Festuca pratensis amalgavirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z6JIN1|A0A2Z6JIN1_9VIRU ORF1p OS=Festuca pratensis amalgavirus 2 OX=2069325 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.35SVDD4 pKa = 3.53PLRR7 pKa = 11.84FLEE10 pKa = 4.96DD11 pKa = 2.99AATAQAADD19 pKa = 4.45AIKK22 pKa = 10.42LRR24 pKa = 11.84PLMLEE29 pKa = 4.08LSGSKK34 pKa = 10.2VLAKK38 pKa = 10.48EE39 pKa = 3.82CDD41 pKa = 3.26IEE43 pKa = 4.27HH44 pKa = 7.07LLNAGFTVDD53 pKa = 5.38DD54 pKa = 4.04VEE56 pKa = 5.02KK57 pKa = 10.58LSKK60 pKa = 10.3SLKK63 pKa = 9.46PLIDD67 pKa = 3.1QGVFDD72 pKa = 5.69DD73 pKa = 4.28AWTMAKK79 pKa = 10.2GSGIILSAQEE89 pKa = 3.87MTFPDD94 pKa = 3.71LFMFRR99 pKa = 11.84RR100 pKa = 11.84WLTTPQGAQALSLVQARR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 5.79MTKK122 pKa = 9.78AGKK125 pKa = 9.67KK126 pKa = 9.5VLGHH130 pKa = 6.0QDD132 pKa = 3.07VALLRR137 pKa = 11.84LLQHH141 pKa = 6.36YY142 pKa = 9.86EE143 pKa = 3.52DD144 pKa = 4.15DD145 pKa = 4.23ARR147 pKa = 11.84RR148 pKa = 11.84EE149 pKa = 3.75LDD151 pKa = 3.14AKK153 pKa = 10.49RR154 pKa = 11.84VEE156 pKa = 4.3TEE158 pKa = 3.62AAVAQLQAEE167 pKa = 4.09IDD169 pKa = 3.8KK170 pKa = 11.04LKK172 pKa = 10.82KK173 pKa = 10.28KK174 pKa = 9.34YY175 pKa = 10.74AKK177 pKa = 10.31AEE179 pKa = 4.13KK180 pKa = 9.31KK181 pKa = 9.8QKK183 pKa = 10.41RR184 pKa = 11.84DD185 pKa = 3.37FPLIANYY192 pKa = 9.96VPLTDD197 pKa = 3.91NEE199 pKa = 4.19VRR201 pKa = 11.84NQAWDD206 pKa = 3.9MYY208 pKa = 9.91CQQCINEE215 pKa = 4.36GSVPMGRR222 pKa = 11.84TSTNLKK228 pKa = 9.96IVGDD232 pKa = 3.93KK233 pKa = 10.78FRR235 pKa = 11.84DD236 pKa = 4.02HH237 pKa = 7.06IVQAHH242 pKa = 6.75KK243 pKa = 9.63LTYY246 pKa = 9.67CQQPDD251 pKa = 3.42HH252 pKa = 6.87TDD254 pKa = 2.92ALIEE258 pKa = 4.24FGKK261 pKa = 10.49QKK263 pKa = 10.34ILRR266 pKa = 11.84CQEE269 pKa = 4.0AGSGKK274 pKa = 10.31LEE276 pKa = 3.95SSFRR280 pKa = 11.84NLLTVLNPEE289 pKa = 4.22NAATLPPRR297 pKa = 11.84TEE299 pKa = 3.92EE300 pKa = 4.38ADD302 pKa = 3.81DD303 pKa = 5.18GVDD306 pKa = 3.44TDD308 pKa = 4.68GGSPNPPEE316 pKa = 5.17PDD318 pKa = 2.95THH320 pKa = 8.17ADD322 pKa = 3.53AEE324 pKa = 4.36PAADD328 pKa = 3.61QPTPPEE334 pKa = 3.8ATGPGSSAGPTPPQSSRR351 pKa = 11.84SHH353 pKa = 5.98KK354 pKa = 9.89RR355 pKa = 11.84KK356 pKa = 9.63QPAVDD361 pKa = 3.79AQSEE365 pKa = 4.38DD366 pKa = 3.76PNPSKK371 pKa = 10.72KK372 pKa = 10.08RR373 pKa = 11.84STRR376 pKa = 11.84AGRR379 pKa = 11.84HH380 pKa = 4.91PNRR383 pKa = 11.84AEE385 pKa = 3.69

Molecular weight:
42.47 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z6JIN1|A0A2Z6JIN1_9VIRU ORF1p OS=Festuca pratensis amalgavirus 2 OX=2069325 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.35SVDD4 pKa = 3.53PLRR7 pKa = 11.84FLEE10 pKa = 4.96DD11 pKa = 2.99AATAQAADD19 pKa = 4.45AIKK22 pKa = 10.42LRR24 pKa = 11.84PLMLEE29 pKa = 4.08LSGSKK34 pKa = 10.2VLAKK38 pKa = 10.48EE39 pKa = 3.82CDD41 pKa = 3.26IEE43 pKa = 4.27HH44 pKa = 7.07LLNAGFTVDD53 pKa = 5.38DD54 pKa = 4.04VEE56 pKa = 5.02KK57 pKa = 10.58LSKK60 pKa = 10.3SLKK63 pKa = 9.46PLIDD67 pKa = 3.1QGVFDD72 pKa = 5.69DD73 pKa = 4.28AWTMAKK79 pKa = 10.2GSGIILSAQEE89 pKa = 3.87MTFPDD94 pKa = 3.71LFMFRR99 pKa = 11.84RR100 pKa = 11.84WLTTPQGAQALSLVQARR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 5.79MTKK122 pKa = 9.78AGKK125 pKa = 9.67KK126 pKa = 9.5VLGHH130 pKa = 6.0QDD132 pKa = 3.07VALLRR137 pKa = 11.84LLQHH141 pKa = 6.36YY142 pKa = 9.86EE143 pKa = 3.52DD144 pKa = 4.15DD145 pKa = 4.23ARR147 pKa = 11.84RR148 pKa = 11.84EE149 pKa = 3.75LDD151 pKa = 3.14AKK153 pKa = 10.49RR154 pKa = 11.84VEE156 pKa = 4.3TEE158 pKa = 3.62AAVAQLQAEE167 pKa = 4.09IDD169 pKa = 3.8KK170 pKa = 11.04LKK172 pKa = 10.82KK173 pKa = 10.28KK174 pKa = 9.34YY175 pKa = 10.74AKK177 pKa = 10.31AEE179 pKa = 4.13KK180 pKa = 9.31KK181 pKa = 9.8QKK183 pKa = 10.41RR184 pKa = 11.84DD185 pKa = 3.37FPLIANYY192 pKa = 9.96VPLTDD197 pKa = 3.91NEE199 pKa = 4.19VRR201 pKa = 11.84NQAWDD206 pKa = 3.9MYY208 pKa = 9.91CQQCINEE215 pKa = 4.36GSVPMGRR222 pKa = 11.84TSTNLKK228 pKa = 9.96IVGDD232 pKa = 3.93KK233 pKa = 10.78FRR235 pKa = 11.84DD236 pKa = 4.02HH237 pKa = 7.06IVQAHH242 pKa = 6.75KK243 pKa = 9.63LTYY246 pKa = 9.67CQQPDD251 pKa = 3.42HH252 pKa = 6.87TDD254 pKa = 2.92ALIEE258 pKa = 4.24FGKK261 pKa = 10.49QKK263 pKa = 10.34ILRR266 pKa = 11.84CQEE269 pKa = 4.0AGSGKK274 pKa = 10.27LEE276 pKa = 4.28SSFVTYY282 pKa = 10.46SLSSIQRR289 pKa = 11.84MLLRR293 pKa = 11.84YY294 pKa = 9.0PLAQRR299 pKa = 11.84KK300 pKa = 7.47QMMEE304 pKa = 5.05WIPMGVVPTRR314 pKa = 11.84PSLTRR319 pKa = 11.84TQMLSQLPTNPLLLKK334 pKa = 10.39PRR336 pKa = 11.84VLGARR341 pKa = 11.84PGPPLPKK348 pKa = 9.97AVEE351 pKa = 4.67AISGNSLLSTPNRR364 pKa = 11.84RR365 pKa = 11.84IQIHH369 pKa = 6.03RR370 pKa = 11.84RR371 pKa = 11.84SGQPGQAGIPTARR384 pKa = 11.84SRR386 pKa = 11.84WEE388 pKa = 3.56AGVRR392 pKa = 11.84HH393 pKa = 6.67IIGGGEE399 pKa = 3.93LLEE402 pKa = 4.09WRR404 pKa = 11.84VDD406 pKa = 3.37NNKK409 pKa = 9.56YY410 pKa = 10.29RR411 pKa = 11.84GGGNLHH417 pKa = 7.22DD418 pKa = 5.07ALLLIGSADD427 pKa = 3.78DD428 pKa = 3.67VTPYY432 pKa = 9.98TALSSLLSVEE442 pKa = 3.9EE443 pKa = 3.8ARR445 pKa = 11.84RR446 pKa = 11.84VLLLPDD452 pKa = 4.17GLAVPDD458 pKa = 4.76GKK460 pKa = 10.69ACCVMKK466 pKa = 10.53QFNDD470 pKa = 3.67DD471 pKa = 3.34ATAGPLLRR479 pKa = 11.84AFGVKK484 pKa = 9.83GKK486 pKa = 10.4YY487 pKa = 8.78GLKK490 pKa = 10.22AAIEE494 pKa = 4.04QFVWGLYY501 pKa = 10.32DD502 pKa = 3.56RR503 pKa = 11.84VGSEE507 pKa = 4.19DD508 pKa = 3.63LKK510 pKa = 11.0PRR512 pKa = 11.84QLPGLLARR520 pKa = 11.84VGYY523 pKa = 7.67RR524 pKa = 11.84TKK526 pKa = 10.96LLDD529 pKa = 3.36MEE531 pKa = 4.67KK532 pKa = 10.61ALKK535 pKa = 9.94KK536 pKa = 9.72IQAVEE541 pKa = 3.78PLGRR545 pKa = 11.84AVMMLDD551 pKa = 3.04ATEE554 pKa = 4.19QCFSSPLFNAISEE567 pKa = 4.55AVTEE571 pKa = 4.06LHH573 pKa = 6.54SNPRR577 pKa = 11.84SGWRR581 pKa = 11.84NYY583 pKa = 9.62LVRR586 pKa = 11.84ASSAWADD593 pKa = 3.14LWDD596 pKa = 3.9EE597 pKa = 4.57LKK599 pKa = 10.87SCGTIVEE606 pKa = 4.5LDD608 pKa = 3.02WSKK611 pKa = 11.06FDD613 pKa = 4.01RR614 pKa = 11.84DD615 pKa = 3.77RR616 pKa = 11.84PAEE619 pKa = 4.6DD620 pKa = 2.75IQFFVDD626 pKa = 4.62VIISCFRR633 pKa = 11.84PKK635 pKa = 10.44NSRR638 pKa = 11.84EE639 pKa = 3.63RR640 pKa = 11.84RR641 pKa = 11.84LLAGYY646 pKa = 10.27RR647 pKa = 11.84KK648 pKa = 9.2MMEE651 pKa = 3.77NALVHH656 pKa = 5.72RR657 pKa = 11.84VMMLDD662 pKa = 3.75DD663 pKa = 4.64GSFFTLEE670 pKa = 3.86GMVPSGSLWTGICDD684 pKa = 3.35TALNIMYY691 pKa = 8.2ITSALRR697 pKa = 11.84KK698 pKa = 9.97LGFDD702 pKa = 4.65DD703 pKa = 4.07STFSPKK709 pKa = 10.26CAGDD713 pKa = 4.26DD714 pKa = 4.03NLTCFQRR721 pKa = 11.84RR722 pKa = 11.84QGLDD726 pKa = 2.88VMEE729 pKa = 4.78RR730 pKa = 11.84LRR732 pKa = 11.84TTLNEE737 pKa = 3.85MFRR740 pKa = 11.84ANIDD744 pKa = 3.3KK745 pKa = 10.77KK746 pKa = 10.94DD747 pKa = 4.53FIVHH751 pKa = 5.56YY752 pKa = 9.38PPYY755 pKa = 10.94AVTTVQACFEE765 pKa = 4.93PGTDD769 pKa = 3.55LSKK772 pKa = 10.44GTSRR776 pKa = 11.84MMDD779 pKa = 3.04KK780 pKa = 11.24AEE782 pKa = 3.99WVPFTGPCPIDD793 pKa = 3.34QAAGRR798 pKa = 11.84SHH800 pKa = 6.25RR801 pKa = 11.84WKK803 pKa = 10.83YY804 pKa = 11.29VFTNKK809 pKa = 9.86PKK811 pKa = 10.24FLANFFLPDD820 pKa = 3.91GKK822 pKa = 9.73PIRR825 pKa = 11.84PAHH828 pKa = 6.75DD829 pKa = 3.76NLEE832 pKa = 4.15KK833 pKa = 10.93LLWPEE838 pKa = 5.16GIHH841 pKa = 6.89EE842 pKa = 4.64SIEE845 pKa = 4.25DD846 pKa = 3.39YY847 pKa = 10.22MAAVLSMVVDD857 pKa = 3.74NPFNHH862 pKa = 6.68HH863 pKa = 5.94NVNHH867 pKa = 5.5MMHH870 pKa = 7.36RR871 pKa = 11.84YY872 pKa = 9.94LIAQQIQKK880 pKa = 10.24QSIFIDD886 pKa = 3.58PAVVMEE892 pKa = 4.45LAKK895 pKa = 10.01TRR897 pKa = 11.84PKK899 pKa = 10.47HH900 pKa = 5.75GEE902 pKa = 3.61AVPFPEE908 pKa = 3.16IAYY911 pKa = 9.24YY912 pKa = 9.86RR913 pKa = 11.84KK914 pKa = 10.02CEE916 pKa = 4.11GYY918 pKa = 10.72VDD920 pKa = 3.9IEE922 pKa = 4.31RR923 pKa = 11.84EE924 pKa = 3.99PEE926 pKa = 3.67FQEE929 pKa = 4.12FFKK932 pKa = 11.02SFRR935 pKa = 11.84EE936 pKa = 4.57FISSVSTLYY945 pKa = 10.78ARR947 pKa = 11.84RR948 pKa = 11.84SEE950 pKa = 4.13GGIDD954 pKa = 2.83AWRR957 pKa = 11.84FMDD960 pKa = 5.13IIRR963 pKa = 11.84GQHH966 pKa = 6.21SIGAGQFGNSVHH978 pKa = 5.32EE979 pKa = 4.07WCRR982 pKa = 11.84FLGEE986 pKa = 4.36HH987 pKa = 6.9PLTRR991 pKa = 11.84SLRR994 pKa = 11.84KK995 pKa = 9.44ARR997 pKa = 11.84RR998 pKa = 11.84FRR1000 pKa = 11.84PMKK1003 pKa = 9.28EE1004 pKa = 3.64AVVADD1009 pKa = 4.24PEE1011 pKa = 4.49TLGKK1015 pKa = 9.95VEE1017 pKa = 5.52KK1018 pKa = 10.85AFTWVLDD1025 pKa = 3.63LCEE1028 pKa = 5.48ANDD1031 pKa = 3.56PMTPLFYY1038 pKa = 10.85ASAVSDD1044 pKa = 3.36ILSSNVSAPP1053 pKa = 3.23

Molecular weight:
118.77 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1438

385

1053

719.0

80.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.11 ± 0.887

1.599 ± 0.148

6.745 ± 0.644

5.981 ± 0.253

3.894 ± 0.639

6.05 ± 0.293

2.225 ± 0.055

4.242 ± 0.554

6.537 ± 0.619

10.083 ± 0.361

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.45

3.06 ± 0.028

6.05 ± 0.602

4.659 ± 0.648

6.815 ± 0.414

5.981 ± 0.131

4.798 ± 0.451

5.633 ± 0.472

1.46 ± 0.336

2.086 ± 0.388

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski