Wuhan horsefly Virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Horwuvirus; Horsefly horwuvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KY39|A0A0B5KY39_9VIRU Glycoprotein OS=Wuhan horsefly Virus OX=1608139 GN=G PE=4 SV=1
MM1 pKa = 7.32GCLRR5 pKa = 11.84FIIIVISILIVNKK18 pKa = 9.82RR19 pKa = 11.84VCSMEE24 pKa = 3.71DD25 pKa = 3.01RR26 pKa = 11.84EE27 pKa = 4.91IIVHH31 pKa = 5.8LTNKK35 pKa = 9.37VVEE38 pKa = 4.62DD39 pKa = 3.54ATIQDD44 pKa = 3.35KK45 pKa = 10.44TEE47 pKa = 3.65FLIYY51 pKa = 10.76LNDD54 pKa = 3.6ITSLDD59 pKa = 3.47PWYY62 pKa = 10.27HH63 pKa = 6.41QIGCKK68 pKa = 9.04TNNCGLRR75 pKa = 11.84YY76 pKa = 10.21NNNSKK81 pKa = 10.55QYY83 pKa = 10.93DD84 pKa = 3.28IALSQKK90 pKa = 9.9NVNLGWFYY98 pKa = 11.33GSKK101 pKa = 10.38ALKK104 pKa = 10.26DD105 pKa = 3.39KK106 pKa = 9.13PTYY109 pKa = 10.2PGVDD113 pKa = 3.45CLTPFIVINDD123 pKa = 4.21KK124 pKa = 9.32ITYY127 pKa = 9.63VPEE130 pKa = 4.15LLVKK134 pKa = 9.48FIVSICSSDD143 pKa = 3.33RR144 pKa = 11.84GRR146 pKa = 11.84KK147 pKa = 8.9CKK149 pKa = 10.64ACDD152 pKa = 3.6NNCVYY157 pKa = 10.65QSDD160 pKa = 5.19LINFGLMRR168 pKa = 11.84TNCQLVIVEE177 pKa = 4.59NNTTIPYY184 pKa = 10.31DD185 pKa = 4.09VIDD188 pKa = 3.62SPYY191 pKa = 9.6TYY193 pKa = 10.54EE194 pKa = 4.3DD195 pKa = 3.17KK196 pKa = 11.07CVNGEE201 pKa = 3.5ICMDD205 pKa = 3.5STIEE209 pKa = 4.07SLCYY213 pKa = 10.12SGSCTEE219 pKa = 4.58IKK221 pKa = 10.6DD222 pKa = 3.91NQPEE226 pKa = 4.19KK227 pKa = 10.53CVRR230 pKa = 11.84IPWKK234 pKa = 9.26YY235 pKa = 8.35PKK237 pKa = 10.54YY238 pKa = 10.68GIIEE242 pKa = 4.1VKK244 pKa = 10.28LQNNKK249 pKa = 8.07TSQMTCKK256 pKa = 9.78IDD258 pKa = 4.24DD259 pKa = 3.87FCTLIRR265 pKa = 11.84SYY267 pKa = 11.04TYY269 pKa = 7.33FTPLLHH275 pKa = 7.01PYY277 pKa = 10.23CSSTKK282 pKa = 10.06EE283 pKa = 3.63KK284 pKa = 11.04VIFYY288 pKa = 9.11TLFIMLIWSVLILTIQFKK306 pKa = 10.34LYY308 pKa = 10.14KK309 pKa = 10.02VLYY312 pKa = 8.61PCYY315 pKa = 10.51KK316 pKa = 9.68LTSCLKK322 pKa = 9.96FEE324 pKa = 4.08IMKK327 pKa = 10.25YY328 pKa = 9.77LYY330 pKa = 8.88EE331 pKa = 3.9RR332 pKa = 11.84HH333 pKa = 6.23SIEE336 pKa = 4.88IEE338 pKa = 3.8MDD340 pKa = 3.52VLNSDD345 pKa = 3.4GSARR349 pKa = 11.84KK350 pKa = 9.22GKK352 pKa = 10.11KK353 pKa = 9.09SKK355 pKa = 10.27SWLKK359 pKa = 10.47QLLLLIFCLQRR370 pKa = 11.84VEE372 pKa = 4.56SSCDD376 pKa = 3.18FAFPIHH382 pKa = 7.09SSTSVCINGSCDD394 pKa = 2.98EE395 pKa = 4.45MEE397 pKa = 4.32FMSYY401 pKa = 10.09HH402 pKa = 5.53VPSGNLHH409 pKa = 5.5ICGNNIDD416 pKa = 3.84IYY418 pKa = 11.02IMKK421 pKa = 9.5TYY423 pKa = 10.08LRR425 pKa = 11.84CVPDD429 pKa = 3.19ILYY432 pKa = 7.37YY433 pKa = 10.32TPITIIKK440 pKa = 10.24ASRR443 pKa = 11.84TWNCLWAGDD452 pKa = 4.27CTNTCNSSVINHH464 pKa = 7.2EE465 pKa = 4.22INEE468 pKa = 3.98KK469 pKa = 10.25DD470 pKa = 3.36QYY472 pKa = 10.45VHH474 pKa = 6.7LRR476 pKa = 11.84SCEE479 pKa = 3.96KK480 pKa = 10.69VPGSGNVCRR489 pKa = 11.84YY490 pKa = 10.7DD491 pKa = 5.27DD492 pKa = 3.5ICVWRR497 pKa = 11.84NIFIAGKK504 pKa = 10.28DD505 pKa = 3.32NGLKK509 pKa = 10.04FLRR512 pKa = 11.84VSSCTEE518 pKa = 3.37HH519 pKa = 8.03LYY521 pKa = 10.88FSSLLIKK528 pKa = 10.51SEE530 pKa = 3.99NQSFNLMAEE539 pKa = 3.99HH540 pKa = 6.14GTLYY544 pKa = 11.15NLGDD548 pKa = 3.7KK549 pKa = 11.03LSFQIINTNSFRR561 pKa = 11.84KK562 pKa = 9.64DD563 pKa = 3.29LKK565 pKa = 11.02LLEE568 pKa = 5.27LGDD571 pKa = 3.72KK572 pKa = 11.0GYY574 pKa = 11.0LGDD577 pKa = 4.6ANNLHH582 pKa = 6.58EE583 pKa = 4.75SVRR586 pKa = 11.84HH587 pKa = 5.14KK588 pKa = 10.44PGSIQCEE595 pKa = 3.97DD596 pKa = 3.05SDD598 pKa = 4.56IYY600 pKa = 11.45SNCRR604 pKa = 11.84WNKK607 pKa = 7.8EE608 pKa = 3.79SEE610 pKa = 4.14MFDD613 pKa = 3.35GGFQEE618 pKa = 5.64HH619 pKa = 6.21YY620 pKa = 10.87LVDD623 pKa = 4.5NEE625 pKa = 5.3DD626 pKa = 3.16KK627 pKa = 10.73PQRR630 pKa = 11.84LDD632 pKa = 3.37EE633 pKa = 4.44AFKK636 pKa = 10.36KK637 pKa = 10.25ARR639 pKa = 11.84HH640 pKa = 5.82IVTADD645 pKa = 3.05GVEE648 pKa = 4.13IEE650 pKa = 4.61NLPFTLMVSNRR661 pKa = 11.84NHH663 pKa = 6.65LKK665 pKa = 10.86DD666 pKa = 5.14KK667 pKa = 10.81ISLDD671 pKa = 3.65LGEE674 pKa = 4.32LTIANGFVEE683 pKa = 4.45GLSYY687 pKa = 11.28LDD689 pKa = 4.52NKK691 pKa = 10.64CKK693 pKa = 10.17MSFTYY698 pKa = 10.7SLTEE702 pKa = 3.51QMVIQISSPIITSSILKK719 pKa = 9.77LDD721 pKa = 3.48KK722 pKa = 11.1AKK724 pKa = 10.7EE725 pKa = 4.17SEE727 pKa = 4.04LSINCTCLYY736 pKa = 10.77SEE738 pKa = 5.01VDD740 pKa = 3.25TTQITFSAYY749 pKa = 9.76HH750 pKa = 6.4LSKK753 pKa = 9.96TYY755 pKa = 8.8TFKK758 pKa = 10.98TNLSHH763 pKa = 7.87PIGHH767 pKa = 6.15FTQYY771 pKa = 11.29HH772 pKa = 6.99DD773 pKa = 3.5IFLGKK778 pKa = 10.15SLGGYY783 pKa = 10.24DD784 pKa = 3.66YY785 pKa = 11.25FSGLSHH791 pKa = 7.14FFSSHH796 pKa = 5.56SDD798 pKa = 3.84FIYY801 pKa = 10.62SIVGVIIVLFLLKK814 pKa = 10.74LFLCC818 pKa = 5.11

Molecular weight: 93.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KU60|A0A0B5KU60_9VIRU Nucleocapsid protein OS=Wuhan horsefly Virus OX=1608139 GN=N PE=4 SV=1
MM1 pKa = 7.37LKK3 pKa = 10.23KK4 pKa = 10.39YY5 pKa = 10.71LKK7 pKa = 10.32EE8 pKa = 3.78KK9 pKa = 10.38SKK11 pKa = 10.96KK12 pKa = 9.69DD13 pKa = 3.29KK14 pKa = 10.72KK15 pKa = 10.72RR16 pKa = 11.84EE17 pKa = 3.84NTLVVSTAGEE27 pKa = 4.02ASHH30 pKa = 6.8SRR32 pKa = 11.84GAIPKK37 pKa = 5.74TQRR40 pKa = 11.84IIRR43 pKa = 11.84YY44 pKa = 6.61SQRR47 pKa = 11.84EE48 pKa = 4.16LKK50 pKa = 10.34EE51 pKa = 3.46QDD53 pKa = 4.75KK54 pKa = 11.0ILQQKK59 pKa = 9.95NNGDD63 pKa = 3.84IGNMDD68 pKa = 3.81SVRR71 pKa = 11.84EE72 pKa = 4.07RR73 pKa = 11.84IFEE76 pKa = 3.82SHH78 pKa = 5.64YY79 pKa = 10.85RR80 pKa = 11.84SMEE83 pKa = 4.03TTPSEE88 pKa = 4.03LKK90 pKa = 10.99GMLEE94 pKa = 3.97LLSYY98 pKa = 9.82YY99 pKa = 9.75PRR101 pKa = 11.84APTEE105 pKa = 4.03TFTSLKK111 pKa = 9.79ISSPVQVTDD120 pKa = 3.65GLSIMEE126 pKa = 4.98IINGEE131 pKa = 4.22RR132 pKa = 11.84ISTGWKK138 pKa = 9.37HH139 pKa = 5.43PVGFVGFPRR148 pKa = 11.84IIFIFNACSPDD159 pKa = 3.69DD160 pKa = 4.25PGVIIVSLTHH170 pKa = 6.85KK171 pKa = 10.39GIKK174 pKa = 9.65DD175 pKa = 3.4PCGATLKK182 pKa = 10.67RR183 pKa = 11.84FTGPVNKK190 pKa = 8.6TWSIAFSLKK199 pKa = 10.64HH200 pKa = 5.21MVAIQDD206 pKa = 3.51IEE208 pKa = 3.97KK209 pKa = 10.59LQIHH213 pKa = 6.4CLVEE217 pKa = 3.96DD218 pKa = 3.95SNRR221 pKa = 11.84EE222 pKa = 3.62DD223 pKa = 3.74SVIGKK228 pKa = 9.88LDD230 pKa = 3.29MHH232 pKa = 6.5FTLDD236 pKa = 4.13LSTTPMIINYY246 pKa = 9.38PEE248 pKa = 3.63TTYY251 pKa = 10.87YY252 pKa = 9.74IHH254 pKa = 7.93PEE256 pKa = 3.99LDD258 pKa = 3.2VKK260 pKa = 10.99KK261 pKa = 10.54YY262 pKa = 10.38IEE264 pKa = 5.11EE265 pKa = 3.81IDD267 pKa = 3.42KK268 pKa = 11.35ASIKK272 pKa = 10.35KK273 pKa = 9.76FRR275 pKa = 11.84EE276 pKa = 3.46EE277 pKa = 4.0DD278 pKa = 3.19EE279 pKa = 4.55ARR281 pKa = 11.84LLRR284 pKa = 11.84TRR286 pKa = 11.84NYY288 pKa = 10.42AISKK292 pKa = 7.99STGVDD297 pKa = 3.44DD298 pKa = 5.72SIHH301 pKa = 5.95IQPSAPMNEE310 pKa = 3.92NEE312 pKa = 5.15KK313 pKa = 10.57IIINLDD319 pKa = 3.29PKK321 pKa = 10.64IDD323 pKa = 3.92DD324 pKa = 4.17GFNLKK329 pKa = 10.25SNKK332 pKa = 9.79GADD335 pKa = 3.79SLHH338 pKa = 5.22TTQEE342 pKa = 3.64EE343 pKa = 4.35EE344 pKa = 3.79IRR346 pKa = 11.84RR347 pKa = 11.84KK348 pKa = 10.3LRR350 pKa = 11.84DD351 pKa = 3.03LAIRR355 pKa = 11.84GKK357 pKa = 10.17KK358 pKa = 7.22QQ359 pKa = 2.74

Molecular weight: 40.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4747

359

3117

1186.8

137.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.097 ± 0.448

1.896 ± 0.742

5.751 ± 0.15

7.183 ± 0.557

4.761 ± 0.28

3.855 ± 0.493

2.359 ± 0.176

9.164 ± 0.542

8.237 ± 0.312

9.585 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.336

5.898 ± 0.277

3.476 ± 0.322

3.16 ± 0.282

4.403 ± 0.393

8.995 ± 0.186

5.309 ± 0.312

4.803 ± 0.515

0.969 ± 0.088

4.171 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski