFSGDD205 pKa = 3.21MAIDD209 pKa = 3.67NVEE212 pKa = 4.17VTVTPVNPEE221 pKa = 3.52INITGLGNDD230 pKa = 3.55IVSGDD235 pKa = 3.77VTPTVADD242 pKa = 3.7GTDD245 pKa = 3.63FGDD248 pKa = 3.38ITLGLSNSSTFFIEE262 pKa = 3.7NSGILEE268 pKa = 4.68LLLTDD273 pKa = 4.59PNPYY277 pKa = 8.52ITITGTNAGDD287 pKa = 3.43FSIATIPTTPIAPVNGSTPFAIGFTPSAIGDD318 pKa = 3.84RR319 pKa = 11.84FATISIASNDD329 pKa = 3.63SDD331 pKa = 3.71EE332 pKa = 4.37NPYY335 pKa = 10.72VFDD338 pKa = 3.73IMGTGVAPSSEE349 pKa = 4.1INVSGLGNAIMSGDD363 pKa = 4.05VIPSVFDD370 pKa = 3.24GTDD373 pKa = 3.43FGFTTLGTSVSSDD386 pKa = 3.08FVIINSGLLGLDD398 pKa = 3.41LTNASPYY405 pKa = 10.56VNLIGADD412 pKa = 3.24ASQFSISVIPNNTISNNGGTTQFQITYY439 pKa = 8.62TPVSVGVHH447 pKa = 4.93TVTVSIVNNDD457 pKa = 3.37PDD459 pKa = 3.87EE460 pKa = 4.63NPFVFDD466 pKa = 3.18ITGEE470 pKa = 4.05AVVTFVPEE478 pKa = 3.87IEE480 pKa = 4.08ITGNAVEE487 pKa = 4.64IVNGDD492 pKa = 4.24MIPDD496 pKa = 3.37VSDD499 pKa = 3.14ATDD502 pKa = 3.23YY503 pKa = 9.79GTISLGTKK511 pKa = 9.74KK512 pKa = 10.35NVAFTINNVGSGALTLSGPPPYY534 pKa = 9.84LTITGTDD541 pKa = 3.01ASQFSIQTEE550 pKa = 4.09PSSSIAASAATVFQIQYY567 pKa = 10.28NPFGLGTHH575 pKa = 6.35TATVTVVSDD584 pKa = 5.2DD585 pKa = 3.66SDD587 pKa = 3.56EE588 pKa = 4.49PNYY591 pKa = 11.14SFTITGTAIEE601 pKa = 4.55NEE603 pKa = 4.37NPLEE607 pKa = 4.12TPYY610 pKa = 10.86YY611 pKa = 11.11ANFDD615 pKa = 3.78TSDD618 pKa = 4.1DD619 pKa = 3.41NWVASNPGGGSIWTHH634 pKa = 4.39GTNAVEE640 pKa = 4.51VGTEE644 pKa = 3.45GDD646 pKa = 3.11YY647 pKa = 10.48WYY649 pKa = 9.77TDD651 pKa = 2.99TYY653 pKa = 11.75DD654 pKa = 4.97DD655 pKa = 4.12YY656 pKa = 12.04ASNSDD661 pKa = 3.81TYY663 pKa = 9.51VTSPIIDD670 pKa = 3.43LSGYY674 pKa = 10.28QNLTFKK680 pKa = 10.58IDD682 pKa = 2.9IRR684 pKa = 11.84YY685 pKa = 7.86DD686 pKa = 3.33TDD688 pKa = 3.77GDD690 pKa = 3.84TGGDD694 pKa = 3.06DD695 pKa = 4.04GMNIEE700 pKa = 4.34YY701 pKa = 10.68SPDD704 pKa = 3.33GGATWFVLGAYY715 pKa = 9.73ADD717 pKa = 3.85PQVDD721 pKa = 2.69NWYY724 pKa = 10.89NGDD727 pKa = 3.76NVDD730 pKa = 4.48ALGNGIDD737 pKa = 3.5GWAGFSFDD745 pKa = 3.94IVSATKK751 pKa = 10.45SDD753 pKa = 4.9FIQASVDD760 pKa = 3.83LPVEE764 pKa = 4.22LVNNPVARR772 pKa = 11.84FRR774 pKa = 11.84VHH776 pKa = 6.58FASDD780 pKa = 2.98NSQNDD785 pKa = 3.08NGVNFDD791 pKa = 3.64NVFVLGDD798 pKa = 4.6PIVSDD803 pKa = 5.0DD804 pKa = 4.75PIEE807 pKa = 4.68GPAGVYY813 pKa = 9.7TNLKK817 pKa = 9.49LWLKK821 pKa = 6.05TTEE824 pKa = 4.4GVGGASNAGQLAAWEE839 pKa = 4.28DD840 pKa = 3.57QALDD844 pKa = 3.69NNATRR849 pKa = 11.84GDD851 pKa = 3.75SGQQPIYY858 pKa = 10.68YY859 pKa = 10.2DD860 pKa = 5.03DD861 pKa = 3.58INEE864 pKa = 4.54NINYY868 pKa = 9.62NPVVSFNDD876 pKa = 3.83VIDD879 pKa = 4.39SEE881 pKa = 5.03LKK883 pKa = 10.75GKK885 pKa = 10.66GGFNTQDD892 pKa = 2.96YY893 pKa = 8.73WIVMQADD900 pKa = 4.08GSVNSSSPIKK910 pKa = 10.48GVIAGRR916 pKa = 11.84VSRR919 pKa = 11.84DD920 pKa = 3.21QFSEE924 pKa = 4.23DD925 pKa = 4.13GTGLWINPGSLRR937 pKa = 11.84FSGVDD942 pKa = 3.2NIVSHH947 pKa = 5.66MVGATSASASDD958 pKa = 3.96IGDD961 pKa = 3.39GSYY964 pKa = 11.06GRR966 pKa = 11.84AYY968 pKa = 9.74TSSTDD973 pKa = 3.25SYY975 pKa = 11.29DD976 pKa = 3.54NEE978 pKa = 4.64PIILNVKK985 pKa = 9.1VNNAGDD991 pKa = 3.3QTEE994 pKa = 4.29IYY996 pKa = 10.12KK997 pKa = 10.79NGIRR1001 pKa = 11.84IDD1003 pKa = 3.65NYY1005 pKa = 8.97TGKK1008 pKa = 7.46TTGSPSEE1015 pKa = 4.32DD1016 pKa = 2.97LPYY1019 pKa = 10.66INVTNSSYY1027 pKa = 10.92ILGVGRR1033 pKa = 11.84ITVGGTNYY1041 pKa = 10.39DD1042 pKa = 3.34SHH1044 pKa = 8.45FNGKK1048 pKa = 7.78MSEE1051 pKa = 4.35VISYY1055 pKa = 10.41ANPLTIFDD1063 pKa = 3.7QEE1065 pKa = 4.6KK1066 pKa = 8.35IQSYY1070 pKa = 9.8LAIKK1074 pKa = 10.11YY1075 pKa = 9.57GITLHH1080 pKa = 6.53DD1081 pKa = 4.17TNSTSITRR1089 pKa = 11.84LGDD1092 pKa = 3.22QNYY1095 pKa = 9.32IDD1097 pKa = 4.89SDD1099 pKa = 4.03GSTIWNVAAHH1109 pKa = 6.36TGYY1112 pKa = 10.84NYY1114 pKa = 10.53DD1115 pKa = 2.96IAGIGRR1121 pKa = 11.84DD1122 pKa = 3.16ITSGLNQKK1130 pKa = 9.85QSISVNSGAIVTMGLTEE1147 pKa = 4.13VYY1149 pKa = 8.46NTNNEE1154 pKa = 4.16NIANNTNTIVDD1165 pKa = 4.28KK1166 pKa = 11.44NFLMWGNDD1174 pKa = 3.3NKK1176 pKa = 11.0SLNAAASIIVDD1187 pKa = 4.53LSDD1190 pKa = 5.34GIAGLNTVVDD1200 pKa = 3.69ITSVEE1205 pKa = 4.18RR1206 pKa = 11.84SWKK1209 pKa = 9.41VVEE1212 pKa = 4.12TGTVGRR1218 pKa = 11.84VKK1220 pKa = 10.89VSVPEE1225 pKa = 3.94IALSATLDD1233 pKa = 3.66PPGSFFMFISDD1244 pKa = 3.79TPTFNPSSEE1253 pKa = 3.89YY1254 pKa = 10.64RR1255 pKa = 11.84IMLPNGDD1262 pKa = 3.91NVEE1265 pKa = 4.1AEE1267 pKa = 4.16YY1268 pKa = 11.15DD1269 pKa = 3.8FEE1271 pKa = 4.25GTKK1274 pKa = 10.86YY1275 pKa = 9.06ITFGYY1280 pKa = 10.05APDD1283 pKa = 3.76YY1284 pKa = 11.34VFDD1287 pKa = 5.01RR1288 pKa = 11.84SITFNGTTDD1297 pKa = 3.29YY1298 pKa = 11.07MDD1300 pKa = 3.75AQDD1303 pKa = 4.03YY1304 pKa = 11.35LDD1306 pKa = 4.07IEE1308 pKa = 4.79GDD1310 pKa = 3.84FTISTWIKK1318 pKa = 10.41HH1319 pKa = 5.14SSQDD1323 pKa = 3.47YY1324 pKa = 8.8TVLSKK1329 pKa = 11.11NDD1331 pKa = 3.53PAFNEE1336 pKa = 4.91GYY1338 pKa = 9.24EE1339 pKa = 4.16LKK1341 pKa = 10.61INTDD1345 pKa = 3.44RR1346 pKa = 11.84TVSMIWKK1353 pKa = 9.53NGSTQSITSSTQIPNNEE1370 pKa = 3.43WHH1372 pKa = 6.55QIAVSYY1378 pKa = 10.59DD1379 pKa = 3.11GSSASLYY1386 pKa = 9.96IDD1388 pKa = 3.61GVLDD1392 pKa = 3.52VQAPLTAPVTNNEE1405 pKa = 4.14KK1406 pKa = 10.55FLIAAGRR1413 pKa = 11.84GSTPADD1419 pKa = 4.0FYY1421 pKa = 11.25EE1422 pKa = 4.34GTIDD1426 pKa = 4.95EE1427 pKa = 4.35VRR1429 pKa = 11.84VWDD1432 pKa = 4.06AALTANQIRR1441 pKa = 11.84FIMNQEE1447 pKa = 3.53ILEE1450 pKa = 4.25NTNLMVMGEE1459 pKa = 4.91IIPATISKK1467 pKa = 10.48NDD1469 pKa = 3.1IDD1471 pKa = 5.18AIPWDD1476 pKa = 3.88SLDD1479 pKa = 4.14GYY1481 pKa = 11.5FPMTTYY1487 pKa = 11.53AFTNVKK1493 pKa = 9.73GASNNTVVAAIKK1505 pKa = 10.18NLKK1508 pKa = 8.42TVDD1511 pKa = 4.07FQTAPLPYY1519 pKa = 9.89VSQANGNWTNPATWEE1534 pKa = 4.2NGTGFQIPNATSIVDD1549 pKa = 3.27NTVRR1553 pKa = 11.84VDD1555 pKa = 3.13WNIVQTAHH1563 pKa = 6.9NVTTQEE1569 pKa = 3.99NNTVLALDD1577 pKa = 3.75VQTDD1581 pKa = 3.69EE1582 pKa = 5.9LSIEE1586 pKa = 4.07NDD1588 pKa = 3.22SKK1590 pKa = 11.42IEE1592 pKa = 3.8VSHH1595 pKa = 5.33YY1596 pKa = 10.95LKK1598 pKa = 10.85LDD1600 pKa = 3.52GVLDD1604 pKa = 4.01LVGEE1608 pKa = 4.32SQLIQTEE1615 pKa = 4.05NSDD1618 pKa = 4.45LDD1620 pKa = 3.75ATSTGSLEE1628 pKa = 4.22KK1629 pKa = 10.51DD1630 pKa = 3.36QQGTADD1636 pKa = 3.6TYY1638 pKa = 11.63SYY1640 pKa = 10.4NIWAAPVSTINGTQINTNYY1659 pKa = 9.27TVASMMKK1666 pKa = 10.48DD1667 pKa = 2.88GTNPNNPLNMSFTGGLNGAATTPITISSFWMFKK1700 pKa = 9.83YY1701 pKa = 10.91GNGIEE1706 pKa = 4.08QFQPIGSTGNVSVGEE1721 pKa = 4.61GYY1723 pKa = 8.77TMKK1726 pKa = 10.8GPGSGAITDD1735 pKa = 3.65PQNYY1739 pKa = 8.8VFVGKK1744 pKa = 9.59PNNSTDD1750 pKa = 3.38AEE1752 pKa = 4.4EE1753 pKa = 5.14IIVPAIADD1761 pKa = 3.87RR1762 pKa = 11.84DD1763 pKa = 4.12YY1764 pKa = 11.15MVGNPFPSALDD1775 pKa = 3.6ANDD1778 pKa = 5.16FINDD1782 pKa = 3.64NPHH1785 pKa = 7.25LDD1787 pKa = 3.31GTLYY1791 pKa = 10.74FF1792 pKa = 4.81

Molecular weight: 192.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239C1Q6|A0A239C1Q6_9FLAO Pimeloyl-ACP methyl ester carboxylesterase OS=Dokdonia pacifica OX=1627892 GN=SAMN06265376_10741 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.19RR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.52LSVSSEE48 pKa = 3.87PRR50 pKa = 11.84HH51 pKa = 5.92KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4883

0

4883

1653407

29

5080

338.6

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.367 ± 0.039

0.768 ± 0.01

5.957 ± 0.043

6.492 ± 0.035

5.078 ± 0.029

6.393 ± 0.051

1.854 ± 0.015

8.168 ± 0.036

6.98 ± 0.052

9.085 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.047 ± 0.019

5.815 ± 0.036

3.489 ± 0.024

3.655 ± 0.02

3.509 ± 0.027

6.508 ± 0.03

6.56 ± 0.047

6.065 ± 0.03

1.047 ± 0.013

4.164 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski