Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Kosmotogales; Kosmotogaceae; Kosmotoga; Kosmotoga olearia

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5CI24|C5CI24_KOSOT Uncharacterized protein OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1) OX=521045 GN=Kole_1101 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.67NFFGPVLLFLVLLFASCHH20 pKa = 6.27PIDD23 pKa = 4.91NPIDD27 pKa = 4.05GDD29 pKa = 4.09GLILFDD35 pKa = 4.61EE36 pKa = 5.06GHH38 pKa = 6.53AQTAGNADD46 pKa = 3.33WVIDD50 pKa = 3.67GGYY53 pKa = 9.94SEE55 pKa = 5.66FADD58 pKa = 3.38ALKK61 pKa = 10.75EE62 pKa = 3.79KK63 pKa = 9.94GYY65 pKa = 10.59SVEE68 pKa = 4.26STDD71 pKa = 5.64DD72 pKa = 3.75PFDD75 pKa = 3.97YY76 pKa = 10.12STLARR81 pKa = 11.84YY82 pKa = 9.41DD83 pKa = 3.41VVIIPEE89 pKa = 3.97PNIRR93 pKa = 11.84FSSDD97 pKa = 3.07EE98 pKa = 3.53ISALKK103 pKa = 10.25RR104 pKa = 11.84YY105 pKa = 9.24VEE107 pKa = 4.23NGGSVFFIGNHH118 pKa = 5.93SGADD122 pKa = 3.65RR123 pKa = 11.84NNDD126 pKa = 2.38GWDD129 pKa = 3.24PVEE132 pKa = 4.39IFNEE136 pKa = 4.23VVNDD140 pKa = 3.71DD141 pKa = 3.43FGFRR145 pKa = 11.84FDD147 pKa = 4.48SNTVSEE153 pKa = 5.34DD154 pKa = 3.84PIEE157 pKa = 5.49DD158 pKa = 3.38ILVTPITYY166 pKa = 9.95GVNAVGMWAGSTITIINDD184 pKa = 3.52SKK186 pKa = 10.05VHH188 pKa = 4.59VAIRR192 pKa = 11.84AYY194 pKa = 8.54EE195 pKa = 3.99KK196 pKa = 10.1PYY198 pKa = 11.02VVYY201 pKa = 9.99GYY203 pKa = 9.92YY204 pKa = 10.91GSGKK208 pKa = 9.57FVAIGDD214 pKa = 4.02SSPFDD219 pKa = 5.35DD220 pKa = 5.62GDD222 pKa = 3.96GDD224 pKa = 4.43PGDD227 pKa = 3.87NLYY230 pKa = 11.21DD231 pKa = 3.14NWYY234 pKa = 10.22DD235 pKa = 3.66YY236 pKa = 11.52DD237 pKa = 4.92DD238 pKa = 4.34SVLAVNIVDD247 pKa = 3.95WLSKK251 pKa = 10.84

Molecular weight: 27.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5CD82|C5CD82_KOSOT Binding-protein-dependent transport systems inner membrane component OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1) OX=521045 GN=Kole_0301 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84VKK11 pKa = 10.48RR12 pKa = 11.84KK13 pKa = 7.72RR14 pKa = 11.84THH16 pKa = 5.76GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 9.87SGRR28 pKa = 11.84RR29 pKa = 11.84IIANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.89GRR39 pKa = 11.84KK40 pKa = 8.69RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2087

0

2087

667979

31

2628

320.1

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.289 ± 0.048

0.749 ± 0.019

5.05 ± 0.043

7.85 ± 0.062

5.065 ± 0.044

6.849 ± 0.051

1.577 ± 0.019

8.258 ± 0.049

7.806 ± 0.051

10.125 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.025

4.13 ± 0.029

3.835 ± 0.03

2.147 ± 0.022

4.894 ± 0.036

6.006 ± 0.04

4.863 ± 0.035

7.285 ± 0.045

1.009 ± 0.02

3.804 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski