Average proteome isoelectric point is 7.3

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

    

All

Note: above files contain also dissociation constants (pKa)

Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

    

>tr|U3RHX2|U3RHX2_9VIRU Envelopment polyprotein OS=Murrumbidgee virus OX=1406134 PE=4 SV=1
MM1 pKa = 7.16EE2 pKa = 4.56QNLNQLLARR11 pKa = 11.84INAAKK16 pKa = 9.78EE17 pKa = 3.53ATIARR22 pKa = 11.84DD23 pKa = 2.89IHH25 pKa = 6.13TDD27 pKa = 3.51LMQLRR32 pKa = 11.84HH33 pKa = 6.92DD34 pKa = 3.96YY35 pKa = 10.46FGQEE39 pKa = 3.48ICKK42 pKa = 10.58SLDD45 pKa = 2.79IEE47 pKa = 4.35YY48 pKa = 10.72RR49 pKa = 11.84NDD51 pKa = 3.18VPLEE55 pKa = 4.42KK56 pKa = 10.08ILDD59 pKa = 4.53EE60 pKa = 4.73ILDD63 pKa = 4.39DD64 pKa = 4.8NDD66 pKa = 3.94IEE68 pKa = 4.53NIPYY72 pKa = 8.19ITPDD76 pKa = 3.22NYY78 pKa = 11.47LLIDD82 pKa = 3.57NTLFIIDD89 pKa = 4.02YY90 pKa = 8.75KK91 pKa = 11.09VSVSMEE97 pKa = 4.09STTQTLKK104 pKa = 10.82KK105 pKa = 10.03YY106 pKa = 10.48AEE108 pKa = 4.16KK109 pKa = 11.1LEE111 pKa = 4.94LIEE114 pKa = 6.37DD115 pKa = 4.23YY116 pKa = 10.52IPYY119 pKa = 9.71KK120 pKa = 10.51IEE122 pKa = 4.07VVVLKK127 pKa = 10.16IDD129 pKa = 4.34PYY131 pKa = 10.5TKK133 pKa = 10.1QLYY136 pKa = 9.82ISSNTFANLFPNIHH150 pKa = 6.8LDD152 pKa = 3.58VNFEE156 pKa = 4.05PFIDD160 pKa = 4.03LRR162 pKa = 11.84NLLLEE167 pKa = 4.57KK168 pKa = 10.78FSDD171 pKa = 4.22DD172 pKa = 3.32EE173 pKa = 5.25DD174 pKa = 3.95FATMIAHH181 pKa = 7.57GDD183 pKa = 3.56FTMTSSWTDD192 pKa = 3.27EE193 pKa = 4.38SCKK196 pKa = 10.28EE197 pKa = 4.16LYY199 pKa = 9.87NHH201 pKa = 7.79PIYY204 pKa = 10.88KK205 pKa = 9.31EE206 pKa = 4.15FKK208 pKa = 7.73YY209 pKa = 10.97SMPIPYY215 pKa = 9.63RR216 pKa = 11.84RR217 pKa = 11.84LFEE220 pKa = 3.67EE221 pKa = 5.13AINHH225 pKa = 5.87NSYY228 pKa = 10.79KK229 pKa = 9.79SEE231 pKa = 3.69RR232 pKa = 11.84WNSFLIKK239 pKa = 10.29LRR241 pKa = 11.84EE242 pKa = 4.08YY243 pKa = 10.46TKK245 pKa = 10.89APYY248 pKa = 10.39SDD250 pKa = 5.37FIEE253 pKa = 4.54EE254 pKa = 4.02MSKK257 pKa = 10.52QIYY260 pKa = 8.13STDD263 pKa = 3.16GNYY266 pKa = 8.45PKK268 pKa = 9.59PDD270 pKa = 3.01KK271 pKa = 11.07SAILKK276 pKa = 10.11GWDD279 pKa = 3.39DD280 pKa = 3.55MRR282 pKa = 11.84DD283 pKa = 3.57RR284 pKa = 11.84IQGMRR289 pKa = 11.84TMSDD293 pKa = 2.92NVSDD297 pKa = 4.7AKK299 pKa = 10.78PSAHH303 pKa = 6.91FIWAEE308 pKa = 3.51HH309 pKa = 5.96DD310 pKa = 3.88TNRR313 pKa = 11.84SNHH316 pKa = 6.53NITKK320 pKa = 10.44LLYY323 pKa = 10.17LSKK326 pKa = 10.59SLQQIKK332 pKa = 8.55GTDD335 pKa = 3.91PIEE338 pKa = 4.12RR339 pKa = 11.84SYY341 pKa = 11.71AAIGKK346 pKa = 8.48MMDD349 pKa = 3.99FSSHH353 pKa = 5.95IEE355 pKa = 4.18EE356 pKa = 4.09YY357 pKa = 10.65TEE359 pKa = 5.78LIEE362 pKa = 4.61KK363 pKa = 10.1LKK365 pKa = 11.34NEE367 pKa = 3.67ARR369 pKa = 11.84LSKK372 pKa = 10.63RR373 pKa = 11.84KK374 pKa = 9.9ISDD377 pKa = 3.44TKK379 pKa = 11.02LKK381 pKa = 7.91PTPLGSAQVLWEE393 pKa = 3.9QQFLFNNDD401 pKa = 4.71LISKK405 pKa = 9.56DD406 pKa = 3.99DD407 pKa = 3.41KK408 pKa = 9.97RR409 pKa = 11.84HH410 pKa = 5.53FYY412 pKa = 10.68KK413 pKa = 10.5KK414 pKa = 9.14YY415 pKa = 10.45LGIGGHH421 pKa = 5.57KK422 pKa = 10.2QFTNKK427 pKa = 8.63TMSDD431 pKa = 3.37LDD433 pKa = 3.65INQPKK438 pKa = 10.14ILDD441 pKa = 3.99FDD443 pKa = 3.94NKK445 pKa = 10.78DD446 pKa = 3.14IILAARR452 pKa = 11.84SLINRR457 pKa = 11.84TKK459 pKa = 10.44RR460 pKa = 11.84HH461 pKa = 5.79LSSLSNLNYY470 pKa = 9.93SEE472 pKa = 4.62HH473 pKa = 7.39PIMDD477 pKa = 3.47YY478 pKa = 9.53MAEE481 pKa = 4.02IKK483 pKa = 10.32KK484 pKa = 10.9CSDD487 pKa = 3.4DD488 pKa = 3.35SSNIINQILATNFWACLNDD507 pKa = 3.09ISVIMKK513 pKa = 10.1NLLSVAQYY521 pKa = 11.54NKK523 pKa = 9.64INTFRR528 pKa = 11.84VVTCANNSVFGFLFPSSDD546 pKa = 2.89IKK548 pKa = 10.45SQRR551 pKa = 11.84STLLYY556 pKa = 10.48SIVIIHH562 pKa = 6.65KK563 pKa = 9.53MPLIFEE569 pKa = 4.52GSSLYY574 pKa = 9.41RR575 pKa = 11.84TIAFRR580 pKa = 11.84DD581 pKa = 3.87GFISISKK588 pKa = 10.42AIRR591 pKa = 11.84LDD593 pKa = 3.1KK594 pKa = 10.92ARR596 pKa = 11.84CQRR599 pKa = 11.84LVLSPAMFLQTSLLFKK615 pKa = 10.83GDD617 pKa = 3.69NQFLNLMDD625 pKa = 3.49ICTFAFFTSISITKK639 pKa = 10.49AMLSLTEE646 pKa = 3.87PARR649 pKa = 11.84YY650 pKa = 9.43MMMNALAVSSHH661 pKa = 4.0VRR663 pKa = 11.84EE664 pKa = 4.23YY665 pKa = 10.62IADD668 pKa = 3.67KK669 pKa = 10.43FSPYY673 pKa = 9.99TKK675 pKa = 9.71TLFSVYY681 pKa = 9.63VCNLIKK687 pKa = 10.71VGCLNANAQRR697 pKa = 11.84EE698 pKa = 4.4NIKK701 pKa = 10.88LRR703 pKa = 11.84DD704 pKa = 3.63VYY706 pKa = 11.45LNDD709 pKa = 3.4YY710 pKa = 10.56EE711 pKa = 5.08IIQKK715 pKa = 9.57GVKK718 pKa = 9.35NEE720 pKa = 3.95KK721 pKa = 10.33LLDD724 pKa = 4.49SIWFPGKK731 pKa = 10.24VDD733 pKa = 3.74LKK735 pKa = 11.17EE736 pKa = 5.01FITQIYY742 pKa = 9.9LPFYY746 pKa = 10.16FNAKK750 pKa = 9.18GLHH753 pKa = 5.86EE754 pKa = 4.17KK755 pKa = 10.32HH756 pKa = 6.2HH757 pKa = 5.99VLVNLAKK764 pKa = 9.86TIVEE768 pKa = 4.0IEE770 pKa = 4.0LEE772 pKa = 3.87QRR774 pKa = 11.84QIKK777 pKa = 9.6DD778 pKa = 2.78IWSSEE783 pKa = 4.0PKK785 pKa = 9.97KK786 pKa = 9.84QTVNLPVFVHH796 pKa = 7.12ALTKK800 pKa = 10.83NLILDD805 pKa = 4.11TAKK808 pKa = 10.66HH809 pKa = 4.26NHH811 pKa = 5.27LRR813 pKa = 11.84HH814 pKa = 5.67RR815 pKa = 11.84VEE817 pKa = 3.81SRR819 pKa = 11.84NNFKK823 pKa = 10.8RR824 pKa = 11.84SITTIKK830 pKa = 9.77TFTSSKK836 pKa = 9.91SCIEE840 pKa = 3.74IGDD843 pKa = 3.83FKK845 pKa = 11.41DD846 pKa = 3.94FKK848 pKa = 10.71TKK850 pKa = 10.28ISTKK854 pKa = 7.09QQKK857 pKa = 9.39LLKK860 pKa = 10.22KK861 pKa = 8.61EE862 pKa = 4.0VEE864 pKa = 4.31RR865 pKa = 11.84TRR867 pKa = 11.84IACPDD872 pKa = 3.49LVDD875 pKa = 5.9EE876 pKa = 5.16IDD878 pKa = 3.92HH879 pKa = 6.04NLKK882 pKa = 9.94IAHH885 pKa = 6.73ANYY888 pKa = 9.94NLLRR892 pKa = 11.84SSVPNYY898 pKa = 10.49VDD900 pKa = 4.7FISIKK905 pKa = 10.66VFDD908 pKa = 4.9ALYY911 pKa = 8.74KK912 pKa = 8.99TDD914 pKa = 4.25KK915 pKa = 10.96SVLEE919 pKa = 4.13QFPDD923 pKa = 3.92TISYY927 pKa = 11.03AMHH930 pKa = 6.31TMRR933 pKa = 11.84TKK935 pKa = 10.64KK936 pKa = 10.36DD937 pKa = 3.7FYY939 pKa = 10.37FTFFNKK945 pKa = 8.48EE946 pKa = 3.3QKK948 pKa = 9.55TYY950 pKa = 9.61VDD952 pKa = 3.46RR953 pKa = 11.84EE954 pKa = 4.07IFVGEE959 pKa = 4.56FEE961 pKa = 5.66AKK963 pKa = 9.67LCMYY967 pKa = 10.03VLEE970 pKa = 5.36RR971 pKa = 11.84IAKK974 pKa = 7.75EE975 pKa = 3.74RR976 pKa = 11.84CKK978 pKa = 10.78LNPEE982 pKa = 4.09EE983 pKa = 4.85MISEE987 pKa = 4.47PGDD990 pKa = 3.38NKK992 pKa = 10.93LKK994 pKa = 10.52ILEE997 pKa = 4.04QRR999 pKa = 11.84SEE1001 pKa = 3.72IEE1003 pKa = 3.5TRR1005 pKa = 11.84YY1006 pKa = 10.53LINQTLAHH1014 pKa = 6.37NKK1016 pKa = 10.12AILAKK1021 pKa = 9.55IDD1023 pKa = 3.76KK1024 pKa = 9.71TPLDD1028 pKa = 3.59EE1029 pKa = 4.35TALKK1033 pKa = 10.49RR1034 pKa = 11.84GFRR1037 pKa = 11.84LEE1039 pKa = 3.99INADD1043 pKa = 3.37MSKK1046 pKa = 10.21WSAQDD1051 pKa = 3.15VMYY1054 pKa = 11.06KK1055 pKa = 9.65FFWLFACDD1063 pKa = 4.15PILYY1067 pKa = 9.32PNEE1070 pKa = 4.29KK1071 pKa = 10.07EE1072 pKa = 3.95HH1073 pKa = 8.01ILYY1076 pKa = 9.23FLCNYY1081 pKa = 7.75MSKK1084 pKa = 10.6KK1085 pKa = 10.47VLLPDD1090 pKa = 4.01NLMCNLLDD1098 pKa = 3.62QKK1100 pKa = 11.09VIYY1103 pKa = 9.74EE1104 pKa = 3.96NDD1106 pKa = 3.22MLKK1109 pKa = 10.55EE1110 pKa = 3.7ITNGFRR1116 pKa = 11.84TNSIDD1121 pKa = 4.29VKK1123 pKa = 11.02RR1124 pKa = 11.84NWLQGNFNYY1133 pKa = 9.95VSSYY1137 pKa = 7.34MHH1139 pKa = 5.97TVMMASYY1146 pKa = 11.04KK1147 pKa = 10.5DD1148 pKa = 3.75VLKK1151 pKa = 9.62KK1152 pKa = 7.96THH1154 pKa = 6.53EE1155 pKa = 4.08NAEE1158 pKa = 4.26LVVNSLVHH1166 pKa = 7.08SDD1168 pKa = 4.37DD1169 pKa = 3.7NQTSIVLIQDD1179 pKa = 3.96KK1180 pKa = 10.44FPEE1183 pKa = 4.35EE1184 pKa = 4.19VAIHH1188 pKa = 6.14HH1189 pKa = 6.81LLNTFEE1195 pKa = 5.07KK1196 pKa = 9.9ICLTFGCQANMKK1208 pKa = 8.45KK1209 pKa = 8.82TYY1211 pKa = 8.24ATNFIKK1217 pKa = 10.69EE1218 pKa = 4.21FVSLFNICGEE1228 pKa = 4.14PYY1230 pKa = 10.14SIYY1233 pKa = 10.89GRR1235 pKa = 11.84FILTSVSDD1243 pKa = 3.9CAYY1246 pKa = 10.31LGPYY1250 pKa = 9.23EE1251 pKa = 5.1DD1252 pKa = 3.57LASRR1256 pKa = 11.84ISAAQTAIKK1265 pKa = 9.48HH1266 pKa = 5.41GCPASIAWLAIACAQWVTSLTYY1288 pKa = 11.41NMLPNQRR1295 pKa = 11.84NDD1297 pKa = 3.32PAKK1300 pKa = 10.75VLNCSKK1306 pKa = 10.53WEE1308 pKa = 3.83IPMEE1312 pKa = 4.27LGGNINAPLYY1322 pKa = 9.17LTALVGLEE1330 pKa = 3.66SDD1332 pKa = 3.82RR1333 pKa = 11.84LHH1335 pKa = 6.66FLLNILRR1342 pKa = 11.84KK1343 pKa = 9.19LVKK1346 pKa = 9.86INYY1349 pKa = 8.29IRR1351 pKa = 11.84EE1352 pKa = 4.2DD1353 pKa = 3.13VSVQVKK1359 pKa = 10.3NLDD1362 pKa = 3.36EE1363 pKa = 4.33LKK1365 pKa = 10.32IIKK1368 pKa = 10.21LSEE1371 pKa = 3.7IEE1373 pKa = 4.19LFKK1376 pKa = 11.25LKK1378 pKa = 10.63LIRR1381 pKa = 11.84YY1382 pKa = 8.97LSFDD1386 pKa = 3.84TNLSLNDD1393 pKa = 3.94TMGEE1397 pKa = 4.13TCDD1400 pKa = 3.39MKK1402 pKa = 10.92SRR1404 pKa = 11.84SILTPRR1410 pKa = 11.84KK1411 pKa = 8.05FTTSGILKK1419 pKa = 10.06KK1420 pKa = 10.28LASYY1424 pKa = 10.87RR1425 pKa = 11.84DD1426 pKa = 3.93YY1427 pKa = 11.6QHH1429 pKa = 6.31TTDD1432 pKa = 4.62NPLSLEE1438 pKa = 4.17SIYY1441 pKa = 10.82DD1442 pKa = 3.57YY1443 pKa = 11.04MLANPEE1449 pKa = 4.11LLVTKK1454 pKa = 10.76GEE1456 pKa = 4.27TFDD1459 pKa = 3.9QFKK1462 pKa = 10.96KK1463 pKa = 10.56CILYY1467 pKa = 10.14RR1468 pKa = 11.84YY1469 pKa = 8.07QSKK1472 pKa = 10.44KK1473 pKa = 10.0FRR1475 pKa = 11.84EE1476 pKa = 4.27SLSIQNPVQLFIEE1489 pKa = 4.51QILFSHH1495 pKa = 6.97KK1496 pKa = 9.35PTIDD1500 pKa = 3.31YY1501 pKa = 11.2ASIFEE1506 pKa = 4.56GTDD1509 pKa = 2.92ITPDD1513 pKa = 3.24SLIDD1517 pKa = 3.62EE1518 pKa = 4.54KK1519 pKa = 11.59HH1520 pKa = 6.26NIHH1523 pKa = 6.73GNLTISQTLQALKK1536 pKa = 10.64SDD1538 pKa = 4.22LSRR1541 pKa = 11.84LEE1543 pKa = 4.02LTLTDD1548 pKa = 4.16IKK1550 pKa = 11.16LCLTFTILNDD1560 pKa = 4.0PLMTMAANSYY1570 pKa = 10.83IMGITGDD1577 pKa = 3.33KK1578 pKa = 10.61SEE1580 pKa = 4.24RR1581 pKa = 11.84TGLSCCTMPEE1591 pKa = 4.0FRR1593 pKa = 11.84NMRR1596 pKa = 11.84VVHH1599 pKa = 6.43HH1600 pKa = 6.65SPALILRR1607 pKa = 11.84AYY1609 pKa = 9.7AHH1611 pKa = 6.58SNPDD1615 pKa = 3.15QVGADD1620 pKa = 3.69IIEE1623 pKa = 4.14MQRR1626 pKa = 11.84DD1627 pKa = 3.63LLHH1630 pKa = 6.22VEE1632 pKa = 3.98NFIKK1636 pKa = 9.47EE1637 pKa = 3.98THH1639 pKa = 5.23IQEE1642 pKa = 4.35RR1643 pKa = 11.84MLANMASKK1651 pKa = 10.39EE1652 pKa = 4.05FEE1654 pKa = 4.13GLDD1657 pKa = 3.3KK1658 pKa = 11.1KK1659 pKa = 10.89RR1660 pKa = 11.84FEE1662 pKa = 4.5LKK1664 pKa = 10.27EE1665 pKa = 3.7LTRR1668 pKa = 11.84FYY1670 pKa = 10.2QTIYY1674 pKa = 10.8NYY1676 pKa = 10.42VKK1678 pKa = 9.84STEE1681 pKa = 4.13HH1682 pKa = 5.96KK1683 pKa = 9.81VKK1685 pKa = 10.67IFILPHH1691 pKa = 5.59KK1692 pKa = 9.86AYY1694 pKa = 9.89TNIDD1698 pKa = 3.82FCSLLQGNLISDD1710 pKa = 3.66KK1711 pKa = 10.66TYY1713 pKa = 8.47TTIHH1717 pKa = 6.49YY1718 pKa = 8.93IKK1720 pKa = 10.52QIASYY1725 pKa = 8.76TKK1727 pKa = 9.06KK1728 pKa = 10.71AHH1730 pKa = 6.61IKK1732 pKa = 8.45TSPNIEE1738 pKa = 3.61INIAKK1743 pKa = 9.83EE1744 pKa = 3.79AFRR1747 pKa = 11.84VLSFFLDD1754 pKa = 3.53TFLSIDD1760 pKa = 3.31SRR1762 pKa = 11.84IPALRR1767 pKa = 11.84KK1768 pKa = 8.79MIEE1771 pKa = 3.82NFTYY1775 pKa = 8.76KK1776 pKa = 9.99TLPLKK1781 pKa = 10.84YY1782 pKa = 10.1LLQLIEE1788 pKa = 3.95NSQVRR1793 pKa = 11.84FEE1795 pKa = 4.61FLPLLYY1801 pKa = 10.27RR1802 pKa = 11.84LEE1804 pKa = 4.36KK1805 pKa = 10.91LEE1807 pKa = 4.29QSDD1810 pKa = 3.9LDD1812 pKa = 4.32RR1813 pKa = 11.84YY1814 pKa = 11.1DD1815 pKa = 3.61SLKK1818 pKa = 10.86SDD1820 pKa = 6.12DD1821 pKa = 4.57NITWNQWQVNRR1832 pKa = 11.84TFDD1835 pKa = 3.37KK1836 pKa = 11.65GEE1838 pKa = 3.71IDD1840 pKa = 5.9LILKK1844 pKa = 10.5GKK1846 pKa = 10.07DD1847 pKa = 3.1KK1848 pKa = 10.69YY1849 pKa = 10.93IHH1851 pKa = 7.04IIGIDD1856 pKa = 4.4DD1857 pKa = 3.48ILKK1860 pKa = 10.01IAEE1863 pKa = 4.01LHH1865 pKa = 6.53IPTKK1869 pKa = 10.42KK1870 pKa = 10.63NEE1872 pKa = 4.63DD1873 pKa = 2.92IRR1875 pKa = 11.84AVGRR1879 pKa = 11.84KK1880 pKa = 8.88LLNQNHH1886 pKa = 5.94GLRR1889 pKa = 11.84FEE1891 pKa = 4.28KK1892 pKa = 9.79MKK1894 pKa = 10.92SIITEE1899 pKa = 3.6PDD1901 pKa = 2.75NYY1903 pKa = 10.33YY1904 pKa = 9.95ICYY1907 pKa = 8.43QKK1909 pKa = 10.91RR1910 pKa = 11.84MRR1912 pKa = 11.84NQYY1915 pKa = 9.55HH1916 pKa = 5.79YY1917 pKa = 10.0CIQSTDD1923 pKa = 3.71YY1924 pKa = 11.21IDD1926 pKa = 4.02FKK1928 pKa = 11.45NNEE1931 pKa = 3.88IRR1933 pKa = 11.84SKK1935 pKa = 10.93LSRR1938 pKa = 11.84EE1939 pKa = 4.01QNLMVPVCIVFIAIDD1954 pKa = 3.55IQRR1957 pKa = 11.84SRR1959 pKa = 11.84VLLRR1963 pKa = 11.84SIDD1966 pKa = 3.68YY1967 pKa = 10.97LNNEE1971 pKa = 3.98NFEE1974 pKa = 4.21VSRR1977 pKa = 11.84MKK1979 pKa = 9.44ITNDD1983 pKa = 3.12EE1984 pKa = 3.96FCTIKK1989 pKa = 10.3RR1990 pKa = 11.84AQMSKK1995 pKa = 8.18MQFFDD2000 pKa = 3.96GPEE2003 pKa = 3.81LASTIISINGLMRR2016 pKa = 11.84SPEE2019 pKa = 4.18LMNLNYY2025 pKa = 7.92TTITKK2030 pKa = 10.64NSIISLAKK2038 pKa = 10.12IFQCSGNEE2046 pKa = 3.95KK2047 pKa = 10.73LDD2049 pKa = 3.69FQYY2052 pKa = 11.28LADD2055 pKa = 4.56EE2056 pKa = 4.39PMEE2059 pKa = 4.51DD2060 pKa = 4.48LEE2062 pKa = 6.22SITLDD2067 pKa = 3.8LEE2069 pKa = 4.21PALTIVHH2076 pKa = 6.29SKK2078 pKa = 9.61RR2079 pKa = 11.84ANRR2082 pKa = 11.84LQTFRR2087 pKa = 11.84NAITKK2092 pKa = 9.58IVSDD2096 pKa = 4.8GIMEE2100 pKa = 4.54IEE2102 pKa = 4.67DD2103 pKa = 4.16SLDD2106 pKa = 3.04ISQDD2110 pKa = 3.17GFFSATNIGLLEE2122 pKa = 4.66AIIAIIDD2129 pKa = 3.98SIEE2132 pKa = 4.0TNEE2135 pKa = 3.7WSTILKK2141 pKa = 10.33NSIHH2145 pKa = 6.71IVFKK2149 pKa = 10.75HH2150 pKa = 5.24YY2151 pKa = 10.97DD2152 pKa = 3.43RR2153 pKa = 11.84DD2154 pKa = 3.94AEE2156 pKa = 4.08FHH2158 pKa = 6.23LFEE2161 pKa = 4.9MPIQFLINGEE2171 pKa = 4.12PNYY2174 pKa = 10.26TLIKK2178 pKa = 10.35EE2179 pKa = 5.12FILSLPVLQNDD2190 pKa = 3.17TWSTIFEE2197 pKa = 4.32HH2198 pKa = 6.86FKK2200 pKa = 10.85QKK2202 pKa = 10.91ASAILDD2208 pKa = 3.58ALIMEE2213 pKa = 5.09KK2214 pKa = 10.08KK2215 pKa = 10.59DD2216 pKa = 3.13EE2217 pKa = 4.34GLNKK2221 pKa = 10.14FKK2223 pKa = 10.43TLLKK2227 pKa = 10.34KK2228 pKa = 10.15EE2229 pKa = 4.51SKK2231 pKa = 10.84GSFSGMFNFFSS2242 pKa = 3.68

Molecular weight: 260.58 kDa
Isoelectric point according different methods:

>tr|U3RF03|U3RF03_9VIRU Replicase OS=Murrumbidgee virus OX=1406134 PE=4 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.12IDD5 pKa = 4.69FVFDD9 pKa = 3.45DD10 pKa = 3.99TTSVVGDD17 pKa = 4.08FDD19 pKa = 4.06PKK21 pKa = 10.75AGYY24 pKa = 10.26EE25 pKa = 4.06YY26 pKa = 10.5FISNYY31 pKa = 8.36TSEE34 pKa = 5.07LNLGSLNTFFKK45 pKa = 11.05NVAKK49 pKa = 10.72AKK51 pKa = 10.1AQLRR55 pKa = 11.84MKK57 pKa = 10.58AKK59 pKa = 10.14RR60 pKa = 11.84QVTMKK65 pKa = 10.66FGDD68 pKa = 4.01LEE70 pKa = 4.53VVIHH74 pKa = 6.06NNNYY78 pKa = 9.68PGYY81 pKa = 9.69QNNEE85 pKa = 3.76LSTADD90 pKa = 3.64LTLHH94 pKa = 6.73RR95 pKa = 11.84LSAYY99 pKa = 9.67LIKK102 pKa = 10.85YY103 pKa = 9.14AADD106 pKa = 3.63EE107 pKa = 4.29VKK109 pKa = 10.68EE110 pKa = 3.92AMRR113 pKa = 11.84ANNRR117 pKa = 11.84AQAAVWKK124 pKa = 10.35GLILPLSLSHH134 pKa = 6.48GVTWEE139 pKa = 3.77HH140 pKa = 6.95GYY142 pKa = 10.1ILYY145 pKa = 9.74MSFTPGAEE153 pKa = 3.75MFMDD157 pKa = 3.8TFMFYY162 pKa = 10.32PVAIGIYY169 pKa = 9.39RR170 pKa = 11.84AKK172 pKa = 10.14QDD174 pKa = 3.84PEE176 pKa = 3.58QAQYY180 pKa = 11.01LKK182 pKa = 10.6KK183 pKa = 10.54ALRR186 pKa = 11.84QRR188 pKa = 11.84YY189 pKa = 8.27AGQKK193 pKa = 9.46AEE195 pKa = 3.78VWMVKK200 pKa = 9.02HH201 pKa = 5.79QKK203 pKa = 9.98DD204 pKa = 3.42VQAAVEE210 pKa = 4.34EE211 pKa = 4.64VSKK214 pKa = 11.13LPWTKK219 pKa = 11.01SSMSEE224 pKa = 3.68AARR227 pKa = 11.84QFLAKK232 pKa = 10.5FGIKK236 pKa = 9.22II237 pKa = 3.6

Molecular weight: 27.01 kDa
Isoelectric point according different methods:

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

For dipeptide frequency statistics click here

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here