Rhodobacterales bacterium HTCC2255

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; unclassified Rhodobacterales

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0FDT7|Q0FDT7_9RHOB 3-isopropylmalate dehydratase small subunit OS=Rhodobacterales bacterium HTCC2255 OX=367336 GN=leuD PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.41KK3 pKa = 10.44LLLASTALVATAGMAAADD21 pKa = 3.75VTITGHH27 pKa = 6.36AAAGYY32 pKa = 10.44HH33 pKa = 6.6SDD35 pKa = 4.09LDD37 pKa = 4.11GKK39 pKa = 10.91GVATAATTDD48 pKa = 3.07SGIYY52 pKa = 10.28SNVGVDD58 pKa = 4.05FAMSGATDD66 pKa = 3.52GGLTFTASMNIDD78 pKa = 3.54AGTEE82 pKa = 3.56IDD84 pKa = 4.08SGNFEE89 pKa = 4.46YY90 pKa = 11.0DD91 pKa = 3.2HH92 pKa = 7.08NDD94 pKa = 2.95AGTAGLGAVTISGAFGTLTFDD115 pKa = 4.26NAGIDD120 pKa = 3.55NLYY123 pKa = 10.52NADD126 pKa = 4.09HH127 pKa = 6.88NSHH130 pKa = 6.66DD131 pKa = 3.89VKK133 pKa = 10.99FAGTVNGVAIAVTADD148 pKa = 3.57ADD150 pKa = 3.97DD151 pKa = 4.41SVAGTSGTAASADD164 pKa = 3.6MSASASIASGAMTYY178 pKa = 11.37ALAVSNHH185 pKa = 5.81EE186 pKa = 4.28ADD188 pKa = 4.71GMATNMKK195 pKa = 8.23VTYY198 pKa = 9.87VVNDD202 pKa = 3.77MLTVSASNDD211 pKa = 3.42KK212 pKa = 10.84EE213 pKa = 4.29STAGADD219 pKa = 3.16AVTKK223 pKa = 10.35VAATIVANGFTIGLSSDD240 pKa = 3.18NSASGGDD247 pKa = 3.24WDD249 pKa = 4.36VDD251 pKa = 3.58LGYY254 pKa = 8.99TLSGVALAYY263 pKa = 8.86GTDD266 pKa = 4.1EE267 pKa = 4.08NDD269 pKa = 2.49AWAATASKK277 pKa = 10.67SLGGGATAAAGINWEE292 pKa = 4.13DD293 pKa = 3.3SVYY296 pKa = 11.2AGISFAFF303 pKa = 3.99

Molecular weight: 29.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0FD54|Q0FD54_9RHOB Membrane protein insertase YidC OS=Rhodobacterales bacterium HTCC2255 OX=367336 GN=yidC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 8.58ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84VQGRR39 pKa = 11.84KK40 pKa = 9.39SLSAA44 pKa = 3.83

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2236

0

2236

704440

21

1508

315.0

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.45 ± 0.057

1.034 ± 0.014

5.642 ± 0.04

5.914 ± 0.052

4.338 ± 0.041

7.523 ± 0.039

1.968 ± 0.027

7.893 ± 0.046

5.995 ± 0.044

9.642 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.027

4.879 ± 0.042

4.069 ± 0.032

3.062 ± 0.02

4.561 ± 0.038

6.724 ± 0.038

5.171 ± 0.028

6.317 ± 0.045

1.273 ± 0.02

2.684 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski