Rufibacter tibetensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Rufibacter

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0CY26|A0A0P0CY26_9BACT Histidine ammonia-lyase OS=Rufibacter tibetensis OX=512763 GN=DC20_16740 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.54KK3 pKa = 10.47FGLHH7 pKa = 5.24TLLAGLCLFAFSCNEE22 pKa = 4.12EE23 pKa = 4.15EE24 pKa = 4.44PQDD27 pKa = 3.68AVTPSSSKK35 pKa = 10.65EE36 pKa = 3.28ITAAGGIKK44 pKa = 10.29PNGSSLEE51 pKa = 4.09TSASKK56 pKa = 10.05MIQGQDD62 pKa = 3.25LASTSGSCTLIDD74 pKa = 4.22FNSLTSGLLAPGAFAAQGVSITTGPNGTTGSVSVLSGFNLNGCGGPSLQTIPFTGPSVLFNFSEE138 pKa = 4.32PVISVSLTSGDD149 pKa = 3.53FAGDD153 pKa = 3.21EE154 pKa = 4.14DD155 pKa = 5.33VITVRR160 pKa = 11.84AYY162 pKa = 10.48SGEE165 pKa = 4.17NAGGTVLASQTMTLASGLIKK185 pKa = 10.53CLQFYY190 pKa = 11.05LEE192 pKa = 4.13ASGIRR197 pKa = 11.84SVEE200 pKa = 4.02VTSAGIFPNSVFVDD214 pKa = 3.41NLEE217 pKa = 4.13FCLNEE222 pKa = 4.35DD223 pKa = 3.69TDD225 pKa = 4.46SDD227 pKa = 4.31GVNDD231 pKa = 5.79DD232 pKa = 4.47EE233 pKa = 5.87DD234 pKa = 4.06NCPSLSNPGQEE245 pKa = 4.6NNDD248 pKa = 3.22GDD250 pKa = 4.57GLGDD254 pKa = 4.02ACDD257 pKa = 4.58PDD259 pKa = 4.99DD260 pKa = 6.47DD261 pKa = 4.98NDD263 pKa = 4.16GVLDD267 pKa = 4.65ASDD270 pKa = 4.36NCPMLANADD279 pKa = 3.89QLDD282 pKa = 3.95TDD284 pKa = 4.97GDD286 pKa = 4.36GQGNACDD293 pKa = 5.14ADD295 pKa = 4.05DD296 pKa = 5.86DD297 pKa = 4.58NDD299 pKa = 4.17GVVDD303 pKa = 5.19AQDD306 pKa = 4.66CDD308 pKa = 3.87PLSAKK313 pKa = 9.69NDD315 pKa = 3.42KK316 pKa = 10.94VLICHH321 pKa = 7.28KK322 pKa = 10.77GQTLCVAQSAVKK334 pKa = 10.31AHH336 pKa = 6.96LAHH339 pKa = 6.61GDD341 pKa = 3.6EE342 pKa = 5.39VGACGNN348 pKa = 3.73

Molecular weight: 35.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0D403|A0A0P0D403_9BACT Uncharacterized protein OS=Rufibacter tibetensis OX=512763 GN=DC20_21600 PE=4 SV=1
MM1 pKa = 7.44LWLLPVLVLSSCGTARR17 pKa = 11.84RR18 pKa = 11.84SEE20 pKa = 4.77PIMGPLTLQTQQLEE34 pKa = 4.18NGRR37 pKa = 11.84QVFMQNCQRR46 pKa = 11.84CHH48 pKa = 6.31PLGEE52 pKa = 4.88AGLGPSLNNIPLPGAALRR70 pKa = 11.84FRR72 pKa = 11.84VRR74 pKa = 11.84SKK76 pKa = 11.57AFFLGIGRR84 pKa = 11.84MPSFKK89 pKa = 10.02KK90 pKa = 10.13HH91 pKa = 5.49EE92 pKa = 4.13ISRR95 pKa = 11.84EE96 pKa = 3.92QMDD99 pKa = 4.04EE100 pKa = 3.94LVVYY104 pKa = 9.69LKK106 pKa = 10.75ALRR109 pKa = 11.84RR110 pKa = 11.84HH111 pKa = 5.61KK112 pKa = 10.57RR113 pKa = 11.84SEE115 pKa = 3.8AVAAQQ120 pKa = 3.27

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4108

0

4108

1394976

51

5084

339.6

37.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.855 ± 0.035

0.741 ± 0.012

4.886 ± 0.026

6.302 ± 0.047

4.727 ± 0.03

7.156 ± 0.045

2.038 ± 0.018

5.829 ± 0.034

6.07 ± 0.039

9.974 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.019

4.75 ± 0.035

4.336 ± 0.026

4.423 ± 0.033

4.572 ± 0.035

6.15 ± 0.034

5.929 ± 0.054

6.9 ± 0.027

1.258 ± 0.017

3.799 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski