Staphylococcus phage phiSa119

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; unclassified Biseptimavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068A237|A0A068A237_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa119 OX=1498220 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.45VEE20 pKa = 3.82YY21 pKa = 10.92QHH23 pKa = 6.88FDD25 pKa = 3.86DD26 pKa = 6.2VDD28 pKa = 3.79DD29 pKa = 5.21EE30 pKa = 4.86KK31 pKa = 11.62DD32 pKa = 3.23ALADD36 pKa = 3.57YY37 pKa = 10.57LYY39 pKa = 10.94NNPDD43 pKa = 3.67EE44 pKa = 4.3ILEE47 pKa = 4.18YY48 pKa = 10.91DD49 pKa = 4.16SITIRR54 pKa = 11.84HH55 pKa = 6.25AYY57 pKa = 9.41IEE59 pKa = 4.35VEE61 pKa = 4.03

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068A203|A0A068A203_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa119 OX=1498220 PE=4 SV=1
MM1 pKa = 7.2VALLKK6 pKa = 10.65SLEE9 pKa = 3.97RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LMITISTMLQFGLFLIALIGLVIKK36 pKa = 10.76LIEE39 pKa = 4.32LSNKK43 pKa = 9.86KK44 pKa = 8.23ITIANFGWFRR54 pKa = 11.84WLNGYY59 pKa = 9.32

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13078

46

1514

192.3

22.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.353 ± 0.439

0.413 ± 0.092

6.155 ± 0.319

7.861 ± 0.488

4.03 ± 0.214

6.025 ± 0.556

1.827 ± 0.161

7.822 ± 0.256

9.933 ± 0.343

7.708 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.165

6.821 ± 0.23

2.638 ± 0.165

3.686 ± 0.177

4.114 ± 0.218

5.85 ± 0.313

5.643 ± 0.25

5.941 ± 0.246

1.193 ± 0.147

4.588 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski