Pseudoalteromonas phage vB_PspS-H40/1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173H0S7|A0A173H0S7_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage vB_PspS-H40/1 OX=1856120 GN=H401_63 PE=4 SV=1
MM1 pKa = 7.98PFTLEE6 pKa = 3.86TVKK9 pKa = 10.83AYY11 pKa = 9.68GASGNDD17 pKa = 2.82IFIQAKK23 pKa = 9.13IDD25 pKa = 3.72AFADD29 pKa = 3.82TYY31 pKa = 11.1TCLTTSYY38 pKa = 10.32SIAIADD44 pKa = 5.15DD45 pKa = 3.31IANSYY50 pKa = 9.18VAGTIQEE57 pKa = 4.49SGGEE61 pKa = 4.02AQVTSTKK68 pKa = 10.52APNGASTNFKK78 pKa = 9.31QSKK81 pKa = 9.29YY82 pKa = 10.09GDD84 pKa = 3.38SGEE87 pKa = 4.24YY88 pKa = 10.92ANGLLANAYY97 pKa = 10.54LNDD100 pKa = 3.99ANYY103 pKa = 10.52CLPIDD108 pKa = 4.33SNDD111 pKa = 3.18FFIGVAGSTYY121 pKa = 10.53EE122 pKa = 4.33ADD124 pKa = 3.63NPQQ127 pKa = 2.89

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173H0T9|A0A173H0T9_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage vB_PspS-H40/1 OX=1856120 GN=H401_68 PE=4 SV=1
MM1 pKa = 7.47NNMSDD6 pKa = 3.95LSFTQTSRR14 pKa = 11.84AMRR17 pKa = 11.84STHH20 pKa = 6.46LSRR23 pKa = 11.84INHH26 pKa = 6.48EE27 pKa = 4.14YY28 pKa = 10.22RR29 pKa = 11.84PIWVIAATNNAKK41 pKa = 9.65PKK43 pKa = 10.32KK44 pKa = 9.84VIDD47 pKa = 3.47RR48 pKa = 11.84RR49 pKa = 11.84AIGWKK54 pKa = 8.91SQPNVFMGCC63 pKa = 3.33

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14189

50

833

194.4

21.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.964 ± 0.489

1.339 ± 0.136

6.357 ± 0.212

6.54 ± 0.231

4.031 ± 0.164

6.801 ± 0.249

1.663 ± 0.18

6.695 ± 0.218

7.069 ± 0.37

7.464 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.138

5.673 ± 0.228

3.439 ± 0.202

3.799 ± 0.211

3.989 ± 0.199

7.041 ± 0.234

5.976 ± 0.307

6.378 ± 0.203

1.339 ± 0.142

3.975 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski