Hubei tombus-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGY5|A0A1L3KGY5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 8 OX=1923295 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84AVDD5 pKa = 3.17RR6 pKa = 11.84ALRR9 pKa = 11.84EE10 pKa = 3.63RR11 pKa = 11.84YY12 pKa = 9.52FFVKK16 pKa = 9.44TKK18 pKa = 10.88KK19 pKa = 10.42EE20 pKa = 4.12GFQPALPVRR29 pKa = 11.84SRR31 pKa = 11.84AYY33 pKa = 8.7EE34 pKa = 3.86TDD36 pKa = 2.68VHH38 pKa = 6.48LRR40 pKa = 11.84SFRR43 pKa = 11.84AQVVASCKK51 pKa = 10.55APVVSIADD59 pKa = 3.48VVNSYY64 pKa = 9.11TGPKK68 pKa = 9.36RR69 pKa = 11.84EE70 pKa = 4.59AYY72 pKa = 9.76AKK74 pKa = 10.57AAISLMKK81 pKa = 10.53DD82 pKa = 3.06AVNHH86 pKa = 5.64QDD88 pKa = 2.92ASLMMFGKK96 pKa = 10.4FEE98 pKa = 4.03KK99 pKa = 10.62QNLDD103 pKa = 3.06KK104 pKa = 11.1ANRR107 pKa = 11.84GINPRR112 pKa = 11.84SKK114 pKa = 10.52RR115 pKa = 11.84YY116 pKa = 8.82NLEE119 pKa = 3.54LGRR122 pKa = 11.84YY123 pKa = 8.07LKK125 pKa = 10.8FMEE128 pKa = 4.72KK129 pKa = 9.92PLYY132 pKa = 10.2RR133 pKa = 11.84SINKK137 pKa = 10.01AYY139 pKa = 10.21GARR142 pKa = 11.84TAHH145 pKa = 5.47TVIKK149 pKa = 9.51GLNVADD155 pKa = 4.09SAAVAHH161 pKa = 6.37AKK163 pKa = 8.94WKK165 pKa = 9.89LFKK168 pKa = 10.84RR169 pKa = 11.84PVAVGLDD176 pKa = 3.5AEE178 pKa = 4.74KK179 pKa = 10.68FDD181 pKa = 3.67AHH183 pKa = 7.66VSIPCLRR190 pKa = 11.84YY191 pKa = 8.2EE192 pKa = 3.98HH193 pKa = 6.81SFYY196 pKa = 11.16LQIHH200 pKa = 5.4SRR202 pKa = 11.84QVKK205 pKa = 8.37RR206 pKa = 11.84QMMLKK211 pKa = 9.89QLLNWQLVNTGVAYY225 pKa = 10.87CDD227 pKa = 3.36DD228 pKa = 3.92GKK230 pKa = 11.02IKK232 pKa = 10.64FKK234 pKa = 10.89IKK236 pKa = 9.14GTRR239 pKa = 11.84CSGDD243 pKa = 3.56LNTSMGNCLIMCGLVYY259 pKa = 10.26AYY261 pKa = 9.75SASMGVNVEE270 pKa = 4.08LMNNGDD276 pKa = 3.85DD277 pKa = 3.65CVVIMEE283 pKa = 4.49EE284 pKa = 4.09EE285 pKa = 4.06DD286 pKa = 3.51VEE288 pKa = 4.92KK289 pKa = 11.07YY290 pKa = 9.8VAGVPEE296 pKa = 4.01YY297 pKa = 10.48FKK299 pKa = 11.11RR300 pKa = 11.84KK301 pKa = 7.68GFRR304 pKa = 11.84MTVEE308 pKa = 3.82EE309 pKa = 4.17PVRR312 pKa = 11.84EE313 pKa = 3.93FEE315 pKa = 5.1QIEE318 pKa = 4.74FCQSHH323 pKa = 6.52PVHH326 pKa = 7.09DD327 pKa = 4.16GEE329 pKa = 4.78KK330 pKa = 9.28YY331 pKa = 10.19VMVRR335 pKa = 11.84NLTNCLQKK343 pKa = 11.17DD344 pKa = 4.0PMCLVPIQNAKK355 pKa = 10.38ALQQWYY361 pKa = 9.09DD362 pKa = 3.43AVGSCGLSLTPGIPVLQEE380 pKa = 3.61FYY382 pKa = 10.0KK383 pKa = 9.73TFKK386 pKa = 10.66RR387 pKa = 11.84SGRR390 pKa = 11.84PCRR393 pKa = 11.84EE394 pKa = 3.52GFKK397 pKa = 10.17RR398 pKa = 11.84TVFKK402 pKa = 10.13NTSYY406 pKa = 11.03YY407 pKa = 10.71EE408 pKa = 3.97RR409 pKa = 11.84VKK411 pKa = 11.1DD412 pKa = 3.45LTGRR416 pKa = 11.84EE417 pKa = 4.28LSCSSVSRR425 pKa = 11.84SSFYY429 pKa = 10.65FAFGVLPEE437 pKa = 4.02HH438 pKa = 6.56QIAIEE443 pKa = 4.17AYY445 pKa = 7.87YY446 pKa = 11.39ASMTLSAVVEE456 pKa = 4.41DD457 pKa = 4.42AMHH460 pKa = 7.28PEE462 pKa = 3.74YY463 pKa = 10.69AYY465 pKa = 10.81DD466 pKa = 5.4KK467 pKa = 11.2GDD469 pKa = 3.55NCCPPVVDD477 pKa = 6.16CIFAA481 pKa = 4.4

Molecular weight: 54.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGY5|A0A1L3KGY5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 8 OX=1923295 PE=4 SV=1
MM1 pKa = 7.39QNNFYY6 pKa = 10.45EE7 pKa = 4.33KK8 pKa = 10.69RR9 pKa = 11.84EE10 pKa = 3.9FQVNEE15 pKa = 3.34VSARR19 pKa = 11.84HH20 pKa = 4.13TRR22 pKa = 11.84EE23 pKa = 3.63RR24 pKa = 11.84RR25 pKa = 11.84NLWAEE30 pKa = 3.57KK31 pKa = 9.27WDD33 pKa = 3.78SFKK36 pKa = 11.44SGFAEE41 pKa = 4.18MYY43 pKa = 7.09YY44 pKa = 10.45TSTFFNLLCCTVPEE58 pKa = 4.12DD59 pKa = 3.77HH60 pKa = 7.53LEE62 pKa = 4.26FKK64 pKa = 10.77LDD66 pKa = 3.46QADD69 pKa = 3.79RR70 pKa = 11.84LAVRR74 pKa = 11.84KK75 pKa = 9.82ALMQSLDD82 pKa = 3.61YY83 pKa = 10.46GAHH86 pKa = 6.36KK87 pKa = 10.51SIIAATMEE95 pKa = 5.31DD96 pKa = 3.39ISEE99 pKa = 4.31HH100 pKa = 5.97EE101 pKa = 4.64GYY103 pKa = 10.2EE104 pKa = 3.92MRR106 pKa = 11.84MDD108 pKa = 4.03PPVKK112 pKa = 9.75QVPVPSSSSTALIVRR127 pKa = 11.84NVNLQPQGAVMLKK140 pKa = 9.83PVKK143 pKa = 9.99GVQGVRR149 pKa = 11.84SIPRR153 pKa = 11.84FTAAVVVALRR163 pKa = 11.84GKK165 pKa = 10.21LGQLNPSVQGNEE177 pKa = 3.71LVLEE181 pKa = 4.34RR182 pKa = 11.84EE183 pKa = 4.89ALRR186 pKa = 11.84MMRR189 pKa = 11.84DD190 pKa = 3.46YY191 pKa = 11.23NVRR194 pKa = 11.84EE195 pKa = 3.98VDD197 pKa = 3.74RR198 pKa = 11.84AVHH201 pKa = 6.35LPRR204 pKa = 11.84VMAAYY209 pKa = 9.15WSADD213 pKa = 2.9VHH215 pKa = 6.03YY216 pKa = 9.89RR217 pKa = 11.84VPTVEE222 pKa = 3.94SRR224 pKa = 11.84MSRR227 pKa = 11.84FQRR230 pKa = 11.84WLLNRR235 pKa = 11.84PSSSPQYY242 pKa = 10.53AVPRR246 pKa = 11.84AA247 pKa = 3.38

Molecular weight: 28.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1070

247

481

356.7

39.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.159 ± 0.492

2.15 ± 0.63

4.112 ± 0.353

5.327 ± 0.801

4.393 ± 0.271

6.168 ± 1.081

1.776 ± 0.432

3.458 ± 0.511

5.888 ± 0.939

7.196 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.364 ± 0.444

4.953 ± 0.53

4.86 ± 0.377

3.645 ± 0.425

6.542 ± 1.144

6.822 ± 0.348

5.888 ± 1.984

8.879 ± 0.676

0.841 ± 0.262

4.579 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski