Amino acid dipepetide frequency for Thiomicrospira aerophila AL3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.801AlaAla: 8.801 ± 0.161
0.965AlaCys: 0.965 ± 0.039
5.412AlaAsp: 5.412 ± 0.092
6.112AlaGlu: 6.112 ± 0.096
3.572AlaPhe: 3.572 ± 0.075
6.58AlaGly: 6.58 ± 0.111
2.077AlaHis: 2.077 ± 0.054
6.035AlaIle: 6.035 ± 0.108
5.174AlaLys: 5.174 ± 0.099
11.082AlaLeu: 11.082 ± 0.146
3.001AlaMet: 3.001 ± 0.071
3.61AlaAsn: 3.61 ± 0.075
3.292AlaPro: 3.292 ± 0.08
6.228AlaGln: 6.228 ± 0.136
4.657AlaArg: 4.657 ± 0.099
5.316AlaSer: 5.316 ± 0.105
4.615AlaThr: 4.615 ± 0.086
6.021AlaVal: 6.021 ± 0.114
1.861AlaTrp: 1.861 ± 0.066
2.591AlaTyr: 2.591 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.036
0.107CysCys: 0.107 ± 0.013
0.477CysAsp: 0.477 ± 0.025
0.489CysGlu: 0.489 ± 0.027
0.304CysPhe: 0.304 ± 0.02
0.657CysGly: 0.657 ± 0.04
0.31CysHis: 0.31 ± 0.02
0.462CysIle: 0.462 ± 0.027
0.261CysLys: 0.261 ± 0.019
0.897CysLeu: 0.897 ± 0.04
0.162CysMet: 0.162 ± 0.016
0.284CysAsn: 0.284 ± 0.019
0.453CysPro: 0.453 ± 0.029
0.52CysGln: 0.52 ± 0.027
0.409CysArg: 0.409 ± 0.029
0.456CysSer: 0.456 ± 0.029
0.295CysThr: 0.295 ± 0.02
0.536CysVal: 0.536 ± 0.029
0.103CysTrp: 0.103 ± 0.014
0.281CysTyr: 0.281 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
5.06AspAla: 5.06 ± 0.089
0.447AspCys: 0.447 ± 0.027
3.018AspAsp: 3.018 ± 0.081
3.628AspGlu: 3.628 ± 0.067
2.604AspPhe: 2.604 ± 0.065
3.363AspGly: 3.363 ± 0.082
1.264AspHis: 1.264 ± 0.052
3.577AspIle: 3.577 ± 0.079
2.437AspLys: 2.437 ± 0.065
6.196AspLeu: 6.196 ± 0.12
1.431AspMet: 1.431 ± 0.043
1.905AspAsn: 1.905 ± 0.06
2.426AspPro: 2.426 ± 0.059
3.27AspGln: 3.27 ± 0.069
2.648AspArg: 2.648 ± 0.067
2.687AspSer: 2.687 ± 0.072
2.588AspThr: 2.588 ± 0.066
3.624AspVal: 3.624 ± 0.076
1.089AspTrp: 1.089 ± 0.049
2.382AspTyr: 2.382 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
5.375GluAla: 5.375 ± 0.097
0.376GluCys: 0.376 ± 0.024
2.562GluAsp: 2.562 ± 0.065
2.752GluGlu: 2.752 ± 0.079
2.36GluPhe: 2.36 ± 0.058
3.366GluGly: 3.366 ± 0.079
1.406GluHis: 1.406 ± 0.044
3.393GluIle: 3.393 ± 0.082
2.619GluLys: 2.619 ± 0.071
6.659GluLeu: 6.659 ± 0.115
1.562GluMet: 1.562 ± 0.052
1.888GluAsn: 1.888 ± 0.051
2.246GluPro: 2.246 ± 0.079
4.825GluGln: 4.825 ± 0.102
3.568GluArg: 3.568 ± 0.082
2.915GluSer: 2.915 ± 0.068
2.863GluThr: 2.863 ± 0.068
4.252GluVal: 4.252 ± 0.09
0.739GluTrp: 0.739 ± 0.037
1.381GluTyr: 1.381 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.612PheAla: 3.612 ± 0.075
0.402PheCys: 0.402 ± 0.025
2.965PheAsp: 2.965 ± 0.071
2.873PheGlu: 2.873 ± 0.063
1.631PhePhe: 1.631 ± 0.064
2.941PheGly: 2.941 ± 0.076
0.778PheHis: 0.778 ± 0.037
2.535PheIle: 2.535 ± 0.067
2.156PheLys: 2.156 ± 0.058
3.338PheLeu: 3.338 ± 0.089
1.079PheMet: 1.079 ± 0.04
2.204PheAsn: 2.204 ± 0.067
1.293PhePro: 1.293 ± 0.041
1.367PheGln: 1.367 ± 0.045
1.479PheArg: 1.479 ± 0.051
2.776PheSer: 2.776 ± 0.08
1.89PheThr: 1.89 ± 0.054
2.619PheVal: 2.619 ± 0.062
0.597PheTrp: 0.597 ± 0.032
1.4PheTyr: 1.4 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
5.072GlyAla: 5.072 ± 0.112
0.739GlyCys: 0.739 ± 0.036
3.553GlyAsp: 3.553 ± 0.082
3.986GlyGlu: 3.986 ± 0.083
3.174GlyPhe: 3.174 ± 0.081
4.509GlyGly: 4.509 ± 0.133
1.73GlyHis: 1.73 ± 0.056
3.955GlyIle: 3.955 ± 0.083
3.011GlyLys: 3.011 ± 0.074
8.763GlyLeu: 8.763 ± 0.149
1.941GlyMet: 1.941 ± 0.059
1.968GlyAsn: 1.968 ± 0.08
1.752GlyPro: 1.752 ± 0.053
3.763GlyGln: 3.763 ± 0.081
3.663GlyArg: 3.663 ± 0.082
3.35GlySer: 3.35 ± 0.091
2.938GlyThr: 2.938 ± 0.08
5.131GlyVal: 5.131 ± 0.079
1.11GlyTrp: 1.11 ± 0.048
2.396GlyTyr: 2.396 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
2.115HisAla: 2.115 ± 0.053
0.249HisCys: 0.249 ± 0.021
1.192HisAsp: 1.192 ± 0.043
1.151HisGlu: 1.151 ± 0.042
1.113HisPhe: 1.113 ± 0.037
1.589HisGly: 1.589 ± 0.056
0.869HisHis: 0.869 ± 0.041
1.343HisIle: 1.343 ± 0.041
0.911HisLys: 0.911 ± 0.035
2.746HisLeu: 2.746 ± 0.061
0.465HisMet: 0.465 ± 0.023
0.914HisAsn: 0.914 ± 0.038
1.556HisPro: 1.556 ± 0.053
1.819HisGln: 1.819 ± 0.06
1.174HisArg: 1.174 ± 0.04
1.276HisSer: 1.276 ± 0.048
1.119HisThr: 1.119 ± 0.04
1.073HisVal: 1.073 ± 0.043
0.464HisTrp: 0.464 ± 0.028
1.035HisTyr: 1.035 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.379IleAla: 6.379 ± 0.113
0.495IleCys: 0.495 ± 0.024
4.044IleAsp: 4.044 ± 0.082
4.538IleGlu: 4.538 ± 0.094
1.949IlePhe: 1.949 ± 0.048
4.264IleGly: 4.264 ± 0.096
1.396IleHis: 1.396 ± 0.047
3.441IleIle: 3.441 ± 0.079
3.307IleLys: 3.307 ± 0.066
5.311IleLeu: 5.311 ± 0.106
1.453IleMet: 1.453 ± 0.054
2.953IleAsn: 2.953 ± 0.081
2.325IlePro: 2.325 ± 0.06
2.653IleGln: 2.653 ± 0.065
2.846IleArg: 2.846 ± 0.066
3.538IleSer: 3.538 ± 0.078
3.489IleThr: 3.489 ± 0.078
3.711IleVal: 3.711 ± 0.087
0.696IleTrp: 0.696 ± 0.034
1.505IleTyr: 1.505 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
4.331LysAla: 4.331 ± 0.092
0.231LysCys: 0.231 ± 0.019
2.221LysAsp: 2.221 ± 0.059
2.095LysGlu: 2.095 ± 0.067
1.467LysPhe: 1.467 ± 0.043
2.734LysGly: 2.734 ± 0.065
1.148LysHis: 1.148 ± 0.042
2.782LysIle: 2.782 ± 0.07
2.295LysLys: 2.295 ± 0.064
5.02LysLeu: 5.02 ± 0.083
1.313LysMet: 1.313 ± 0.049
1.751LysAsn: 1.751 ± 0.056
2.295LysPro: 2.295 ± 0.056
2.958LysGln: 2.958 ± 0.072
2.556LysArg: 2.556 ± 0.07
2.506LysSer: 2.506 ± 0.069
2.855LysThr: 2.855 ± 0.061
3.246LysVal: 3.246 ± 0.072
0.497LysTrp: 0.497 ± 0.028
1.032LysTyr: 1.032 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
12.932LeuAla: 12.932 ± 0.204
0.781LeuCys: 0.781 ± 0.037
6.592LeuAsp: 6.592 ± 0.108
5.861LeuGlu: 5.861 ± 0.11
4.066LeuPhe: 4.066 ± 0.099
7.415LeuGly: 7.415 ± 0.137
2.287LeuHis: 2.287 ± 0.067
6.843LeuIle: 6.843 ± 0.118
5.168LeuLys: 5.168 ± 0.087
11.001LeuLeu: 11.001 ± 0.189
2.861LeuMet: 2.861 ± 0.078
5.062LeuAsn: 5.062 ± 0.112
5.035LeuPro: 5.035 ± 0.106
4.881LeuGln: 4.881 ± 0.092
5.091LeuArg: 5.091 ± 0.092
6.992LeuSer: 6.992 ± 0.098
6.326LeuThr: 6.326 ± 0.114
8.162LeuVal: 8.162 ± 0.154
1.266LeuTrp: 1.266 ± 0.056
2.485LeuTyr: 2.485 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.949MetAla: 2.949 ± 0.07
0.122MetCys: 0.122 ± 0.015
1.301MetAsp: 1.301 ± 0.045
1.026MetGlu: 1.026 ± 0.038
0.834MetPhe: 0.834 ± 0.038
1.796MetGly: 1.796 ± 0.05
0.576MetHis: 0.576 ± 0.029
1.628MetIle: 1.628 ± 0.052
1.222MetLys: 1.222 ± 0.044
2.699MetLeu: 2.699 ± 0.072
0.796MetMet: 0.796 ± 0.036
1.178MetAsn: 1.178 ± 0.045
1.429MetPro: 1.429 ± 0.051
1.427MetGln: 1.427 ± 0.053
1.361MetArg: 1.361 ± 0.043
1.837MetSer: 1.837 ± 0.062
1.728MetThr: 1.728 ± 0.049
1.714MetVal: 1.714 ± 0.059
0.205MetTrp: 0.205 ± 0.017
0.423MetTyr: 0.423 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.44AsnAla: 3.44 ± 0.081
0.264AsnCys: 0.264 ± 0.019
2.039AsnAsp: 2.039 ± 0.062
2.242AsnGlu: 2.242 ± 0.062
1.458AsnPhe: 1.458 ± 0.049
2.495AsnGly: 2.495 ± 0.08
0.97AsnHis: 0.97 ± 0.041
2.434AsnIle: 2.434 ± 0.08
1.702AsnLys: 1.702 ± 0.051
4.415AsnLeu: 4.415 ± 0.079
0.94AsnMet: 0.94 ± 0.04
1.739AsnAsn: 1.739 ± 0.064
2.418AsnPro: 2.418 ± 0.065
2.718AsnGln: 2.718 ± 0.078
2.128AsnArg: 2.128 ± 0.054
2.059AsnSer: 2.059 ± 0.074
1.967AsnThr: 1.967 ± 0.058
2.076AsnVal: 2.076 ± 0.065
0.687AsnTrp: 0.687 ± 0.03
1.137AsnTyr: 1.137 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
4.008ProAla: 4.008 ± 0.091
0.308ProCys: 0.308 ± 0.022
2.665ProAsp: 2.665 ± 0.069
2.825ProGlu: 2.825 ± 0.07
1.814ProPhe: 1.814 ± 0.058
2.968ProGly: 2.968 ± 0.064
1.005ProHis: 1.005 ± 0.036
2.693ProIle: 2.693 ± 0.07
1.997ProLys: 1.997 ± 0.058
4.388ProLeu: 4.388 ± 0.097
1.088ProMet: 1.088 ± 0.043
2.009ProAsn: 2.009 ± 0.057
1.375ProPro: 1.375 ± 0.045
1.938ProGln: 1.938 ± 0.054
1.642ProArg: 1.642 ± 0.053
2.514ProSer: 2.514 ± 0.066
2.174ProThr: 2.174 ± 0.062
3.254ProVal: 3.254 ± 0.077
0.55ProTrp: 0.55 ± 0.031
1.248ProTyr: 1.248 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
8.181GlnAla: 8.181 ± 0.172
0.372GlnCys: 0.372 ± 0.025
2.722GlnAsp: 2.722 ± 0.062
2.211GlnGlu: 2.211 ± 0.062
1.952GlnPhe: 1.952 ± 0.053
3.767GlnGly: 3.767 ± 0.085
1.595GlnHis: 1.595 ± 0.055
3.027GlnIle: 3.027 ± 0.076
2.143GlnLys: 2.143 ± 0.059
6.823GlnLeu: 6.823 ± 0.141
1.243GlnMet: 1.243 ± 0.046
1.85GlnAsn: 1.85 ± 0.063
2.773GlnPro: 2.773 ± 0.077
5.116GlnGln: 5.116 ± 0.153
3.198GlnArg: 3.198 ± 0.079
2.866GlnSer: 2.866 ± 0.079
3.187GlnThr: 3.187 ± 0.077
4.225GlnVal: 4.225 ± 0.076
0.695GlnTrp: 0.695 ± 0.034
1.147GlnTyr: 1.147 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
4.091ArgAla: 4.091 ± 0.082
0.399ArgCys: 0.399 ± 0.026
2.846ArgAsp: 2.846 ± 0.068
2.952ArgGlu: 2.952 ± 0.067
2.514ArgPhe: 2.514 ± 0.065
2.852ArgGly: 2.852 ± 0.07
1.341ArgHis: 1.341 ± 0.046
3.346ArgIle: 3.346 ± 0.073
1.959ArgLys: 1.959 ± 0.06
6.287ArgLeu: 6.287 ± 0.108
1.276ArgMet: 1.276 ± 0.051
1.89ArgAsn: 1.89 ± 0.049
2.062ArgPro: 2.062 ± 0.056
3.015ArgGln: 3.015 ± 0.084
2.479ArgArg: 2.479 ± 0.072
2.462ArgSer: 2.462 ± 0.056
2.036ArgThr: 2.036 ± 0.059
3.705ArgVal: 3.705 ± 0.083
0.763ArgTrp: 0.763 ± 0.035
1.853ArgTyr: 1.853 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
5.219SerAla: 5.219 ± 0.078
0.491SerCys: 0.491 ± 0.031
3.011SerAsp: 3.011 ± 0.068
3.104SerGlu: 3.104 ± 0.069
2.459SerPhe: 2.459 ± 0.064
4.143SerGly: 4.143 ± 0.094
1.53SerHis: 1.53 ± 0.044
3.168SerIle: 3.168 ± 0.074
2.402SerLys: 2.402 ± 0.057
6.645SerLeu: 6.645 ± 0.124
1.47SerMet: 1.47 ± 0.046
2.177SerAsn: 2.177 ± 0.076
2.264SerPro: 2.264 ± 0.057
3.134SerGln: 3.134 ± 0.078
2.825SerArg: 2.825 ± 0.06
3.304SerSer: 3.304 ± 0.091
2.474SerThr: 2.474 ± 0.069
3.941SerVal: 3.941 ± 0.072
0.856SerTrp: 0.856 ± 0.041
1.672SerTyr: 1.672 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
4.45ThrAla: 4.45 ± 0.092
0.344ThrCys: 0.344 ± 0.025
2.956ThrAsp: 2.956 ± 0.075
3.047ThrGlu: 3.047 ± 0.074
1.858ThrPhe: 1.858 ± 0.062
3.941ThrGly: 3.941 ± 0.109
1.417ThrHis: 1.417 ± 0.043
2.866ThrIle: 2.866 ± 0.071
1.974ThrLys: 1.974 ± 0.052
6.449ThrLeu: 6.449 ± 0.11
1.023ThrMet: 1.023 ± 0.042
1.752ThrAsn: 1.752 ± 0.064
2.979ThrPro: 2.979 ± 0.072
3.069ThrGln: 3.069 ± 0.073
2.512ThrArg: 2.512 ± 0.064
2.722ThrSer: 2.722 ± 0.064
2.636ThrThr: 2.636 ± 0.074
2.866ThrVal: 2.866 ± 0.068
0.592ThrTrp: 0.592 ± 0.033
1.278ThrTyr: 1.278 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
6.748ValAla: 6.748 ± 0.101
0.622ValCys: 0.622 ± 0.029
3.826ValAsp: 3.826 ± 0.073
4.047ValGlu: 4.047 ± 0.093
2.804ValPhe: 2.804 ± 0.069
4.47ValGly: 4.47 ± 0.101
1.289ValHis: 1.289 ± 0.044
4.609ValIle: 4.609 ± 0.087
3.221ValLys: 3.221 ± 0.07
6.781ValLeu: 6.781 ± 0.125
2.208ValMet: 2.208 ± 0.053
2.858ValAsn: 2.858 ± 0.069
2.441ValPro: 2.441 ± 0.061
2.69ValGln: 2.69 ± 0.071
3.355ValArg: 3.355 ± 0.074
4.396ValSer: 4.396 ± 0.091
3.82ValThr: 3.82 ± 0.09
4.991ValVal: 4.991 ± 0.104
0.856ValTrp: 0.856 ± 0.04
1.843ValTyr: 1.843 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.946TrpAla: 0.946 ± 0.045
0.137TrpCys: 0.137 ± 0.014
0.571TrpAsp: 0.571 ± 0.03
0.471TrpGlu: 0.471 ± 0.028
0.669TrpPhe: 0.669 ± 0.037
0.716TrpGly: 0.716 ± 0.03
0.453TrpHis: 0.453 ± 0.027
0.711TrpIle: 0.711 ± 0.03
0.32TrpLys: 0.32 ± 0.026
2.473TrpLeu: 2.473 ± 0.079
0.376TrpMet: 0.376 ± 0.027
0.367TrpAsn: 0.367 ± 0.024
0.696TrpPro: 0.696 ± 0.034
1.524TrpGln: 1.524 ± 0.061
0.879TrpArg: 0.879 ± 0.041
0.802TrpSer: 0.802 ± 0.038
0.536TrpThr: 0.536 ± 0.033
1.088TrpVal: 1.088 ± 0.047
0.24TrpTrp: 0.24 ± 0.022
0.337TrpTyr: 0.337 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.619TyrAla: 2.619 ± 0.067
0.285TyrCys: 0.285 ± 0.019
1.672TyrAsp: 1.672 ± 0.053
1.695TyrGlu: 1.695 ± 0.052
1.334TyrPhe: 1.334 ± 0.052
1.937TyrGly: 1.937 ± 0.058
0.733TyrHis: 0.733 ± 0.038
1.447TyrIle: 1.447 ± 0.045
1.053TyrLys: 1.053 ± 0.043
3.133TyrLeu: 3.133 ± 0.077
0.563TyrMet: 0.563 ± 0.03
0.968TyrAsn: 0.968 ± 0.04
1.35TyrPro: 1.35 ± 0.043
2.18TyrGln: 2.18 ± 0.07
1.659TyrArg: 1.659 ± 0.048
1.515TyrSer: 1.515 ± 0.053
1.195TyrThr: 1.195 ± 0.048
1.639TyrVal: 1.639 ± 0.048
0.447TyrTrp: 0.447 ± 0.025
0.873TyrTyr: 0.873 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2067 proteins (662005 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski