Mycobacterium phage ByChance

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HBY5|A0A2Z5HBY5_9CAUD Uncharacterized protein OS=Mycobacterium phage ByChance OX=2250373 GN=14 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 10.81FEE5 pKa = 4.22ALVTEE10 pKa = 4.79TLRR13 pKa = 11.84QLGWTCEE20 pKa = 3.89YY21 pKa = 10.54HH22 pKa = 6.96EE23 pKa = 4.88PVSDD27 pKa = 4.26RR28 pKa = 11.84DD29 pKa = 3.55EE30 pKa = 5.32CSQCDD35 pKa = 3.75DD36 pKa = 4.08SHH38 pKa = 7.21DD39 pKa = 3.66KK40 pKa = 9.54TAAALLEE47 pKa = 4.03MLKK50 pKa = 10.73ANRR53 pKa = 11.84IALIEE58 pKa = 4.17LPEE61 pKa = 4.48PYY63 pKa = 9.29FVHH66 pKa = 6.99SATDD70 pKa = 3.58DD71 pKa = 3.66VNGYY75 pKa = 10.69ADD77 pKa = 3.33WEE79 pKa = 4.5YY80 pKa = 10.42PHH82 pKa = 7.59GSIATTDD89 pKa = 4.07DD90 pKa = 3.53GTIMWGQWHH99 pKa = 6.11ITDD102 pKa = 3.99PEE104 pKa = 4.2KK105 pKa = 11.18VRR107 pKa = 11.84AAAAALLAAAAAAEE121 pKa = 4.35VTEE124 pKa = 4.3

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HB47|A0A2Z5HB47_9CAUD Uncharacterized protein OS=Mycobacterium phage ByChance OX=2250373 GN=69 PE=4 SV=1
MM1 pKa = 7.43TFFSGRR7 pKa = 11.84RR8 pKa = 11.84GSARR12 pKa = 11.84HH13 pKa = 5.63GKK15 pKa = 9.34GEE17 pKa = 3.83AWQGSARR24 pKa = 11.84HH25 pKa = 5.73GRR27 pKa = 11.84HH28 pKa = 4.51GSAWRR33 pKa = 11.84GLAGHH38 pKa = 6.47GWARR42 pKa = 11.84RR43 pKa = 11.84GMAGAARR50 pKa = 11.84QGG52 pKa = 3.23

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17297

25

1175

182.1

19.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.771 ± 0.482

1.156 ± 0.17

6.654 ± 0.237

5.804 ± 0.357

2.931 ± 0.174

8.747 ± 0.655

2.139 ± 0.19

4.342 ± 0.194

3.463 ± 0.226

7.296 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.132

3.44 ± 0.175

6.042 ± 0.214

3.498 ± 0.195

6.689 ± 0.398

6.036 ± 0.347

6.712 ± 0.236

7.273 ± 0.335

2.237 ± 0.151

2.44 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski