Amino acid dipepetide frequency for Rhizopus microsporus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.309AlaAla: 5.309 ± 0.052	1.007AlaCys: 1.007 ± 0.016	2.992AlaAsp: 2.992 ± 0.026	3.769AlaGlu: 3.769 ± 0.035	2.694AlaPhe: 2.694 ± 0.025	3.113AlaGly: 3.113 ± 0.033	1.382AlaHis: 1.382 ± 0.018	4.409AlaIle: 4.409 ± 0.039	4.148AlaLys: 4.148 ± 0.036	6.206AlaLeu: 6.206 ± 0.046	1.611AlaMet: 1.611 ± 0.019	2.842AlaAsn: 2.842 ± 0.027	2.659AlaPro: 2.659 ± 0.027	2.55AlaGln: 2.55 ± 0.03	3.018AlaArg: 3.018 ± 0.029	5.139AlaSer: 5.139 ± 0.039	4.008AlaThr: 4.008 ± 0.04	4.026AlaVal: 4.026 ± 0.041	0.7AlaTrp: 0.7 ± 0.013	2.207AlaTyr: 2.207 ± 0.026	0.0AlaXaa: 0.0 ± 0.0
Cys	0.771CysAla: 0.771 ± 0.014	0.333CysCys: 0.333 ± 0.01	0.716CysAsp: 0.716 ± 0.012	0.686CysGlu: 0.686 ± 0.013	0.712CysPhe: 0.712 ± 0.014	0.867CysGly: 0.867 ± 0.018	0.447CysHis: 0.447 ± 0.011	1.109CysIle: 1.109 ± 0.018	0.856CysLys: 0.856 ± 0.017	1.558CysLeu: 1.558 ± 0.024	0.359CysMet: 0.359 ± 0.01	0.66CysAsn: 0.66 ± 0.013	0.706CysPro: 0.706 ± 0.013	0.65CysGln: 0.65 ± 0.014	0.755CysArg: 0.755 ± 0.014	1.142CysSer: 1.142 ± 0.019	0.795CysThr: 0.795 ± 0.015	0.886CysVal: 0.886 ± 0.014	0.186CysTrp: 0.186 ± 0.006	0.554CysTyr: 0.554 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.347AspAla: 3.347 ± 0.03	0.716AspCys: 0.716 ± 0.013	4.224AspAsp: 4.224 ± 0.045	4.544AspGlu: 4.544 ± 0.043	2.245AspPhe: 2.245 ± 0.024	2.614AspGly: 2.614 ± 0.026	1.394AspHis: 1.394 ± 0.017	4.224AspIle: 4.224 ± 0.035	3.388AspLys: 3.388 ± 0.032	5.031AspLeu: 5.031 ± 0.036	1.495AspMet: 1.495 ± 0.02	2.803AspAsn: 2.803 ± 0.026	2.587AspPro: 2.587 ± 0.028	2.265AspGln: 2.265 ± 0.025	2.488AspArg: 2.488 ± 0.028	3.617AspSer: 3.617 ± 0.035	3.229AspThr: 3.229 ± 0.028	3.325AspVal: 3.325 ± 0.029	0.722AspTrp: 0.722 ± 0.014	2.144AspTyr: 2.144 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	4.309GluAla: 4.309 ± 0.035	0.8GluCys: 0.8 ± 0.015	4.206GluAsp: 4.206 ± 0.042	6.924GluGlu: 6.924 ± 0.08	2.282GluPhe: 2.282 ± 0.026	2.786GluGly: 2.786 ± 0.028	1.595GluHis: 1.595 ± 0.023	3.918GluIle: 3.918 ± 0.037	5.124GluLys: 5.124 ± 0.045	5.941GluLeu: 5.941 ± 0.048	1.52GluMet: 1.52 ± 0.019	3.152GluAsn: 3.152 ± 0.032	2.339GluPro: 2.339 ± 0.028	3.406GluGln: 3.406 ± 0.033	3.607GluArg: 3.607 ± 0.035	4.307GluSer: 4.307 ± 0.039	3.472GluThr: 3.472 ± 0.035	3.171GluVal: 3.171 ± 0.034	0.752GluTrp: 0.752 ± 0.012	2.374GluTyr: 2.374 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	2.512PheAla: 2.512 ± 0.031	0.696PheCys: 0.696 ± 0.013	2.557PheAsp: 2.557 ± 0.025	2.513PheGlu: 2.513 ± 0.022	1.823PhePhe: 1.823 ± 0.027	2.494PheGly: 2.494 ± 0.03	0.959PheHis: 0.959 ± 0.014	2.835PheIle: 2.835 ± 0.03	2.399PheLys: 2.399 ± 0.026	3.69PheLeu: 3.69 ± 0.033	1.092PheMet: 1.092 ± 0.018	1.94PheAsn: 1.94 ± 0.024	1.615PhePro: 1.615 ± 0.019	1.537PheGln: 1.537 ± 0.02	1.739PheArg: 1.739 ± 0.022	3.084PheSer: 3.084 ± 0.028	2.301PheThr: 2.301 ± 0.026	2.613PheVal: 2.613 ± 0.025	0.559PheTrp: 0.559 ± 0.011	1.574PheTyr: 1.574 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	2.961GlyAla: 2.961 ± 0.033	0.774GlyCys: 0.774 ± 0.016	2.442GlyAsp: 2.442 ± 0.027	2.495GlyGlu: 2.495 ± 0.027	2.267GlyPhe: 2.267 ± 0.027	3.019GlyGly: 3.019 ± 0.036	1.394GlyHis: 1.394 ± 0.021	3.356GlyIle: 3.356 ± 0.036	3.169GlyLys: 3.169 ± 0.027	4.515GlyLeu: 4.515 ± 0.04	1.126GlyMet: 1.126 ± 0.017	2.237GlyAsn: 2.237 ± 0.026	1.736GlyPro: 1.736 ± 0.022	1.986GlyGln: 1.986 ± 0.022	2.46GlyArg: 2.46 ± 0.029	3.809GlySer: 3.809 ± 0.037	2.854GlyThr: 2.854 ± 0.029	3.067GlyVal: 3.067 ± 0.035	0.706GlyTrp: 0.706 ± 0.014	2.107GlyTyr: 2.107 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	1.469HisAla: 1.469 ± 0.02	0.444HisCys: 0.444 ± 0.011	1.328HisAsp: 1.328 ± 0.018	1.412HisGlu: 1.412 ± 0.019	1.1HisPhe: 1.1 ± 0.018	1.29HisGly: 1.29 ± 0.022	1.014HisHis: 1.014 ± 0.022	1.711HisIle: 1.711 ± 0.02	1.39HisLys: 1.39 ± 0.021	2.443HisLeu: 2.443 ± 0.024	0.626HisMet: 0.626 ± 0.011	1.103HisAsn: 1.103 ± 0.016	1.469HisPro: 1.469 ± 0.022	1.283HisGln: 1.283 ± 0.022	1.383HisArg: 1.383 ± 0.021	1.907HisSer: 1.907 ± 0.023	1.402HisThr: 1.402 ± 0.019	1.56HisVal: 1.56 ± 0.017	0.323HisTrp: 0.323 ± 0.01	0.939HisTyr: 0.939 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.155IleAla: 4.155 ± 0.034	1.037IleCys: 1.037 ± 0.016	3.877IleAsp: 3.877 ± 0.033	4.321IleGlu: 4.321 ± 0.037	2.467IlePhe: 2.467 ± 0.026	3.101IleGly: 3.101 ± 0.032	1.814IleHis: 1.814 ± 0.021	3.915IleIle: 3.915 ± 0.036	4.257IleLys: 4.257 ± 0.036	5.807IleLeu: 5.807 ± 0.046	1.497IleMet: 1.497 ± 0.018	2.899IleAsn: 2.899 ± 0.025	3.263IlePro: 3.263 ± 0.031	3.064IleGln: 3.064 ± 0.027	3.249IleArg: 3.249 ± 0.03	4.793IleSer: 4.793 ± 0.039	3.621IleThr: 3.621 ± 0.028	3.928IleVal: 3.928 ± 0.037	0.734IleTrp: 0.734 ± 0.015	2.282IleTyr: 2.282 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.297LysAla: 4.297 ± 0.042	0.787LysCys: 0.787 ± 0.017	3.832LysAsp: 3.832 ± 0.035	5.414LysGlu: 5.414 ± 0.048	1.995LysPhe: 1.995 ± 0.024	2.886LysGly: 2.886 ± 0.028	1.684LysHis: 1.684 ± 0.02	3.629LysIle: 3.629 ± 0.027	6.103LysLys: 6.103 ± 0.052	5.731LysLeu: 5.731 ± 0.039	1.587LysMet: 1.587 ± 0.019	3.137LysAsn: 3.137 ± 0.026	2.918LysPro: 2.918 ± 0.03	3.331LysGln: 3.331 ± 0.034	4.375LysArg: 4.375 ± 0.04	4.578LysSer: 4.578 ± 0.036	3.76LysThr: 3.76 ± 0.032	3.686LysVal: 3.686 ± 0.033	0.717LysTrp: 0.717 ± 0.013	2.34LysTyr: 2.34 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	6.032LeuAla: 6.032 ± 0.047	1.543LeuCys: 1.543 ± 0.023	5.341LeuAsp: 5.341 ± 0.042	5.934LeuGlu: 5.934 ± 0.044	4.043LeuPhe: 4.043 ± 0.034	4.575LeuGly: 4.575 ± 0.039	2.21LeuHis: 2.21 ± 0.023	5.326LeuIle: 5.326 ± 0.044	6.245LeuLys: 6.245 ± 0.046	9.183LeuLeu: 9.183 ± 0.066	2.175LeuMet: 2.175 ± 0.022	4.375LeuAsn: 4.375 ± 0.038	4.66LeuPro: 4.66 ± 0.037	4.157LeuGln: 4.157 ± 0.037	4.587LeuArg: 4.587 ± 0.038	7.643LeuSer: 7.643 ± 0.048	5.088LeuThr: 5.088 ± 0.039	5.364LeuVal: 5.364 ± 0.04	1.004LeuTrp: 1.004 ± 0.018	3.198LeuTyr: 3.198 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	1.756MetAla: 1.756 ± 0.022	0.305MetCys: 0.305 ± 0.008	1.526MetAsp: 1.526 ± 0.022	1.484MetGlu: 1.484 ± 0.019	0.927MetPhe: 0.927 ± 0.015	1.1MetGly: 1.1 ± 0.019	0.553MetHis: 0.553 ± 0.012	1.542MetIle: 1.542 ± 0.018	1.572MetLys: 1.572 ± 0.022	2.157MetLeu: 2.157 ± 0.022	0.765MetMet: 0.765 ± 0.015	1.39MetAsn: 1.39 ± 0.019	1.11MetPro: 1.11 ± 0.016	0.963MetGln: 0.963 ± 0.017	1.043MetArg: 1.043 ± 0.017	2.069MetSer: 2.069 ± 0.021	1.673MetThr: 1.673 ± 0.022	1.386MetVal: 1.386 ± 0.017	0.221MetTrp: 0.221 ± 0.007	0.768MetTyr: 0.768 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.991AsnAla: 2.991 ± 0.028	0.638AsnCys: 0.638 ± 0.014	2.848AsnAsp: 2.848 ± 0.025	3.143AsnGlu: 3.143 ± 0.032	1.642AsnPhe: 1.642 ± 0.021	2.466AsnGly: 2.466 ± 0.027	1.249AsnHis: 1.249 ± 0.018	3.192AsnIle: 3.192 ± 0.028	3.233AsnLys: 3.233 ± 0.029	3.917AsnLeu: 3.917 ± 0.033	1.285AsnMet: 1.285 ± 0.017	3.504AsnAsn: 3.504 ± 0.053	2.268AsnPro: 2.268 ± 0.027	2.161AsnGln: 2.161 ± 0.024	2.279AsnArg: 2.279 ± 0.025	3.49AsnSer: 3.49 ± 0.029	3.102AsnThr: 3.102 ± 0.028	2.853AsnVal: 2.853 ± 0.028	0.556AsnTrp: 0.556 ± 0.011	1.702AsnTyr: 1.702 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	2.768ProAla: 2.768 ± 0.033	0.539ProCys: 0.539 ± 0.012	2.415ProAsp: 2.415 ± 0.024	3.138ProGlu: 3.138 ± 0.035	1.992ProPhe: 1.992 ± 0.022	2.017ProGly: 2.017 ± 0.025	1.052ProHis: 1.052 ± 0.019	2.922ProIle: 2.922 ± 0.028	2.853ProLys: 2.853 ± 0.031	4.135ProLeu: 4.135 ± 0.034	0.98ProMet: 0.98 ± 0.016	2.235ProAsn: 2.235 ± 0.024	3.088ProPro: 3.088 ± 0.052	1.934ProGln: 1.934 ± 0.029	2.003ProArg: 2.003 ± 0.026	4.568ProSer: 4.568 ± 0.041	3.221ProThr: 3.221 ± 0.035	2.957ProVal: 2.957 ± 0.03	0.476ProTrp: 0.476 ± 0.011	1.601ProTyr: 1.601 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	2.75GlnAla: 2.75 ± 0.031	0.596GlnCys: 0.596 ± 0.011	2.372GlnAsp: 2.372 ± 0.026	3.164GlnGlu: 3.164 ± 0.034	1.696GlnPhe: 1.696 ± 0.019	1.837GlnGly: 1.837 ± 0.022	1.338GlnHis: 1.338 ± 0.021	2.431GlnIle: 2.431 ± 0.025	3.01GlnLys: 3.01 ± 0.029	4.538GlnLeu: 4.538 ± 0.034	1.117GlnMet: 1.117 ± 0.019	1.869GlnAsn: 1.869 ± 0.022	2.255GlnPro: 2.255 ± 0.035	4.426GlnGln: 4.426 ± 0.112	2.443GlnArg: 2.443 ± 0.025	3.171GlnSer: 3.171 ± 0.027	2.417GlnThr: 2.417 ± 0.027	2.582GlnVal: 2.582 ± 0.026	0.525GlnTrp: 0.525 ± 0.012	1.553GlnTyr: 1.553 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	2.972ArgAla: 2.972 ± 0.029	0.706ArgCys: 0.706 ± 0.013	2.491ArgAsp: 2.491 ± 0.025	3.08ArgGlu: 3.08 ± 0.034	2.059ArgPhe: 2.059 ± 0.023	2.236ArgGly: 2.236 ± 0.03	1.486ArgHis: 1.486 ± 0.02	3.181ArgIle: 3.181 ± 0.029	3.784ArgLys: 3.784 ± 0.033	5.031ArgLeu: 5.031 ± 0.037	1.255ArgMet: 1.255 ± 0.017	2.277ArgAsn: 2.277 ± 0.025	2.296ArgPro: 2.296 ± 0.025	2.528ArgGln: 2.528 ± 0.026	3.568ArgArg: 3.568 ± 0.038	3.726ArgSer: 3.726 ± 0.037	2.67ArgThr: 2.67 ± 0.024	2.753ArgVal: 2.753 ± 0.028	0.63ArgTrp: 0.63 ± 0.014	1.833ArgTyr: 1.833 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	4.625SerAla: 4.625 ± 0.041	1.098SerCys: 1.098 ± 0.02	3.941SerAsp: 3.941 ± 0.036	4.053SerGlu: 4.053 ± 0.036	3.426SerPhe: 3.426 ± 0.034	3.624SerGly: 3.624 ± 0.035	1.832SerHis: 1.832 ± 0.024	5.314SerIle: 5.314 ± 0.041	4.863SerLys: 4.863 ± 0.043	7.319SerLeu: 7.319 ± 0.047	1.851SerMet: 1.851 ± 0.02	4.088SerAsn: 4.088 ± 0.037	3.856SerPro: 3.856 ± 0.042	3.16SerGln: 3.16 ± 0.032	3.761SerArg: 3.761 ± 0.038	8.612SerSer: 8.612 ± 0.099	5.437SerThr: 5.437 ± 0.049	4.531SerVal: 4.531 ± 0.037	0.847SerTrp: 0.847 ± 0.015	2.364SerTyr: 2.364 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	4.059ThrAla: 4.059 ± 0.034	0.9ThrCys: 0.9 ± 0.014	2.924ThrAsp: 2.924 ± 0.028	3.509ThrGlu: 3.509 ± 0.033	2.514ThrPhe: 2.514 ± 0.027	3.085ThrGly: 3.085 ± 0.03	1.38ThrHis: 1.38 ± 0.019	4.073ThrIle: 4.073 ± 0.033	3.563ThrLys: 3.563 ± 0.03	5.538ThrLeu: 5.538 ± 0.04	1.345ThrMet: 1.345 ± 0.018	2.776ThrAsn: 2.776 ± 0.025	3.173ThrPro: 3.173 ± 0.038	2.14ThrGln: 2.14 ± 0.022	2.677ThrArg: 2.677 ± 0.025	5.162ThrSer: 5.162 ± 0.048	4.69ThrThr: 4.69 ± 0.073	3.9ThrVal: 3.9 ± 0.037	0.646ThrTrp: 0.646 ± 0.014	1.81ThrTyr: 1.81 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	3.897ValAla: 3.897 ± 0.037	0.967ValCys: 0.967 ± 0.016	3.616ValAsp: 3.616 ± 0.028	3.684ValGlu: 3.684 ± 0.039	2.492ValPhe: 2.492 ± 0.027	2.892ValGly: 2.892 ± 0.031	1.506ValHis: 1.506 ± 0.019	3.899ValIle: 3.899 ± 0.036	3.805ValLys: 3.805 ± 0.03	5.429ValLeu: 5.429 ± 0.04	1.43ValMet: 1.43 ± 0.02	2.833ValAsn: 2.833 ± 0.029	2.866ValPro: 2.866 ± 0.029	2.488ValGln: 2.488 ± 0.026	2.685ValArg: 2.685 ± 0.027	4.334ValSer: 4.334 ± 0.036	3.44ValThr: 3.44 ± 0.03	3.781ValVal: 3.781 ± 0.04	0.69ValTrp: 0.69 ± 0.016	2.221ValTyr: 2.221 ± 0.018	0.0ValXaa: 0.0 ± 0.0
Trp	0.661TrpAla: 0.661 ± 0.014	0.187TrpCys: 0.187 ± 0.007	0.68TrpAsp: 0.68 ± 0.016	0.632TrpGlu: 0.632 ± 0.012	0.529TrpPhe: 0.529 ± 0.012	0.533TrpGly: 0.533 ± 0.013	0.285TrpHis: 0.285 ± 0.009	0.836TrpIle: 0.836 ± 0.015	0.896TrpLys: 0.896 ± 0.015	1.085TrpLeu: 1.085 ± 0.016	0.32TrpMet: 0.32 ± 0.008	0.647TrpAsn: 0.647 ± 0.015	0.418TrpPro: 0.418 ± 0.01	0.454TrpGln: 0.454 ± 0.011	0.62TrpArg: 0.62 ± 0.013	0.94TrpSer: 0.94 ± 0.014	0.717TrpThr: 0.717 ± 0.014	0.61TrpVal: 0.61 ± 0.013	0.188TrpTrp: 0.188 ± 0.007	0.397TrpTyr: 0.397 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.144TyrAla: 2.144 ± 0.024	0.675TyrCys: 0.675 ± 0.017	2.074TyrAsp: 2.074 ± 0.026	2.087TyrGlu: 2.087 ± 0.024	1.627TyrPhe: 1.627 ± 0.02	1.945TyrGly: 1.945 ± 0.025	1.023TyrHis: 1.023 ± 0.016	2.261TyrIle: 2.261 ± 0.025	2.126TyrLys: 2.126 ± 0.022	3.429TyrLeu: 3.429 ± 0.032	0.873TyrMet: 0.873 ± 0.014	1.75TyrAsn: 1.75 ± 0.02	1.613TyrPro: 1.613 ± 0.023	1.607TyrGln: 1.607 ± 0.018	1.825TyrArg: 1.825 ± 0.022	2.511TyrSer: 2.511 ± 0.026	1.965TyrThr: 1.965 ± 0.027	1.979TyrVal: 1.979 ± 0.025	0.453TyrTrp: 0.453 ± 0.011	1.525TyrTyr: 1.525 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 11322 proteins (4154363 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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