Synergistes sp. 3_1_syn1

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Synergistes; unclassified Synergistes

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2957 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9PWS1|G9PWS1_9BACT Uncharacterized protein OS=Synergistes sp. 3_1_syn1 OX=457415 GN=HMPREF1006_00083 PE=4 SV=1
MM1 pKa = 7.58GIKK4 pKa = 10.38VNLDD8 pKa = 3.26DD9 pKa = 6.49CIGCGVCFEE18 pKa = 5.2LCPQNFKK25 pKa = 10.91LDD27 pKa = 3.85EE28 pKa = 4.72DD29 pKa = 3.87EE30 pKa = 4.61GKK32 pKa = 11.14CMVISNEE39 pKa = 4.08VNSSAKK45 pKa = 9.65EE46 pKa = 3.83AADD49 pKa = 3.68SCPVSAITIDD59 pKa = 3.17

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9PUR7|G9PUR7_9BACT Sodium ion-translocating decarboxylase beta subunit OS=Synergistes sp. 3_1_syn1 OX=457415 GN=HMPREF1006_02565 PE=4 SV=1
EEE2 pKa = 4.76EEE4 pKa = 4.02EEE6 pKa = 4.23IQGRR10 pKa = 11.84KKK12 pKa = 9.33IYYY15 pKa = 9.29CLYYY19 pKa = 9.91DD20 pKa = 4.24IVIKKK25 pKa = 10.37RR26 pKa = 11.84KKK28 pKa = 8.03

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2957

0

2957

957959

25

3571

324.0

35.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.142 ± 0.052

1.531 ± 0.019

5.198 ± 0.038

6.883 ± 0.047

4.115 ± 0.033

8.329 ± 0.048

1.604 ± 0.017

6.498 ± 0.036

5.564 ± 0.04

9.47 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.952 ± 0.021

3.36 ± 0.025

4.179 ± 0.023

2.349 ± 0.025

5.736 ± 0.042

5.878 ± 0.035

4.976 ± 0.041

6.968 ± 0.037

1.115 ± 0.018

3.156 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski