Sanxia sobemo-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEU3|A0A1L3KEU3_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 4 OX=1923383 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84SLAYY6 pKa = 10.23HH7 pKa = 5.35SVLRR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.39TRR15 pKa = 11.84TDD17 pKa = 3.57GPSGNAKK24 pKa = 8.43TAILNGVLNEE34 pKa = 4.08FMDD37 pKa = 4.65AQWEE41 pKa = 4.34IPTDD45 pKa = 3.96FMTYY49 pKa = 6.87EE50 pKa = 3.86HH51 pKa = 6.75YY52 pKa = 10.89EE53 pKa = 4.07RR54 pKa = 11.84VLSKK58 pKa = 10.23IDD60 pKa = 3.32WTSSPGYY67 pKa = 9.37PYY69 pKa = 9.95MRR71 pKa = 11.84RR72 pKa = 11.84CPTNKK77 pKa = 9.54EE78 pKa = 3.52FFRR81 pKa = 11.84AVDD84 pKa = 3.92GQPSLLRR91 pKa = 11.84KK92 pKa = 7.95NQVWEE97 pKa = 4.02MVQRR101 pKa = 11.84RR102 pKa = 11.84LRR104 pKa = 11.84EE105 pKa = 3.65RR106 pKa = 11.84DD107 pKa = 3.16ADD109 pKa = 4.38PIRR112 pKa = 11.84LFIKK116 pKa = 10.27PEE118 pKa = 3.6PHH120 pKa = 7.13KK121 pKa = 10.59LQKK124 pKa = 10.9LEE126 pKa = 4.18DD127 pKa = 3.32GRR129 pKa = 11.84YY130 pKa = 9.06RR131 pKa = 11.84LISSVSVIDD140 pKa = 4.14QIIDD144 pKa = 3.23HH145 pKa = 6.6MIFGEE150 pKa = 4.23MNEE153 pKa = 4.53KK154 pKa = 10.24LVQNWNYY161 pKa = 10.03VPPKK165 pKa = 10.23IGWSQYY171 pKa = 8.47NGGWRR176 pKa = 11.84SIPTEE181 pKa = 3.7RR182 pKa = 11.84QMALDD187 pKa = 4.12KK188 pKa = 11.19KK189 pKa = 9.49CWDD192 pKa = 3.28WTVQIWVMEE201 pKa = 4.86FILEE205 pKa = 3.93VRR207 pKa = 11.84MRR209 pKa = 11.84LCKK212 pKa = 10.52NLTDD216 pKa = 3.51EE217 pKa = 4.57WKK219 pKa = 10.74DD220 pKa = 3.36LATWRR225 pKa = 11.84YY226 pKa = 9.32KK227 pKa = 10.9SLFVDD232 pKa = 4.06PVFITSGGLMLRR244 pKa = 11.84QRR246 pKa = 11.84NPGVMKK252 pKa = 10.22SGCVLTIGDD261 pKa = 3.8NSIGQVLMHH270 pKa = 6.48HH271 pKa = 6.13RR272 pKa = 11.84VCFEE276 pKa = 3.68NDD278 pKa = 3.16IPPGTIMCMGDD289 pKa = 3.51DD290 pKa = 4.03TLQSVPEE297 pKa = 3.99NTRR300 pKa = 11.84AYY302 pKa = 9.75LDD304 pKa = 3.66TMASFCKK311 pKa = 10.6VKK313 pKa = 10.84DD314 pKa = 3.95PIYY317 pKa = 9.6QSEE320 pKa = 4.19FAGMRR325 pKa = 11.84FYY327 pKa = 10.71PGGRR331 pKa = 11.84VEE333 pKa = 4.35PLYY336 pKa = 10.56KK337 pKa = 10.36GKK339 pKa = 9.49HH340 pKa = 5.42AFTLLHH346 pKa = 6.52VDD348 pKa = 3.92PKK350 pKa = 10.88VLPSLADD357 pKa = 3.51SYY359 pKa = 12.1ALLYY363 pKa = 10.35HH364 pKa = 6.69RR365 pKa = 11.84SSYY368 pKa = 11.22RR369 pKa = 11.84DD370 pKa = 3.29MFEE373 pKa = 5.43DD374 pKa = 4.19LLLKK378 pKa = 10.1MGQKK382 pKa = 10.04VVDD385 pKa = 3.71RR386 pKa = 11.84EE387 pKa = 4.29SRR389 pKa = 11.84DD390 pKa = 3.45VIYY393 pKa = 10.9DD394 pKa = 3.76GFF396 pKa = 4.53

Molecular weight: 46.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEU3|A0A1L3KEU3_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 4 OX=1923383 PE=4 SV=1
MM1 pKa = 7.0MWLAITGVTVMTFGFWCFANVLASAGRR28 pKa = 11.84RR29 pKa = 11.84SVQRR33 pKa = 11.84MRR35 pKa = 11.84GIRR38 pKa = 11.84MEE40 pKa = 4.13SVRR43 pKa = 11.84EE44 pKa = 3.8GSRR47 pKa = 11.84FEE49 pKa = 4.0RR50 pKa = 11.84AEE52 pKa = 3.87IPDD55 pKa = 3.41NQVAVRR61 pKa = 11.84IPGTFTDD68 pKa = 3.31EE69 pKa = 4.55HH70 pKa = 7.85IGYY73 pKa = 9.58GVRR76 pKa = 11.84FADD79 pKa = 3.71YY80 pKa = 10.45LVVPRR85 pKa = 11.84HH86 pKa = 4.84VLEE89 pKa = 5.09LSVQGKK95 pKa = 9.1LRR97 pKa = 11.84EE98 pKa = 4.06NVLLVGKK105 pKa = 9.64NGKK108 pKa = 10.11LIVTINATQSRR119 pKa = 11.84GCSDD123 pKa = 3.8LVYY126 pKa = 11.01VNLSAKK132 pKa = 8.13TWSILGAPHH141 pKa = 7.02IKK143 pKa = 8.76WANEE147 pKa = 3.84SKK149 pKa = 10.54NVQVTCTGMPGQSNGRR165 pKa = 11.84IRR167 pKa = 11.84KK168 pKa = 6.91TRR170 pKa = 11.84QEE172 pKa = 3.6WMVSYY177 pKa = 10.42EE178 pKa = 3.9GSTLPGMSGAAYY190 pKa = 9.36MNEE193 pKa = 3.68NLVMGVHH200 pKa = 5.92QGATGAFNMGFSSEE214 pKa = 4.27LVTAEE219 pKa = 3.72MSLLLRR225 pKa = 11.84NEE227 pKa = 4.16DD228 pKa = 3.51TGDD231 pKa = 3.53TNVEE235 pKa = 4.2PKK237 pKa = 10.0QSRR240 pKa = 11.84YY241 pKa = 10.07LSAVQDD247 pKa = 3.4QSFGKK252 pKa = 10.13NFYY255 pKa = 11.0DD256 pKa = 5.55RR257 pKa = 11.84IAALDD262 pKa = 3.9RR263 pKa = 11.84LEE265 pKa = 4.35KK266 pKa = 10.61KK267 pKa = 10.67YY268 pKa = 10.99GAGSDD273 pKa = 3.52WNLDD277 pKa = 2.9GAANYY282 pKa = 8.2DD283 pKa = 3.41QQFDD287 pKa = 3.97FEE289 pKa = 4.67SRR291 pKa = 11.84PRR293 pKa = 11.84PGVSNSSRR301 pKa = 11.84PVRR304 pKa = 11.84VAVPQGGVQLVNHH317 pKa = 6.25NQTGRR322 pKa = 11.84TTLIEE327 pKa = 4.19VQHH330 pKa = 7.33QDD332 pKa = 3.22MLDD335 pKa = 3.17AFANARR341 pKa = 11.84VLDD344 pKa = 3.95RR345 pKa = 11.84LAVLEE350 pKa = 4.16RR351 pKa = 11.84AVGEE355 pKa = 4.41LRR357 pKa = 11.84AQQPPLEE364 pKa = 4.25NDD366 pKa = 3.15RR367 pKa = 11.84LVALEE372 pKa = 3.78QAVRR376 pKa = 11.84DD377 pKa = 3.89LKK379 pKa = 10.39PRR381 pKa = 11.84EE382 pKa = 4.05MPVVMEE388 pKa = 4.67RR389 pKa = 11.84AQPSYY394 pKa = 11.78AEE396 pKa = 4.23VAKK399 pKa = 9.18TAPSEE404 pKa = 3.93KK405 pKa = 10.17KK406 pKa = 9.35PRR408 pKa = 11.84KK409 pKa = 9.64KK410 pKa = 10.38YY411 pKa = 10.41ACKK414 pKa = 10.16LCNIVTRR421 pKa = 11.84TQMRR425 pKa = 11.84LDD427 pKa = 3.2NHH429 pKa = 6.3IASNHH434 pKa = 4.81KK435 pKa = 8.56TGQVQMEE442 pKa = 4.3SAIPSDD448 pKa = 3.37TGKK451 pKa = 10.16SGKK454 pKa = 8.97IIKK457 pKa = 9.32MGSFLEE463 pKa = 4.4KK464 pKa = 10.15RR465 pKa = 11.84SSSPRR470 pKa = 11.84NKK472 pKa = 10.06SNQSSRR478 pKa = 11.84SSRR481 pKa = 11.84FSEE484 pKa = 4.38KK485 pKa = 10.18KK486 pKa = 9.15NHH488 pKa = 6.11SPLPEE493 pKa = 4.73GSLSEE498 pKa = 4.41MIASQRR504 pKa = 11.84SISADD509 pKa = 3.2LKK511 pKa = 10.37EE512 pKa = 4.26LLKK515 pKa = 11.43VMVGQSSAMTQKK527 pKa = 10.62CGVSPTTQCC536 pKa = 4.35

Molecular weight: 59.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

932

396

536

466.0

52.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.901 ± 1.56

1.502 ± 0.175

5.365 ± 1.292

5.687 ± 0.253

3.326 ± 0.471

6.867 ± 0.532

1.931 ± 0.225

4.292 ± 0.667

5.472 ± 0.055

8.155 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.292 ± 0.334

4.506 ± 0.64

4.828 ± 0.48

4.828 ± 0.686

7.725 ± 0.068

7.94 ± 1.239

4.828 ± 0.187

7.725 ± 0.598

1.824 ± 0.629

3.004 ± 0.85
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski