Mesorhizobium denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371X631|A0A371X631_9RHIZ LysR family transcriptional regulator OS=Mesorhizobium denitrificans OX=2294114 GN=DY251_18920 PE=3 SV=1
MM1 pKa = 7.72VGGTLLALALYY12 pKa = 8.16AAPASAQSAGSGCGIQQAADD32 pKa = 3.32LAMQRR37 pKa = 11.84QIALLDD43 pKa = 3.71AAKK46 pKa = 10.39VDD48 pKa = 3.49SSKK51 pKa = 10.76FFNGANSCLGANLLKK66 pKa = 11.04SFDD69 pKa = 4.24LSNLIPSSMDD79 pKa = 3.08FLGSIGSSLIDD90 pKa = 3.82NVIQQATQQVCQVLNNQLQDD110 pKa = 3.04IVGKK114 pKa = 10.73INGQMFDD121 pKa = 4.0FNSLMGSQMSDD132 pKa = 2.96LLGGSGSSISPINIPNLPNMGQYY155 pKa = 9.45TFTSASVPTSDD166 pKa = 5.11NPTSPIYY173 pKa = 10.09QPPAGNSGVNGSQTGTPAGSTTPDD197 pKa = 3.25LFNNMFSPAGQNGGG211 pKa = 3.53

Molecular weight: 21.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371XK65|A0A371XK65_9RHIZ GntR family transcriptional regulator OS=Mesorhizobium denitrificans OX=2294114 GN=DY251_01470 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.28SGRR28 pKa = 11.84QVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.99

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4119

0

4119

1302846

28

2855

316.3

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.881 ± 0.045

0.818 ± 0.012

5.681 ± 0.034

5.868 ± 0.034

3.871 ± 0.026

8.361 ± 0.038

1.988 ± 0.018

5.653 ± 0.027

3.909 ± 0.034

9.729 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.018

2.93 ± 0.024

4.92 ± 0.028

3.138 ± 0.019

6.705 ± 0.037

5.685 ± 0.027

5.298 ± 0.04

7.359 ± 0.034

1.298 ± 0.015

2.332 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski