Hubei sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEZ9|A0A1L3KEZ9_9VIRU Peptidase S39 domain-containing protein OS=Hubei sobemo-like virus 2 OX=1923205 PE=4 SV=1
MM1 pKa = 7.16MWTATLEE8 pKa = 4.01NSAIASRR15 pKa = 11.84QLQLSAVEE23 pKa = 3.95LWEE26 pKa = 4.98DD27 pKa = 3.41YY28 pKa = 11.17SYY30 pKa = 11.7LLNYY34 pKa = 9.55FFLLGVASVLAVVAIRR50 pKa = 11.84AAVNYY55 pKa = 10.07YY56 pKa = 10.61EE57 pKa = 4.31EE58 pKa = 4.98LRR60 pKa = 11.84LDD62 pKa = 3.61SLVRR66 pKa = 11.84EE67 pKa = 4.57LPTPPEE73 pKa = 4.25DD74 pKa = 3.53PVLDD78 pKa = 3.76GHH80 pKa = 7.62LEE82 pKa = 3.98FCPNNGYY89 pKa = 8.99VMRR92 pKa = 11.84VTTVIDD98 pKa = 4.0DD99 pKa = 3.99EE100 pKa = 4.42QHH102 pKa = 5.79SFLVKK107 pKa = 8.97VTPSQVTDD115 pKa = 3.47MLQGSSKK122 pKa = 8.06STRR125 pKa = 11.84VKK127 pKa = 10.05EE128 pKa = 4.07ARR130 pKa = 11.84GSSGVVPPGPRR141 pKa = 11.84AWDD144 pKa = 3.76AGNSTVPPAVHH155 pKa = 6.72LNGIPSAEE163 pKa = 3.87EE164 pKa = 3.43HH165 pKa = 7.18DD166 pKa = 3.66FDD168 pKa = 5.52LRR170 pKa = 11.84VVRR173 pKa = 11.84ALVRR177 pKa = 11.84MGVEE181 pKa = 3.7TRR183 pKa = 11.84EE184 pKa = 4.09NIQALGWDD192 pKa = 4.21FPEE195 pKa = 4.56ITASDD200 pKa = 4.03PQTPTTEE207 pKa = 4.04RR208 pKa = 11.84LLSEE212 pKa = 4.11TATSQEE218 pKa = 4.24LEE220 pKa = 4.11MAQPSSVVVPAQEE233 pKa = 4.46AFTKK237 pKa = 10.38KK238 pKa = 9.76ILKK241 pKa = 8.47ITDD244 pKa = 3.17RR245 pKa = 11.84EE246 pKa = 4.51GNVRR250 pKa = 11.84GLGFRR255 pKa = 11.84WEE257 pKa = 4.37NYY259 pKa = 9.72LVTAYY264 pKa = 10.05HH265 pKa = 6.34VYY267 pKa = 10.58EE268 pKa = 4.11SVKK271 pKa = 10.9NEE273 pKa = 3.92EE274 pKa = 4.73CDD276 pKa = 3.63VSPVPSTQGFPLSQFSALTYY296 pKa = 10.4SPKK299 pKa = 10.58LDD301 pKa = 4.29FIVLKK306 pKa = 10.37VDD308 pKa = 3.12KK309 pKa = 10.83PEE311 pKa = 4.08MVWAKK316 pKa = 10.39IGVSKK321 pKa = 10.64MSLKK325 pKa = 9.83TPAPNTPFKK334 pKa = 11.33VIGKK338 pKa = 8.9QNNSWNSSTGFLRR351 pKa = 11.84AAHH354 pKa = 6.79GDD356 pKa = 3.58LKK358 pKa = 10.57IKK360 pKa = 10.78HH361 pKa = 6.2NATTTKK367 pKa = 10.1GYY369 pKa = 10.2SGSPLLSDD377 pKa = 3.66GKK379 pKa = 11.15VVGLHH384 pKa = 6.62LGAANPTNYY393 pKa = 10.37CLDD396 pKa = 3.73LSFLPLLFQEE406 pKa = 5.05GVEE409 pKa = 4.24SEE411 pKa = 4.38EE412 pKa = 4.01KK413 pKa = 9.51QGRR416 pKa = 11.84YY417 pKa = 9.21HH418 pKa = 6.85KK419 pKa = 10.59FVDD422 pKa = 3.2SRR424 pKa = 11.84EE425 pKa = 4.27YY426 pKa = 10.33QDD428 pKa = 4.36HH429 pKa = 7.44ADD431 pKa = 5.2FKK433 pKa = 11.35VEE435 pKa = 3.41WEE437 pKa = 4.01EE438 pKa = 3.77RR439 pKa = 11.84RR440 pKa = 11.84KK441 pKa = 10.42EE442 pKa = 4.01EE443 pKa = 4.11RR444 pKa = 11.84DD445 pKa = 2.97EE446 pKa = 4.88DD447 pKa = 3.75YY448 pKa = 10.96TYY450 pKa = 11.85GLVHH454 pKa = 6.83NKK456 pKa = 9.69RR457 pKa = 11.84FIEE460 pKa = 3.65MRR462 pKa = 11.84YY463 pKa = 9.43RR464 pKa = 11.84KK465 pKa = 10.01SFGDD469 pKa = 3.42ATIVNASEE477 pKa = 3.69EE478 pKa = 4.24TTRR481 pKa = 11.84NFFNNKK487 pKa = 9.04ALPAGVRR494 pKa = 11.84WADD497 pKa = 3.28EE498 pKa = 4.07DD499 pKa = 4.66DD500 pKa = 4.49YY501 pKa = 11.46EE502 pKa = 5.75AVMPDD507 pKa = 3.94FVHH510 pKa = 7.56ASKK513 pKa = 10.78KK514 pKa = 7.96PQLRR518 pKa = 11.84GTEE521 pKa = 4.44TTCGMTQKK529 pKa = 10.8SSGPPPLVTSAPASVSSSSEE549 pKa = 3.75KK550 pKa = 10.62AKK552 pKa = 10.6PSAKK556 pKa = 8.48PTSPPTSILKK566 pKa = 9.61PSPKK570 pKa = 10.01LSSNDD575 pKa = 3.37QSSKK579 pKa = 9.48PSVVFKK585 pKa = 10.5EE586 pKa = 4.13VEE588 pKa = 4.02MQKK591 pKa = 10.56NSPSTSKK598 pKa = 10.01PASSGQPKK606 pKa = 9.73SRR608 pKa = 11.84KK609 pKa = 8.62SKK611 pKa = 10.56KK612 pKa = 9.58KK613 pKa = 8.3EE614 pKa = 3.45HH615 pKa = 6.14SQAFSAIIQNLEE627 pKa = 4.0SLSDD631 pKa = 3.74SEE633 pKa = 5.11VRR635 pKa = 11.84DD636 pKa = 3.46LKK638 pKa = 11.34SRR640 pKa = 11.84IGG642 pKa = 3.39

Molecular weight: 71.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEZ9|A0A1L3KEZ9_9VIRU Peptidase S39 domain-containing protein OS=Hubei sobemo-like virus 2 OX=1923205 PE=4 SV=1
MM1 pKa = 7.54LNHH4 pKa = 7.55ASRR7 pKa = 11.84NLRR10 pKa = 11.84NSPMSTDD17 pKa = 3.2TFRR20 pKa = 11.84TSKK23 pKa = 10.52KK24 pKa = 9.22RR25 pKa = 11.84WPLFAPCLGGAGKK38 pKa = 10.63RR39 pKa = 11.84MPTKK43 pKa = 10.52RR44 pKa = 11.84KK45 pKa = 7.62QTSKK49 pKa = 10.43KK50 pKa = 10.12AKK52 pKa = 8.16STAKK56 pKa = 10.09PRR58 pKa = 11.84VQQRR62 pKa = 11.84QLAISQRR69 pKa = 11.84RR70 pKa = 11.84ARR72 pKa = 11.84PDD74 pKa = 2.96VGHH77 pKa = 6.83IVHH80 pKa = 6.74KK81 pKa = 10.91VCALTDD87 pKa = 4.1PFCPAAVGAKK97 pKa = 8.62WPNSTAIRR105 pKa = 11.84TLAYY109 pKa = 8.63PFHH112 pKa = 7.0SRR114 pKa = 11.84RR115 pKa = 11.84TVAVTATGAGAVIFTPGYY133 pKa = 9.79NNWTAGATIDD143 pKa = 4.97AEE145 pKa = 4.4TGNALFTNYY154 pKa = 8.07ATAPKK159 pKa = 10.23DD160 pKa = 2.87IGAALWRR167 pKa = 11.84IVSWGFKK174 pKa = 8.89IRR176 pKa = 11.84SVASPMLASGMVRR189 pKa = 11.84VRR191 pKa = 11.84IFAPAYY197 pKa = 10.2SGANNIDD204 pKa = 3.46PTSYY208 pKa = 10.53LCEE211 pKa = 4.34DD212 pKa = 5.36FIDD215 pKa = 5.91VPLQDD220 pKa = 4.42CKK222 pKa = 10.37EE223 pKa = 4.13LCVIPQRR230 pKa = 11.84SGEE233 pKa = 3.9LAEE236 pKa = 4.75IFNASDD242 pKa = 3.13VGGATIQSTANVGWPYY258 pKa = 11.41VLVSVDD264 pKa = 3.63GAPASTVPLDD274 pKa = 3.03IEE276 pKa = 4.4VYY278 pKa = 10.31YY279 pKa = 10.76NYY281 pKa = 10.67EE282 pKa = 4.22LMFSDD287 pKa = 5.07GNALSMLMTPSPSASGLMTQAASYY311 pKa = 7.37VTKK314 pKa = 10.78SLGSFVKK321 pKa = 10.39QGAKK325 pKa = 9.1QVEE328 pKa = 4.82GAVKK332 pKa = 8.8TAASRR337 pKa = 11.84YY338 pKa = 9.33IGGLLGGPMGAAAGGMLAITVDD360 pKa = 3.34

Molecular weight: 38.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1355

353

642

451.7

49.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.52 ± 1.639

1.328 ± 0.43

5.092 ± 0.581

5.609 ± 1.388

3.616 ± 0.106

6.199 ± 0.897

1.845 ± 0.278

3.838 ± 0.605

6.273 ± 0.482

8.118 ± 0.653

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.423

4.207 ± 0.222

6.125 ± 0.691

3.469 ± 0.155

5.018 ± 0.199

9.299 ± 0.941

5.83 ± 0.928

7.306 ± 0.646

1.624 ± 0.146

3.1 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski