Leptospirillum ferrooxidans (strain C2-3)

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Leptospirillum; Leptospirillum sp. Group I; Leptospirillum ferrooxidans

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0IQV0|I0IQV0_LEPFC Putative phage shock protein A OS=Leptospirillum ferrooxidans (strain C2-3) OX=1162668 GN=pspA PE=3 SV=1
MM1 pKa = 7.55HH2 pKa = 7.96LFPMVILSASLLMASCKK19 pKa = 10.43ASNLDD24 pKa = 3.81LSSNGQAGALTSAPTQTATVSGTAVDD50 pKa = 4.12GFIDD54 pKa = 3.36QGEE57 pKa = 4.72VVLSTFPPGAAVLGSATTDD76 pKa = 2.95TSGRR80 pKa = 11.84FSIQTTPIDD89 pKa = 3.66SAALYY94 pKa = 10.4QLTLTGGSSIDD105 pKa = 3.59FASGYY110 pKa = 10.26SISLSSLDD118 pKa = 3.66SLSTIVTGKK127 pKa = 8.12TLLSSPVTITIMTTLMANQAQGMINRR153 pKa = 11.84GAAPLASFQNALGLWSGLLGFDD175 pKa = 3.71PTTTPVTDD183 pKa = 3.77PTAGPEE189 pKa = 4.28SMGYY193 pKa = 8.07PAIYY197 pKa = 10.45GLILAGFSQMAMAIGVNDD215 pKa = 4.1HH216 pKa = 7.0LSPGTSNTLGLLSVLNSDD234 pKa = 3.83ISDD237 pKa = 3.72GVLDD241 pKa = 4.04GLKK244 pKa = 10.59PGDD247 pKa = 4.41PNPLTYY253 pKa = 9.53YY254 pKa = 10.29TYY256 pKa = 10.99TLTADD261 pKa = 3.83TLRR264 pKa = 11.84KK265 pKa = 10.19DD266 pKa = 3.41IADD269 pKa = 3.64STLEE273 pKa = 3.94FLWGDD278 pKa = 3.81RR279 pKa = 11.84NQSGLAPIDD288 pKa = 3.37VSGSVNALALDD299 pKa = 3.92SSSLFPGNPLPTLPDD314 pKa = 4.31PGSPLITVSSPVNNAYY330 pKa = 10.77YY331 pKa = 11.01NNTLPLVATASDD343 pKa = 3.5ALGIGLFSVTSTTLPLPVNPPGSTSIQTGVDD374 pKa = 2.76ILGIPDD380 pKa = 4.02GPLTTVFSAEE390 pKa = 3.94NLAGTVSTQTISLSIDD406 pKa = 3.02NTAPTLQNLSPISATSYY423 pKa = 10.29DD424 pKa = 3.18ICGSTNYY431 pKa = 9.87SVQVTGQMVDD441 pKa = 3.19TGSGPSRR448 pKa = 11.84IQVQEE453 pKa = 3.98TAPVSQAISAYY464 pKa = 7.81STVVNRR470 pKa = 11.84TTSNVSFYY478 pKa = 11.53VFAPVNSPCKK488 pKa = 10.28AVTFTYY494 pKa = 10.79NITVYY499 pKa = 10.71DD500 pKa = 3.78ALNNQRR506 pKa = 11.84TIPYY510 pKa = 10.74SMIIANN516 pKa = 3.85

Molecular weight: 53.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0IN68|I0IN68_LEPFC Putative cation transport protein OS=Leptospirillum ferrooxidans (strain C2-3) OX=1162668 GN=LFE_1014 PE=4 SV=1
MM1 pKa = 7.97WITIGALICFHH12 pKa = 6.84WGSHH16 pKa = 4.68LTSAFAVLGEE26 pKa = 4.47PEE28 pKa = 4.28SSVTADD34 pKa = 3.58RR35 pKa = 11.84EE36 pKa = 4.19RR37 pKa = 11.84SQGSHH42 pKa = 5.51SVRR45 pKa = 11.84TLPGGIRR52 pKa = 11.84MHH54 pKa = 7.28KK55 pKa = 8.08ITTAGGHH62 pKa = 4.65YY63 pKa = 10.08RR64 pKa = 11.84EE65 pKa = 5.37FSGQDD70 pKa = 3.21GAIFAITWRR79 pKa = 11.84GTKK82 pKa = 9.59PSHH85 pKa = 6.94LGDD88 pKa = 3.49LLGAYY93 pKa = 9.38RR94 pKa = 11.84QEE96 pKa = 4.61FIQAVRR102 pKa = 11.84TFRR105 pKa = 11.84MGKK108 pKa = 9.37APNRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84GRR116 pKa = 11.84ILLIQTDD123 pKa = 3.82HH124 pKa = 7.61LLITGTGHH132 pKa = 6.89PRR134 pKa = 11.84DD135 pKa = 3.68LRR137 pKa = 11.84GMVEE141 pKa = 4.85IIGKK145 pKa = 6.68TPPGFDD151 pKa = 3.85PEE153 pKa = 4.91RR154 pKa = 11.84IPPQTPP160 pKa = 2.68

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2413

0

2413

742622

40

1727

307.8

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.122 ± 0.04

0.791 ± 0.017

5.024 ± 0.032

6.412 ± 0.055

4.458 ± 0.037

8.074 ± 0.052

2.295 ± 0.022

6.487 ± 0.04

5.003 ± 0.038

10.877 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.022

3.156 ± 0.031

5.307 ± 0.034

3.105 ± 0.031

6.097 ± 0.044

7.76 ± 0.049

4.951 ± 0.038

6.763 ± 0.049

1.219 ± 0.02

2.429 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski